The Hassalľs Bodies

Chapter
Part of the Cancer Growth and Progression book series (CAGP, volume 17)

Abstract

During the thymic ontogenesis, the HBs appear when lymphopoiesis is already established and the cortex, medulla and the cortico-medullary junction are capable of conducting the positive and negative selection of T lymphocytes undergoing progressive maturation. The HBs are structurally organized from RE cells, which usually undergo hypertrophy prior to their inclusion in the outer cell layer of the corpuscles. In our observations the greatest developmental progression and main cell-tissue organization of the HBs was observed between 45 and 54 days of gestation in dogs and between 6 and 10 lunar months in humans. The cellular microenvironment of the thymic medulla is composed of networks of cell types, of a variety of origins, and all of them may participate in the construction of growing, progressive HBs. Histochemically, we detected a rich content of basic non-histone proteins, PAS positive substance (glycogen) and acid mucopolysaccharides within the bodies. Employing the histological stain of Pasini and immunocytochemical methods with monoclonal antibodies (MoABs) AE2 and AE3, high molecular weight (56.5 to 67 kD) basic keratins were defined in human HBs. Employing a panel of MoABs developed against thymic RE cell surface antigens, we observed immunoreactivity localized to the outer cell layer of the HBs with MoABs TE8, TE16 and TE19, while the centrally located cells reacted positively with TE15 and TE19. Immunoreactivity in human skin, employing the TE8, TE16 and TE19 MoABs was also observed in the epidermal granulosa cell layer, while TE15 reacted with cells of the stratum corneum. The presence of endocrine, peptide secreting RE cells within the HBs was defined with the use of MoAB A2B5, which binds to the GQ ganglioside. The hypertrophied, physiologically active RE cells of the peripheral cell layer of the HBs reacted positively with medium to strong intensity when stained with MoABs UJ127.11, J1153, A2B5, 215.D11, and 275.G7. We also observed the expression of transforming growth factor-β type II receptors in HBs. The recently detected expression of the homeobox gene products B3, B4, and C6, transcription factors involved in developmental processes related to hematopoiesis within HBs provides further evidence that HBs are important functional components of the RE network of the thymus which provide developing thymocytes with paracrine and juxtacrine signals to ensure their proper functional maturation during the intrathymic lymphopoiesis. Our transmission electron microscopical (TEM) studies on HBs determined the existence of groups of RE cells connected to one another by desmosomes. We went on to further observe long cytoplasmic processes originating from medullary RE cells and directly contacting thymic T lymphocytes and accessory antigen presenting cells (macrophages, dendritic cells, interdigitating cells, Langerhans cells, etc.) by the use of scanning electron microscopy (SEM). Thus, our results indicate that the HBs are unique, antigenically distinct, functionally active, multicellular components of the nonlymphocytic, cellular microenvironment of the thymic medulla, and participate in the physiological activities of the prenatal and adult thymus. Future immunohistochemical and genetic studies will clarify the exact origin of the various non-lymphatic thymic cells participating in the determination of the particular physiological activities, progressive growth, and the terminal cell differentiation within the HBs.

Key words

Thymic Ontogenesis in Vertebrates Origin of the Hassalľs Bodies (HBs)—Hypotheses Reticulo-Epithelial Cell Origin of HBs Capillary Involvement Morphological Observations Scanning Electron microscope (SEM) Study Transmission Electron microscope (TEM) Observation Immunocytochemistry Streptavidin-biotin antigen detection technique Mouse Anti-Human Monoclonal Antibody (MoAB) Immunophenotype (IP) Thymic Organ and Tissue Cultures Total Body and Local Thymic Irradiation Thymic involution 
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References

  1. 1.
    Miller JFAP: Immunological function of the thymus. Lancet II: 748–749, 1961.Google Scholar
  2. 2.
    Hoshino T, Takeda M, Abe K, Ito T: Early development of thymic lymphocytes in mice studied by light and electron microscopy. Anat Rec 164: 47–65, 1969.PubMedCrossRefGoogle Scholar
  3. 3.
    Stutman O: Intrathymic and extrathymic T cell maturation. Immunol Rev 42: 138–184, 1978.PubMedGoogle Scholar
  4. 4.
    Bodey B: Development of lymphopoiesis as a function of the thymic microenvironment. Use of CD8+ cytotoxic T lymphocytes for cellular immunotherapy of human cancer. IN VIVO 8: 915–943, 1994.PubMedGoogle Scholar
  5. 5.
    Jolly J: Sur les organes lympho-epitheliaux. Compt Rend Soc Biol 74: 540–543, 1913.Google Scholar
  6. 6.
    Grégoire C: Recherches sur le symbiose lymphoepitheliale au niveau du thymus de mammifere. Arch Biol 46: 717–820, 1935.Google Scholar
  7. 7.
    Grégoire C, Duchateau G: A study on lympho-epithelial symbiosis in thymus. Reactions of the lymphatic tissue to extracts and to implants of epithelial components of thymus. Arch Biol 68: 269–296, 1956.Google Scholar
  8. 8.
    Saad AH, Zapata A: Reptilian thymus gland: an ultrastructural overview. Thymus 20: 135–152, 1992.PubMedGoogle Scholar
  9. 9.
    Hassall AH: The microscopic anatomy of the human body, in health and disease. Vol.1. London, 32 Fleet Street, Samuel Highley, 1849, pp 3–12 & 477–479.Google Scholar
  10. 10.
    Henle J: Ueber Hassalľs konzentrische Körperchen des Blutes. Z Radiation Med 7, 1849.Google Scholar
  11. 11.
    Gray E: By candlelight. The life of Dr. Arthur Hill Hassall. London, Robert Hale, 1983.Google Scholar
  12. 12.
    Ortiz-Hidalgo C: Early clinical pathologists 5: The man behind Hassalľs corpuscles. J Clin Pathol 45: 99–101, 1992.PubMedGoogle Scholar
  13. 13.
    Bruch: Zeitschrift Radiation. Med 9, 1850.Google Scholar
  14. 14.
    Friedleben A: Die Physiologie der Thymusdrüse in Gesundheit und Krankheit. Frankfurt a/M, 1858, pp 1–336.Google Scholar
  15. 15.
    Maurer F: Die Schilddrüse, Thymus und andere Schlundspaltenderivate bei der Eidesche. Morph Jahrbuch 27: 119–172, 1899.Google Scholar
  16. 16.
    Magni S: über einige histologische Untersuchungen der normalen Thymusdrüsen eines fünfmonatlichen und eines reifen Fetus. Arch Kinderheilk 38: 14–17, 1903.Google Scholar
  17. 17.
    Stöhr Ph: über die Natur der Thymuselemente. Anat Hefte 31: 409–457, 1906.Google Scholar
  18. 18.
    Stöhr Ph: über die Abstammung der kleinen Thymusrindenzellen. Anat Hefte 41: 109–127, 1910.Google Scholar
  19. 19.
    Fulci F: Die Natur der Thymusdrüse nach Untersuchungen über ihre Regenerationsfähigkeit bei Säugetieren. Deutsch Med Wochenschr 39: 1776–1780, 1913.CrossRefGoogle Scholar
  20. 20.
