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Biological Molecule Conformations Probed and Enhanced by Metal and Carbon Nanostructures: SEIRA, AFM and SPR Data

  • G. I. Dovbeshko
  • O. P. Paschuk
  • O. M. Fesenko
  • V. I. Chegel
  • Yu. M. Shirshov
  • A. A. Nazarova
  • D. V. Kosenkov
Conference paper
Part of the NATO Science Series II: Mathematics, Physics and Chemistry book series (NAII, volume 152)

Abstract

The use of the method of enhancement of infrared absorption by rough metallic surface (surface enhanced infrared absorption — SEIRA) allows one to increase the probability of infrared transitions and to reveal a series of spectral manifestations of structural features of biological molecules. We analyse various experimental techniques that give the possibility to achieve enhancement in the infrared (IR) spectra. We studied the applicability of the SEIRA techniques for conformational analysis of nucleic acids and proteins (bovine serum albumin — BSA) on gold substrate of 200–500 Å thickness. Under the conditions of our experiment and according to literature data, there was observed enhancement factor equalled to 3...20 for vibrations of various molecular groups. Concentration of BSA solution and thickness of the protein film on gold substrate influence the conformational composition. Conformational state of protein in solution plays a key role after its precipitation on gold substrate. Different roughness of gold surface leads to changes in enhancement factor. Peculiarity of another optical amplifier, namely, colloidal gold that effectively used for enhancement of signal in IR absorption and Raman scattering, have been studied. The structural features of DNA — colloidal gold and BSA — colloidal gold system that obtained in SEIRA and SPR (surface plasmon resonance) experiment are discussed. Atom forth microscopy (AFM) technique was applied to test the roughness of the metal surface. The IR experimental evidence for enhancement of the vibrations of residual graphite on the surface of carbon nanotubes is presented. We made an attempt to model the factor of enhancement of electrical field and their frequency dependence for different metal surfaces and obtained that silver, gold and copper are the best.

Key words

DNA bovine serum albumin (BSA) Fourier transformed infrared spectroscopy (FTIR) surface enhanced infrared absorption (SEIRA) gold substrate gold-colloidal particles surface plasmon resonance (SPR) atom forth microscopy (AFM) 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • G. I. Dovbeshko
    • 1
  • O. P. Paschuk
    • 1
  • O. M. Fesenko
    • 1
  • V. I. Chegel
    • 2
  • Yu. M. Shirshov
    • 2
  • A. A. Nazarova
    • 3
  • D. V. Kosenkov
    • 4
  1. 1.Institute of Physics of National Academy of Sciences of UkraineKyivUkraine
  2. 2.Institute of Semiconductor Physics of National Academy of Sciences of UkraineKyivUkraine
  3. 3.Biological DepartmentNational Taras Shevchenko University of KyivKyivUkraine
  4. 4.Radiophysics DepartmentNational Taras Shevshenko University of KyivKyivUkraine

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