    Ghika C: étude sur le thymus. Paris, Thèse, 1901.Google Scholar
  21. 21.
    Goldner J: Action de ľadrénaline sur le thymus. C R Soc Biol (Paris) 88: 545–548, 1923.Google Scholar
  22. 22.
    Goldner J: Histogénèse du corpuscule de Hassall: unité cytogénique des cellules de charpente, des placards épithéliaux, des corpuscules unicellulaires et des corpuscules hassalliens. C R Soc Biol (Paris) 88: 947–950, 1923.Google Scholar
  23. 23.
    Cornil V, Ranvier L: Manuel ďhistologie Pathologique. Paris, 1869, pp 133–136.Google Scholar
  24. 24.
    Afanassiew B., Ueber die konzentrischen Körper des Thymus. Arch Mikr Anat 14: 320–342, 1877.Google Scholar
  25. 25.
    Nusbaum J, Machowski J: Die Bildung der concentrischen Körperchen und die phagocytotischen Vorgänge bei der Involution des Amphibienthymus nebst einigen Bemerkungen über die Kiemenreste und Epithelkörperchen der Amphibien. Anat Anz 21: 110–127, 1902.Google Scholar
  26. 26.
    Jordan HE, Horsley GW: The significance of the concentric corpuscles of Hassall. Anat Rec 35: 279–307, 1927.Google Scholar
  27. 27.
    Juba A, Mihàlik P von: über die Entstchung der Hassallschen Körperchen. Z Anat 90: 278–287, 1927.Google Scholar
  28. 28.
    Jordan HE, Looper JB: The histology of the thymus gland of the box-turtle, Terrapene carolina, with special reference to the concentric corpuscles of Hassall and the eosinophilic granulocytes. Anat Rec 40: 309–337, 1928.CrossRefGoogle Scholar
  29. 29.
    Kölliker A von: Entwicklungsgeschichte des Menschen und der höheren Tiere. Leipzig, W Engleman Publ, 1861.Google Scholar
  30. 30.
    Kölliker A von: Entwicklungsgeschichte des Menschen und der höheren Tiere. 2nd Aufl, Leipzig, W Engleman Publ, 1879.Google Scholar
  31. 31.
    His W: Zur Anatomie der menschlichen Thymusdrüse. Z Zool 11: 164, 1862.Google Scholar
  32. 32.
    His W: Anatomie menschlicher Embryonen. Leipzig, 1880.Google Scholar
  33. 33.
    Toldt: Ueber lymphoide Organe der Amphibien. Sitzungsber Akad Wissensch 58 (2), 1868.Google Scholar
  34. 34.
    Krause C: Handbuch des Menschen. Hannover, 1876, p 359.Google Scholar
  35. 35.
    Stieda L: Untersuchungen ueber die Entwicklung der Glandula thymus, Glandula thyreoidea und Glandula carotica. Leipzig, 4: 1–38, 1881.Google Scholar
  36. 36.
    Capobianco F: Della natura dei corpuscoli di Hassall, contribuzione alle conoscenze morphologiche del timo. Boll Soc Natur Napoli 1(4), 1890.Google Scholar
  37. 37.
    Pierson G: Ueber die Entwicklung der embryonalen Schlundspalten und ihre Derivate bei Säugethieren. Zeitschr Zool 47, 1899.Google Scholar
  38. 38.
    Ebner V von: Der Thymus. In: Handbuch der Gewebelehre des Menschen. (Koelliker A, ed) Leipzig, 1899, p 328.Google Scholar
  39. 39.
    Prymak T: Beiträge zur Kenntnis des feineren Baues und der Involution der Thymusdrüse bei den Teleostieren. Anat Anz 21, 1902.Google Scholar
  40. 40.
    Wallish M: Zur Bedeutung der Hassallschen Körperchen. Arch Mikr Anat 63: 274–282, 1903.Google Scholar
  41. 41.
    Goodall A: The postnatal changes in the thymus of guineapigs and the effect of castration on thymus structure. J Physiol 32: 191–198, 1905.PubMedGoogle Scholar
  42. 42.
    Klose H, Vogt H: Klinik und Biologie der Thymusdrüse. Beitr Klin. Chir 69: 1–200, 1910.Google Scholar
  43. 43.
    Rabl H: Die Entwicklung der Derivate des Kiemendarmes beim Meerschweinchen. Arch Mikr Anat 82: 79–147.Google Scholar
  44. 44.
    Hart C: Thymusstudien IV. Die Hassallschen Körperchen. Virch Arch Pathol Anat 217: 239–266, 1914.Google Scholar
  45. 45.
    Marine D: The frequency of duct-like spaces in the thymus gland, with remarks on the formation and fate of Hassalľs corpuscles. Cleveland Med J 14: 186–195, 1915.Google Scholar
  46. 46.
    Scammon RE: The prenatal growth of the human thymus. Proc Soc Exp Biol Med 24: 906–909, 1927.Google Scholar
  47. 47.
    Woollard HH: The potency of the pharyngeal endoderm. J Anat 66: 242–260, 1932.PubMedGoogle Scholar
  48. 48.
    Webster WD: The development of the thymus bodies in Necturus maculosus. J Morphol 56: 295–323, 1934.CrossRefGoogle Scholar
  49. 49.
    Schambacher A: über die Pericyten von Drüsenkanälen der im Thymus und ihre Beziehung zur Entstehung der Hassalschen Körperchen. Virch Arch 172: 368–394, 1903.Google Scholar
  50. 50.
    Shier KJ: The morphology of the epithelial thymus. Observations on lymphocyte-depleted and fetal thymus. Lab Invest 12: 316–326, 1963.PubMedGoogle Scholar
  51. 51.
    Hammar JA: Die normal-morphologische Thymusforschung im letzten Vierteljahrhundert. Analyse und Synthese. Leipzig, Barth, 1936.Google Scholar
  52. 52.
    Kostowiecki M: über die Beziehung der Hassallschen Körperchen zu den benachbarten Blutgefässen in dem Thymus menschlicher Phoeten. Bull Int Acad Polon Sci Cracovie Sc Nat Series B: 589–628, 1930.Google Scholar
  53. 53.
    Weller GL: Development of the thyroid, parathyroid and thymus glands in man. Contrib Embryol 24: 95–138, 1933.Google Scholar
  54. 54.
    Kostowiecki M: Development and degeneration of the second type of Hassalľs corpuscles in the thymus of guinea pig. Anat Rec 142: 195–203, 1962.PubMedCrossRefGoogle Scholar
  55. 55.
    Norris EH: The morphogenesis and histogenesis of the thymus gland in man: in which the origin of the Hassalľs corpuscles of the human thymus is discovered. Contrib Embryol 27: 191–208, 1938.Google Scholar
  56. 56.
    Zottermann A: Die Schweinethymus als eine Thymus Ectoentodermalis. Anat Anz 38: 20–21 and 514–530, 1911.Google Scholar
  57. 57.
    Massart C: Morfologia e sviluppo del sistema tireo-paratireoideo con ricerche originali nei chirotteri (Vesperugo pipistrellus). Arch Ital Anat 44: 22–79, 1940.Google Scholar
  58. 58.
    Massart C: Morfologia e sviluppo del timo con ricerche originali nei chirotteri (Vesperugo pipistrellus). Arch Ital Anat 44: 489–550, 1940.Google Scholar
  59. 59.
    Winiwarter H de: Origine des corps de Hassall du thymus des mammifères (Chien et Chat). C R Soc Biol (Paris) 89: 834–836, 1923.Google Scholar
  60. 60.
    Dustin AP, Baillez G: ľorigine des corps de Hassall et des formations kystiques du thymus des mammifères. Bull Soc R SC Med Nat 2: 44–46, 1914.Google Scholar
  61. 61.
    Imai M, Shibata T, Mineda T: A new opinion on the origin of Hassalľs corpuscles. Okajimas Fol Anat Jap 45: 51–69, 1968.Google Scholar
  62. 62.
    Wassjutotschkin AM: Untersuchungen über die Histogenese des Thymus. 3. Ueber die myoiden Elemente des Thymus des Menschen. Anat Anz 50: 547–551, 1918.Google Scholar
  63. 63.
    Kliwanskaja-Kroll E: Zur Morphologie des experimentellen Hyperthyreoidismus. Das inkretorische System des im Wachstum begriffenem Organismus bei systematischer Fütterung mit Schilddrüsensubstanz (Schilddrüse und Thymusdrüse). Virch Arch Pathol Anat 272: 430–441, 1929.CrossRefGoogle Scholar
  64. 64.
    Bastenie P: La genèse des corps de Hassall dans les thymus humains pathologiques. C R Soc Biol (Paris) 106: 55–56, 1931.Google Scholar
  65. 65.
    Bastenie P: Contribution a ľanatomie pathologique des thymus humains. Arch Int Med Exp 7: 273–345, 1932.Google Scholar
  66. 66.
    Paulitzky A: Disquisitiones de stratis glandulae thymi corpusculis. Halis Habilitationsschr 1863.Google Scholar
  67. 67.
    Watney H: Note on the minute anatomy of the thymus. Proc Roy Soc 27, 1878.Google Scholar
  68. 68.
    Watney H: The minute anatomy of the thymus. Phil Trans Roy Soc 3: 1063–1123, 1882.Google Scholar
  69. 69.
    Tourneux F, Verdun P: Sur les premiers developments de la thyroide, du thymus et des glandules parathyroidennes chez ľhomme. J de ľAnat 33: 305–325, 1897.Google Scholar
  70. 70.
    Grégoire C: Action des rayons X sur le thymus au cours de ľhistogénèse. C R Assoc Anat (Paris) 27: 328–335, 1932.Google Scholar
  71. 71.
    Béclère H, Pigache R: Action des rayons de Roentgen sur les corpuscles de Hassall. Bull Soc Anat (Paris) 86: 47–51, 1911.Google Scholar
  72. 72.
    Pigache R, Worms G: Le thymus considéré comme glande à secrétion interne. C R Acad Sci (Paris) 154: 234–236, 1912.Google Scholar
  73. 73.
    Kingsbury BF: The development of the human pharynx. I. The pharyngeal derivatives. Am J Anat 18: 329–398, 1915.CrossRefGoogle Scholar
  74. 74.
    Kingsbury BF: On the nature and significance of the thymic corpuscles (of Hassall). Anat Rec 39: 141–159, 1928.Google Scholar
  75. 75.
    Kingsbury BF: On the mammalian thymus, particularly thymus IV: the development in the calf. Am J Anat 60: 149–183, 1936.CrossRefGoogle Scholar
  76. 76.
    Beard J: The origin and histogenesis of the thymus in Raja batis. Zool Jahrbücher 17: 403–480, 1902.Google Scholar
  77. 77.
    Popoff N: The histogenesis of thymus as shown by tissue culture. Arch J Exptl Zellforsch 4: 395–416, 1926.Google Scholar
  78. 78.
    Popoff NW: The histogenesis of the thymus as shown by tissue cultures, transplantation and regeneration. Proc Soc Exper Biol Med 24: 148–151, 1926.Google Scholar
  79. 79.
    Hammar JA: Zur Histogenese und Involution der Thymusdrüse. Anat Anz 27: 23–30 & 41–89, 1905.Google Scholar
  80. 80.
    Hammar JA: Fünfzig Jahre Thymusforschung. Ergebn Anat Entwicklunggesch 19: 1–274, 1909.Google Scholar
  81. 81.
    Hammar JA: Methode, die Menge der Rinde und des Marks der Thymus, sowie die Anzahl und die Größe der Hassallschen Körper zahlenmäßig festzustellen. Z Angew Anat 1: 311–396, 1914.Google Scholar
  82. 82.
    Hammar JA: Die Menschenthymus in Gesundheit und Krankheit: Ergebnisse der numerischen Analyse von mehr als tausend menschlichen Thymusdrüsen. Teil I. Das normale Organ—zugleich eine kritische Beleuchtung der Lehre des “Status thymicus”. Z Mikr Anat Forsch (Leipzig) 6: 1–570, 1926.Google Scholar
  83. 83.
    Prenant A: Contribution a ľétude du développement organique et histologique du thymus, de la glande thyroide et de la glande carotidienne. La Cellule 10: 85–184, 1894.Google Scholar
  84. 84.
    Bell ET: The development of the thymus. Am J Anat 5: 29–62, 1905.CrossRefGoogle Scholar
  85. 85.
    Dantschakoff V: Untersuchungen von Blut und Bindegewebe bei Vögeln. Arch Mikr Anat 73: 117–181, 1908.Google Scholar
  86. 86.
    Mietens H: Zur Kenntnis des Thymusretikulum und seiner Beziehungen zu dem der Lymphdrüsen nebst einigen Bemerkungen über die Winterschlafdrüse. Jenaische Zeitschrift Naturwissensch 44: 149, 1908.Google Scholar
  87. 87.
    Maximow AA: Untersuchungen uber Blut und Bindegewebe. II. Uber die Histogenese der Thymus bei Saugetieren. Arch mikr Anat 74: 525–621, 1909.Google Scholar
  88. 88.
    Pappenheimer AM: A contribution to the normal and pathological histology of the thymus gland. J Med Res 22: 1–73, 1910.PubMedGoogle Scholar
  89. 89.
    Pappenheimer AM: Further studies of the histology of the thymus. Am J Anat 14: 299–325, 1913.CrossRefGoogle Scholar
  90. 90.
    Jaffe HL, Plavska A: Experimental studies on the formation of Hassalľs corpuscles. Proc Soc Exper Biol Med 23: 91–93, 1925.Google Scholar
  91. 91.
    Smith C: The thymus in immunobiology. In: The Thymus in Immunobiology. Structure, function and role in disease. (Good RA, Gabrielsen AE, eds) New York, Harper & Row, Inc., 1964.Google Scholar
  92. 92.
    Bodey B: Histomorphology and histochemistry of the human thymus during its prenatal ontogenesis. Dissertation, Inst Morphol, Bulg Acad Sci, Sofia, Bulgaria, 1977, pp 1–360.Google Scholar
  93. 93.
    Bodey B, Hadjioloff AI: Thymus development, structure and function. Nature (Bulgaria) 26: 11–19, 1977.Google Scholar
  94. 94.
    Liberti EA, Fagundes TP, Perito MA, Matson E, König Junior B: On the size of Hassalľs corpuscles in human fetuses. Bull Assoc Anat 78: 15–18, 1994.Google Scholar
  95. 95.
    Kingsbury BF: The interpretation of thymus bodies. Endocrinology 29: 155–160, 1941.CrossRefGoogle Scholar
  96. 96.
    Hartmann A: Die Entwicklung der Thymus beim Kaninchen. Arch Mikr Anat 86: 69–192, 1914.Google Scholar
  97. 97.
    Bodey B, Calvo W, Prummer O, Fliedner TM, Borysenko M: Development and histogenesis of the thymus in dog. A light and electronmicroscopical study. Dev Comp Immunol 11: 227–238, 1987.PubMedGoogle Scholar
  98. 98.
    Hammar JA: über progressive und regressive Formen von Hassallschen Körpern. Z Anat 70: 466–488, 1924.CrossRefGoogle Scholar
  99. 99.
    Lobach DF, Scearce RM, Haynes BF: The human thymic microenvironment. Phenotypic characterization of Hassalľs bodies with the use of monoclonal antibodies. J Immunol 134: 250–257, 1985.PubMedGoogle Scholar
  100. 100.
    Hammar JA: Zur Frage der Histogenese der Thymusdrüse. Zbl Pathol 33: 505–513, 1923.Google Scholar
  101. 101.
    Caso LV: Histochemical studies of mucosubstances and keratins of thymic corpuscles. Aust J Exp Biol Med Sci 57: 493–496, 1979.PubMedGoogle Scholar
  102. 102.
    Smith C, Parkhurst HT: Studies on the thymus of the mammal. XI. A comparison of the staining properties of Hassalľs corpuscles and of thick skin of the guinea pig. Anat Rec 103: 649–673, 1949.CrossRefPubMedGoogle Scholar
  103. 103.
    Gaudecker B von, Schmale EM: Similarities between Hassalľs corpuscles of the human thymus and the epidermis. An investigation by electron microscopy and histochemistry. Cell Tissue Res 151: 347–368, 1974.CrossRefGoogle Scholar
  104. 104.
    Baker JR: Cytological technique. 2nd Ed. London, Methuen, 1946, pp 1–120.Google Scholar
  105. 105.
    Baker JR: Principles of biological microtechnique. A study of fixation and dyeing. London, Methuen, 1958.Google Scholar
  106. 106.
    Jones W: On the enzyme of the thymus. Am J Physiol 10: 24–25, 1903.Google Scholar
  107. 107.
    Kouvalainen K: Some organ antibodies in congenital nephrosis. A preliminary report. Ann Paediat Fenn 7: 165–168, 1961.PubMedGoogle Scholar
  108. 108.
    Pearson B, Wolf P, Andrews M: The histochemical demonstration of leucine aminopeptidase by means of a new indolyl compound. Lab Invest 12: 712–720, 1963.PubMedGoogle Scholar
  109. 109.
    Kouvalainen K, Wallgren GR: The thymus in myasthenia gravis with special reference to the significance of Hassalľs corpuscles. Ann Med Exp Biol Fenn 45: 112–116, 1967.PubMedGoogle Scholar
  110. 110.
    Timperley WR, Ahmed A: Histochemistry of the thymus and a thymoma. Arch Pathol 89: 405–413, 1970.PubMedGoogle Scholar
  111. 111.
    Timperley WR, Barson AJ, Davies S: Enzyme histochemistry of the developing human thymus. Biol Neonate 19: 42–54, 1971.PubMedGoogle Scholar
  112. 112.
    Vetters JM, Macadam RF: Fine structural evidence for hormone secretion by the human thymus. J Clin Pathol 26: 194–197, 1973.PubMedGoogle Scholar
  113. 113.
    Fennel RH Jr, Reddy CR, Vazquez JJ: Progressive systematic sclerosis and malignant hypertension. Immunohistochemical study of renal lesions. Arch Pathol 72: 209–215, 1961.Google Scholar
  114. 114.
    Arvy L: Histoenzymology of the parathyroid gland in Ovis Aries L. C R Soc Biol 155: 2094–2098, 1961.Google Scholar
  115. 115.
    Kouvalainen K: Species and organ dependence of alkaline phosphatase activity in lymphatic tissues. A histochemical, biochemical and electrophoretic study. Histochem J 3: 55–96, 1971.PubMedCrossRefGoogle Scholar
  116. 116.
    Arvy L: Enzymology of the thymus. In: Handbuch der Histochemie. Vol. 8. Stuttgart, Gustav Fischer, 1973.Google Scholar
  117. 117.
    Navaro D: The thymus gland of birds and the arylsulfatase activity (ASA) in its parenchyma. Arch Ita Anat Embriol 84: 265–283, 1979.Google Scholar
  118. 118.
    Ruuskanen O, Toivanen A, Rackallio J: Histochemical characterization of chicken lymphoid tissue. Dev Comp Immunol 1: 231–240, 1977.PubMedGoogle Scholar
  119. 119.
    D’Anna F: Histochemical studies of the peroxidase activity of the bursa of Fabricius of Gallus domesticus; study of the enzymatic metabolism of that immunocompetent organ during its development. Rivista Istochim, Norm Patol 20: 48–60, 1976.Google Scholar
  120. 120.
    D’Anna F, Rossi F, Arbico R: Hassalľs corpuscle: a regressive formation. Basic Appl Histochem 25: 169–181, 1981.Google Scholar
  121. 121.
    Gori P: Ferritin in the integumentary cells of Oxalis corniculata. J Ultrastruct Res 60: 95–98, 1977.PubMedCrossRefGoogle Scholar
  122. 122.
    Gomori G: The distribution of phosphatase in normal organs and tissues. J Cell Comp Physiol 17: 71–83, 1941.CrossRefGoogle Scholar
  123. 123.
    Gomori G: Histochemistry of enzymes. J Mt Sinai Hosp 19: 446–451, 1952.Google Scholar
  124. 124.
    Posalaky Z, Gyevai A, Bukulya B: Data on the mechanism of spermatogenesis based on studies with acid-fast dyes on lipid and succinic dehydrogenase. Kiserl Orvostud 13: 35–48, 1961.PubMedGoogle Scholar
  125. 125.
    Barka T, Anderson PJ: Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler. J Histochem Cytochem 10: 741–753, 1962.Google Scholar
  126. 126.
    Smith C: Studies on the thymus of the mammal. XV. Sites of aminopeptidase activity in the thymus of the mouse. Anat Rec 158: 149–161, 1967.PubMedGoogle Scholar
  127. 127.
    Korhonen LK, Ruponen B: Leucine aminopeptidase in the thymus. Acta Anat 50: 186–190, 1962.PubMedCrossRefGoogle Scholar
  128. 128.
    Hess EL, Lagg SE: The chemical fractionation of rabbit and swine thymuses. J Biophys Biochem Cyto 3: 812–815, 1958.Google Scholar
  129. 129.
    Pearse AGE: Histochemistry: Theoretical and applied. London, Churchill, 1960.Google Scholar
  130. 130.
    Hirokawa K, Saitoh K, Hatakeyama S: Enzyme histochemical study on human thymus and its age change. Acta Pathol Jap 33: 275–285, 1983.Google Scholar
  131. 131.
    D’Anna F, Morano GP: Non-specific esterase activity in birds’ lymphoid organs. Comparative histochemical research. Cell Molec Biol 28: 457–464, 1982.Google Scholar
  132. 132.
    Brody IA: Histochemistry of lactate dehydrogenase isozymes in intact muscle. Ann NY Acad Sci 151: 587–593, 1968.PubMedGoogle Scholar
  133. 133.
    Barnett RJ, Seligman AM: Histochemical demonstration of sulfhydryl and disulfide groups of proteins. J Natl Cancer Inst 14: 769–803, 1954.Google Scholar
  134. 134.
    Soderstrom KO, Rinne R, Hopsu-Havu VK, Jarvinen M, Rinne A: Identification of acid cysteine proteinase inhibitor (cystatin A) in the human thymus. Anat Rec 240: 115–119, 1994.PubMedGoogle Scholar
  135. 135.
    Bodey B, Calvo W: Histochemistry of the Hassalľs bodies in the thymuses of dog and human fetuses during prenatal development. Abstract, 7th Internat Congress Histochem Cytochem, Helsinki, 1984.Google Scholar
  136. 136.
    Burfeind P, Hoyer-Fender S, Doenecke D, Tsaousidou S, Engel W: Expression of a histone H1 gene 9H1.1 in human testis and Hassalľs corpuscles of the thymus. Thymus 19: 245–251, 1992.PubMedGoogle Scholar
  137. 137.
    Pertschuk LP: Immunofluorescence of Hassalľs corpuscles with pemphigus serum. Confirmation of their squamous epithelial nature. Tissue Antigens 4: 446–451, 1974.Google Scholar
  138. 138.
    Pertschuk LP, Rosen Y: An immunofluorescent study of tumors with specific antisera for squamous epithelial intercellular substance and basement membrane. Am J Clin Pathol 60: 601–607, 1973.PubMedGoogle Scholar
  139. 139.
    Beutner EH, Lever WF, Witebsky E, Jordon RE, Chertock B: Autoantibodies in pemphigus vulgaris. Responses to an intercellular substance of epidermis. J Am Med Assoc 192: 682–688, 1965.Google Scholar
  140. 140.
    Monier JC, Dardenne M, Pléau JM, Schmitt D, Deschaux P, Bach JF: Characterization of facteur thymique sérique (FTS) in the thymus. I. Fixation of anti-FTS antibodies on thymic reticuloepithelial cells. Clin Exp Immunol 42: 470–476, 1980.PubMedGoogle Scholar
  141. 141.
    Schmitt D, Monier JC, Dardenne M, Pleau JM, Deschaux P, Bach JF: Cytoplasmic localization of FTS (facteur thymique sérique) in thymic epithelial cells. An immunoelectron microscopical study. Thymus 2: 177–186, 1980.PubMedGoogle Scholar
  142. 142.
    Schmitt D, Monier JC, Dardenne M, Pleau JM, Bach JF: Location of FTS (facteur thymique serique) in the thymus of normal and auto-immune mice. Thymus 4: 221–231, 1982.PubMedGoogle Scholar
  143. 143.
    Dalakas MC, Engel WK, McClure JE, Goldstein A, Askanas V: Immunocytochemical characterization of thymosin in thymic epithelial cells of normal and myasthenia gravis patients and in thymic cultures. J Neurol Sci 50: 239–247, 1981.PubMedCrossRefGoogle Scholar
  144. 144.
    Savino W, Dardenne M: Thymic hormone containing cells. VI. Immunohistologic evidence for the simultaneous presence of thymulin, thymopoietin and thymosin α1 in normal and pathological human thymuses. Eur J Immunol 14: 987–992, 1984.PubMedGoogle Scholar
  145. 145.
    Drenckhahn D, von Gaudecker B, Müller-Hermelink HK, Unsicker K, Groschel-Stewart U: Myosin and actin containing cells in the human postnatal thymus. Ultrastructural and immunhistochemical findings in normal thymus and in myasthenia gravis. Virchows Arch B Cell Pathol 32: 33–45, 1979.Google Scholar
  146. 146.
    Takigawa M, Imamura S, Ofuji Sh: Demonstration of epidermis-specific heteroantigens in thymic epithelial cells. Int Arch Allergy Appl Immunology 55: 58–60, 1977.Google Scholar
  147. 147.
    Laster AJ, Itoh T, Palker TJ, Haynes BF: The human thymic microenvironment. Thymic epithelium contains specific keratins associated with early and late stages of epidermal keratinocyte maturation. Differentiation 31: 67–77, 1986.PubMedGoogle Scholar
  148. 148.
    Janossy G, Thomas JA, Bollum FJ, Granger S, Pizzolo G, Bradstock KF, Wong L, McMichael A, Ganeshaguru K, Hoffbrand AV: The human thymic microenvironment: an immunohistologic study. J Immunol 125: 202–212, 1980.PubMedGoogle Scholar
  149. 149.
    McFarland EJ, Scearce RM, Haynes BF: The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J Immunol 133: 1241–1249, 1984.PubMedGoogle Scholar
  150. 150.
    Haynes BF: The human thymic microenvironment. Adv Immunol 36: 87–142, 1984.PubMedGoogle Scholar
  151. 151.
    van Vliet E, Melis M, Ewijk W: Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 14: 524–529, 1984.PubMedGoogle Scholar
  152. 152.
    Kendall MD, van de Wijngaert FP, Schuurman HJ, Rademakers LH, Kater L: Heterogeneity of the human thymus epithelial microenvironment at the ultrastructural level. Adv Exp Med Biol 186: 289–297, 1985.PubMedGoogle Scholar
  153. 153.
    de Maagd RA, MacKenzie WA, Schuurman HJ, Ritter MA, Price KM, Broekhuizen R, Kater L: The human thymus microenvironment: heterogeneity detected by monoclonal anti-epithelial cell antibodies. Immunology 54: 745–754, 1985.PubMedGoogle Scholar
  154. 154.
    Ritter MA, Schuurman HJ, MacKenzie WA, de Maagd RA, Price KM, Broekhuizen R, Kater L: Heterogeneity of human thymus epithelial cells revealed by monoclonal anti-epithelial cell antibodies. Adv Exp Med Biol 186: 283–288, 1985.PubMedGoogle Scholar
  155. 155.
    von Gaudecker B, Steinmann GG, Hansmann ML, Harpprecht J, Milicevic NM, Müller-Hermelink HK: Immunocytochemical characterization of the thymic microenvironment. A light-microscopic and ultrastructural immunocytochemical study. Cell Tissue Res 244: 403–412, 1986.CrossRefGoogle Scholar
  156. 156.
    Lobach DF, Haynes BF: Ontogeny of the human thymus during fetal development. J Clin Immunol 7: 81–97, 1987.PubMedCrossRefGoogle Scholar
  157. 157.
    Colic M, Matanovic D, Hegedis Lj, Dujic A: Immunohistochemical characterization of rat thymic non-lymphoid cells. I. Epithelial and mesenchymal components defined by monoclonal antibodies. Immunology 65: 277–284, 1988.PubMedGoogle Scholar
  158. 158.
    Bloodworth JMB, Hiratsuka H, Hickey RC, Wu J: Ultrastructure of the human thymus, thymic tumors, and myasthenia gravis. In: Pathology Annual. Vol. 10. (Sonners SC, ed) New York, Appleton Century-Crofts, 1975, pp 329–391.Google Scholar
  159. 159.
    Nabarra B, Papiernik M: Phenotype of thymic stromal cells. An immunoelectron microscopic study with anti-IA, anti-MAC-1, and anti-MAC-2 antibodies. Lab Invest 58: 524–531, 1988.PubMedGoogle Scholar
  160. 160.
    Kaiserling E, Stein H, üller-Hermelink HK: Interdigitating reticulum cells in the human thymus. Cell Tissue Res 155: 47–55, 1974.PubMedCrossRefGoogle Scholar
  161. 161.
    Duijvestijn AM, Kohler YG, Hoefsmit ECM: Interdigitating cells and macrophages in the acute involving rat thymus. An electro-microscopic study on phagocytic activity and population development. Cell Tissue Res 224: 291–301, 1982.PubMedCrossRefGoogle Scholar
  162. 162.
    Vicente A, Varas A, Sacedón R, Zapata AG: Histogenesis of the epithelial component of rat thymus: an ultrastructural and immunohistological analysis. Anat Rec 244: 506–519, 1996.PubMedCrossRefGoogle Scholar
  163. 163.
    Bodey B, Bodey B Jr, Kemshead JT, Siegel SE, Kaiser HE: Identification of neural crest derived cells within the cellular microenvironment of the human thymus employing a library of monoclonal antibodies raised against neuronal tissues. IN VIVO 10: 39–47, 1996.PubMedGoogle Scholar
  164. 164.
    Bodey B, Bodey B Jr, Hall FL: Expression of Transforming Growth Factor-β type II receptors in the cells of the human thymic microenvironment during ontogenesis. In: Molecular Biology of Hematopoiesis. Vol. 5. (NG Abraham, S Asano, G Brittinger, R. Shadduck, eds) New York, Plenum Press, Chapter 78, 1996, pp. 645–658.Google Scholar
  165. 165.
    Bodey B, Bodey B Jr, Siegel SE, Kaiser HE: Immunocytochemical Detection of the Homeobox B3, B4, and C6 Gene Products within the Human Thymic Cellular Microenvironment. IN VIVO, 2000; 14: 419–424.PubMedGoogle Scholar
  166. 166.
    Bodey B, Bodey B Jr, Siegel SE, Kaiser HE: Novel Insights into the Function of the Thymic Hassalľs Bodies. IN VIVO, 2000; 14: 407–418.PubMedGoogle Scholar
  167. 167.
    Koka T: Electron-microscopical studies on the thymus, especially on its epithelial cells. Igaku Kenkyu 30: 309–325, 1960; and Excerpta Med 15: 101, 1961.Google Scholar
  168. 168.
    Kohnen P, Weiss L: An electron microscopic study of thymic corpuscles in the guinea pig and the mouse. Anat Rec 148: 29–58, 1964.PubMedCrossRefGoogle Scholar
  169. 169.
    Ito T, Hoshino T: Fine structure of the epithelial reticular cells of the medulla of the thymus in the golden hamster. Z Zellforsch 69: 311–318, 1966.PubMedCrossRefGoogle Scholar
  170. 170.
    Frazier JA: Ultrastructure of the chick thymus. Z Zellforsch 136: 191–205, 1973.PubMedCrossRefGoogle Scholar
  171. 171.
    Mandel T: The development and structure of Hassalľs corpuscles in the guinea pig. A light and electron microscopic study. Z Zellforsch 89: 180–192, 1968.PubMedCrossRefGoogle Scholar
  172. 172.
    Haar JL: Light and electron microscopy of human fetal thymus. Anat Rec 179: 463–476, 1974.PubMedCrossRefGoogle Scholar
  173. 173.
    Hwang WS, Ho TY, Luck SC, Simon GT: Ultrastructure of the rat thymus. Lab Invest 31: 473–487, 1974.PubMedGoogle Scholar
  174. 174.
    Mandel T: Ultrastructure of epithelial cells in the medulla of guinea pig thymus. Austr J Exptl Biol Med Sci 46: 755–767, 1968.Google Scholar
  175. 175.
    Mandel T: Differentiation of epithelial cells in the mouse thymus. Z Zellforsch 106: 498–515, 1970.PubMedCrossRefGoogle Scholar
  176. 176.
    Parakkal PF: An electron microscopic study of esophageal epithelium in the newborn and adult mouse. Am J Anat 121: 175–195, 1967.PubMedCrossRefGoogle Scholar
  177. 177.
    Hirokawa K: Electron microscopic observation of the human thymus of the fetus and the newborn. Acta Pathol Jpn 19: 1–13, 1969.PubMedGoogle Scholar
  178. 178.
    Lavker RM, Matoltsy AG: Substructure of keratohyalin granules of the epidermis as revealed by high resolution electron microscopy. J Ultrastruct Res 35: 575–581, 1971.PubMedCrossRefGoogle Scholar
  179. 179.
    Dearth OA: Late development of the thymus in the cat: nature and significance of the corpuscles of Hassall and cystic formations. Am J Anat 41: 321–345, 1928.CrossRefGoogle Scholar
  180. 180.
    Parakkal PF, Matoltsy AG: An electron microscopic study of developing chick skin. J Ultrastruct Res 23: 403–416, 1968.PubMedCrossRefGoogle Scholar
  181. 181.
    Didierjean L, Saurat JH: Epidermis and thymus. Similar antigenic properties in Hassalľs corpuscle and subsets of keratinocytes. Clin Exp Dermatol 5: 395–404, 1980.PubMedGoogle Scholar
  182. 182.
    Frithiof L, Wersall J: A highly ordered structure in keratinizing human oral epithelium. J Ultrastruct Res 12: 371–379, 1965.PubMedCrossRefGoogle Scholar
  183. 183.
    Kameya T, Watanabe Y: Electronmicroscopic observations on human thymus and thymoma. Acta Pathol Jap 15: 223–246, 1965.Google Scholar
  184. 184.
    Jolly J, Levin S: Sur le modifications histologiques du thymus à la suite de jeûne. C R Soc Biol (Paris) 71: 374–377, 1911.Google Scholar
  185. 185.
    Jackson CA: The effects of inanition and malnutrition upon growth and structure. McGraw-Hill, Philadelphia, 1925.Google Scholar
  186. 186.
    Boyd E: Growth of the thymus. Am J Dis Child 33: 867–879, 1927.Google Scholar
  187. 187.
    Jonson A: Studien über die Thymusinvolution. Die akzidentelle Involution bei Hunger. Arch Mikr Anat 73: 390–443, 1909.Google Scholar
  188. 188.
    Oksanen A: Thymus in acute starvation and re-feeding, an experimental histoquantitative and statistical study in mice. Ann Acad Sci Fenn (A) IV Biologica 181: 1–37, 1971.Google Scholar
  189. 189.
    Henry L: “Accidental” involution of the human thymus. J Pathol Bacteriol 96: 337–343, 1968.PubMedCrossRefGoogle Scholar
  190. 190.
    Regaud C, Crémieu R: Sur ľ involution du thymus produite par les rayons X; resultats experimentaux. Lyon Med 116: 1–18, 1911.Google Scholar
  191. 191.
    Malkina DG: Changes in Hassal’s corpuscles during thymus regeneration in the condition of roentgen irradiation. Biull Eksp Biol Med 52: 104–109, 1961.PubMedGoogle Scholar
  192. 192.
    Jaroslow BN: Genesis of Hassal’s corpuscles. Nature 215: 408–409, 1967.PubMedGoogle Scholar
  193. 193.
    Maor D, Alexander P: Abscopal stimulation of the thymus of rats by exposure of the head to x-rays. Nature 205: 40–42, 1965.PubMedGoogle Scholar
  194. 194.
    Maor D, Alexander P: Possible role of growth hormone in the stimulation of the thymus of rats following irradiation of the head. Experientia 28: 957–959, 1972.PubMedCrossRefGoogle Scholar
  195. 195.
    Calvo W, Fliedner TM, Herbst EW, Hügl E, Bodey B: Degenerative changes and recovery of the thymus of lethally irradiated dogs, rescued by transfusion of cryopreserved autologous blood leukocytes. Exp Hematol 15: 1171–1178, 1987.PubMedGoogle Scholar
  196. 196.
    Ross MA, Korenchevsky V: Thymus of rat and sex hormones. J Pathol Bacteriol 52: 349–360, 1941.CrossRefGoogle Scholar
  197. 197.
    Plagge JC: Some effects of prolonged massive estrogen treatment on rat, with special reference to thymus. Arch Pathol 42: 598–606, 1946.Google Scholar
  198. 198.
    Hume EM, Burbank R, Korenchevsky V: Some effects of administration of oestrogens on organs of castrated and non-castrated male rats partially deprived of vitamin A. J Pathol Bacteriol 49: 291–298, 1939.CrossRefGoogle Scholar
  199. 199.
    Korenchevsky V, Hall K: Pathological changes in sex organs after prolonged administration of sex hormones to female rats. J Pathol Bacteriol 50: 295–315, 1940.CrossRefGoogle Scholar
  200. 200.
    Gitlin D, Landing BH, Whipple A: Localization of homologous plasma proteins in tissues of young human beings as demonstrated with fluorescent antibodies. J Exp Med 97: 163–176, 1953.CrossRefGoogle Scholar
  201. 201.
    Kouvalainen K: Significance of Hassal’s corpuscles in the light of their morphological and histochemical appearance. Ann Med Exp Fenn 42: 177–184, 1964.PubMedGoogle Scholar
  202. 202.
    Burnet FM: The clonal selection theory of acquired immunity. Nashville, Vanderbilt Univ Press, 1959.Google Scholar
  203. 203.
    Burnet FM: Immunological recognition of self. Science 133: 307–311, 1961.PubMedGoogle Scholar
  204. 204.
    Burnet FM: The immunological significance of the thymus: an extension of the clonal selection theory of immunity. Aust Ann Med 11: 19–91, 1962.Google Scholar
  205. 205.
    Burnet FM: Role of the thymus and related organs in immunity. Br Med J 2: 807–811, 1962.PubMedGoogle Scholar
  206. 206.
    Blau JN: Antigen and antibody localization in Hassal’s corpuscles. Nature 215: 1073–1075, 1967.PubMedGoogle Scholar
  207. 207.
    Blau JN: The dynamic behavior of Hassal’s corpuscles and the transport of particulate matter in the thymus of the guinea-pig. Immunology 13: 281–292, 1967.PubMedGoogle Scholar
  208. 208.
    Blau JN: The localization of BCG in the guinea-pig thymus with special reference to Hassal’s corpuscles. Immunology 17: 995–998, 1969.PubMedGoogle Scholar
  209. 209.
    Kater L, Gorp LH van: Morphological aspects of Hassal’s corpuscles. Path Eur 4: 361–369, 1969.Google Scholar
  210. 210.
    Tomasi TB Jr, Yurchak AM: The synthesis of secretory component by the human thymus. J Immunol 108: 1132–1135, 1972.PubMedGoogle Scholar
  211. 211.
    Blau JN: A phagocytic function of Hassal’s corpuscles. Nature 208: 564–566, 1965.Google Scholar
  212. 212.
    Blau JN: Histological changes and macrophage activity in the adult guinea-pig thymus. Br J Exp Pathol 52: 142–146, 1971.PubMedGoogle Scholar
  213. 213.
    Wassen A: Beobachtungen an Thymuskulturen in vitro. Anat Hefte 52: 279–318, 1914.Google Scholar
  214. 214.
    Tschassownikow N: Übery die in vitro-Kulturen des Thymus. Arch Exp Zellforsch 3: 250–276, 1926.Google Scholar
  215. 215.
    Tschassownikow N: Einige Daten zur Frage von der Struktur der Thymusdrüse auf Grund experimenteller Forschungen (Gewebszüchtung in vitro, Röntgenbestrahlung, Vitalfärbung mit Lithiokarmin). Ztschr Zellforsch 8: 251–295, 1929.Google Scholar
  216. 216.
    Murray RG: Pure cultures of rabbit thymus epithelium. Am J Anat 81: 369–411, 1947.CrossRefPubMedGoogle Scholar
  217. 217.
    Mandel T, Russell PJ: Differentiation of foetal mouse thymus. Ultrastructure of organ cultures and of subcapsular grafts. Immunology 21: 659–674, 1971.PubMedGoogle Scholar
  218. 218.
    Mandel T, Russell PJ, Byrd W: Differentiation of the thymus in vivo and in vitro. In: Cell Interactions. Proceedings of the Third Lepetit Colloquium (Silvestri LG, North Holland, eds) Amsterdam, 1972, pp 183–191.Google Scholar
  219. 219.
    Pyke KW, Gelfand EW: Morphological and functional maturation of human thymic epithelium in culture. Nature 251: 421–423, 1974.PubMedCrossRefGoogle Scholar
  220. 220.
    Papiernik M, Nabarra B, Bach JF: In vitro culture of functional human thymic epithelium. Clin Exp Immunol 19: 281–287, 1975.PubMedGoogle Scholar
  221. 221.
    Kruisbeek AM: Thymic factors and T-cell maturation in vitro: a comparison of the effects of thymic epithelial cultures with thymic extracts and thymus dependent serum factors. Thymus 1: 163–185, 1979.PubMedGoogle Scholar
  222. 222.
    Kruisbeek AM: Effect of factors in thymic epithelial culture supernatants (TES) on in vitro maturation of thymocytes. Ann NY Acad Sci 332: 109–112, 1979.PubMedGoogle Scholar
  223. 223.
    Ito T, Kasahara S, Aizu S, Kato K, Takeuchi M, Mori T: Formation of Hassal’s corpuscles in vitro by the thymic epithelial cell line IT-26R 21 of the rat. Cell Tissue Res 226: 469–476, 1982.Google Scholar
  224. 224.
    Jonse KH, St. Pierre RL: Analysis of cellular heterogeneity in mouse thymus cultures. In Vitro 17: 431–440, 1981.Google Scholar
  225. 225.
    Singer KH, Harden EA, Robertson AL, Lobach DF, Haynes BF: In vitro growth and phenotypic characterization of mesodermal-derived and epithelial components of normal and abnormal human thymus. Human Immunol 13: 161–176, 1985.Google Scholar
  226. 226.
    Hammar JA: Über Bakterientoxine als Anreger einer Neubildung Hassallscher Körper. Z Mikrosk Anat Forsch 9: 68–78, 1927.Google Scholar
  227. 227.
    Kouvalainen K: Immunologic features in the congenital nephrotic syndrome: a clinical and experimental study. Ann Paediat Fenn 9 (Suppl 122): 1–137, 1963.Google Scholar
  228. 228.
    Sloan HE: The thymus in myasthenia gravis with observations on the normal anatomy and histology of the thymus. Surgery 13: 154–174, 1943.Google Scholar
  229. 229.
    Wilson A, Wilson H: Thymus and myasthenia gravis. Am J Med 19: 697–700, 1955.PubMedGoogle Scholar
  230. 230.
    Miller JFAP: Effect of neonatal thymectomy on the immunological response of the mouse. Proc Roy Soc Biol Sci 156: 415–428, 1962.Google Scholar
  231. 231.
    White RG, Marshall AHE: The autoimmune response in myasthenia gravis. Lancet II: 120–123, 1962.Google Scholar
  232. 232.
    Goldstein G: Thymitis and myasthenia. Lancet II: 1164–1167, 1966.Google Scholar
  233. 233.
    Thurner K: Über den Einfluss des Thymus Extrakten auf die Leistungsfähigkeit und Ermüdbarkeit des Säugetiermuskels. Arch Ges Physiol 202: 444–467, 1924.CrossRefGoogle Scholar
  234. 234.
    Torda C, Wolff HG: Effect of organ extracts and their fractions on acetylcholine synthesis. Am J Physiol 148: 417–423, 1947.PubMedGoogle Scholar
  235. 235.
    Parkes JD, McKinna JA: Effects of thymic extract on the neuromuscular junction. Nature 214: 1116–1117, 1967.PubMedGoogle Scholar
  236. 236.
    Goldstein G: The thymus and neuromuscular function. Lancet II: 119–123, 1968.Google Scholar
  237. 237.
    Trainin N, Small M: Thymic humoral factors. In: Contemporary Topics in Immunobiology. Vol. 2. (Carter RL, Davies AJS, eds) New York, Plenum Press, 1973, pp 321–337.Google Scholar
  238. 238.
    Raviola E, Raviola G: Striated muscle cells in the thymus of reptiles and birds: an electron microscopic study. Am J Anat 121: 623–646, 1967.PubMedCrossRefGoogle Scholar
  239. 239.
    Zoltowska A: Myoid and epithelial cell differentiation in myasthenic thymuses. Thymus 17: 237–248, 1991.PubMedGoogle Scholar
  240. 240.
    Hsiao L, Takahashi K, Takeya M, Arao T: Differentiation and maturation of macrophages into interdigitating cells and their multicellular complex formation in fetal and postnatal rat thymus. Thymus, 17: 219–235, 1991.PubMedGoogle Scholar
  241. 241.
    Ardavin C, Wu L, Li Ch, Shortman K: Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population. Nature 362: 761–763, 1993.PubMedCrossRefGoogle Scholar
  242. 242.
    Inaba K, Inaba M, Deguchi M, Hagi K, Yasumizu R, Ikehara S, Muramatsu S, Steinman R: Granulocytes, macrophages, and dendritic cells arise from a common major histocompatibility complex class II-negative progenitor in mouse bone marrow. Proc Natl Acad Sci USA 90: 3038–3042, 1993.PubMedGoogle Scholar
  243. 243.
    Arkema A: Dendritic cells: ultrastructure and function. Doctoral Thesis, Free University, Amsterdam, The Netherlands, 1992.Google Scholar
  244. 244.
    Burnet FM, Mackay IR: Lymphoepithelial structures and autoimmune disease Lancet II: 1030–1033, 1962.Google Scholar
  245. 245.
    Waldeyer W: Die Rückbildung des Thymus. Sitzungsber Akad Wissensch 25: 433–446, 1890.Google Scholar
  246. 246.
    Syk I: Ueber Altersveränderungen in der Anzahl der Hassallschen Körper, nebst einem Beitrag zum Studium der Mengenverhältnisse der Mitosen in dem Kaninchenthymus. Anat Anz 34: 560–567, 1909.Google Scholar
  247. 247.
    Oosterom R, Kater L: The thymus in the aging individual. II. Thymic epithelial function in vitro in aging and in thymus pathology. Clin Immunol Immunopathol 18: 195–202, 1981.PubMedGoogle Scholar
  248. 248.
    Müller-Hermelink HK, Steinmann GG: Age-related alterations of intrathymic microenvironment. In: Lymphoid cell functions in aging. Eurage Series No. III. (de Weck AL, ed) 1984, pp 75–82.Google Scholar
  249. 249.
    Steinmann GG: Changes of the human thymus during aging. In: The Human Thymus. Histophysiology and Pathology. Current Topics in Pathology. Vol. 75. (Müller-Hermelink HK, ed) Berlin-Heidelberg-New York-Tokyo, Springer, 1986, pp 43–88.Google Scholar
  250. 250.
    Good RA, Gabrielsen AE: The thymus in immunobiology. Structure, function and role in disease. New York, Harper & Row, Inc., 1964.Google Scholar
  251. 251.
    Hammar JA: Beiträge zur Konstitutionsanatomie. 1. Mikroskopische Analyse des Thymus in 25 Fällen Basedowscher Krankheit. Beitr Klin Chir 104: 469–614, 1917.Google Scholar
  252. 252.
    Hammar JA: Die Menschenthymus in Gesundheit und Krankheit. Teil II. Das Organ unter anormalen Körperverhältnissen. Zugleich Grundlagen det Theorie der Thymusfunktion. Z Mikr Anat Forsch (Leipzig) 16, 1929.Google Scholar
  253. 253.
    Marshall AH, White RG: Experimental thymic lesions resembling those of myasthenia gravis. Lancet I: 1030–1031, 1961.Google Scholar
  254. 254.
    Good RA: Aγglobulinaemia — a provocative experiment of nature. Bull Univ Minn Hosp 26: 1–19, 1954.Google Scholar
  255. 255.
    Hitzig WH, Landolt R, Muller G, Bodmer P: Heterogeneity of phenotypic expression in a family with Swiss type aγglobulinemia: observations on the acquisition of aγglobulinemia. J Pediatr 78: 968–980, 1971.PubMedGoogle Scholar
  256. 254.
    Joshi VV, Oleske JM: Pathological appraisal of the thymus gland in acquired immunodeficiency syndrome in children. A study of four cases and a review of the literature. Arch Pathol Lab Med 109: 142–246, 1985.PubMedGoogle Scholar
  257. 255.
    Suster S, Moran CA: Malignant thymic neoplasms that may mimic benign conditions. Semin Diagn Pathol 12: 98–104, 1995.PubMedGoogle Scholar
  258. 256.
    Rosai J, Levine GD: Tumors of the thymus. In: Atlas of Tumor Pathology, 2nd Series, Fascicle 13. Washington: Armed Forces Institute of Pathology, 1976Google Scholar

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