CRALBP Ligand and Protein Interactions

  • Zhiping Wu
  • Sanjoy K. Bhattacharya
  • Zhaoyan Jin
  • Vera L. Bonilha
  • Tianyun Liu
  • Maria Nawrot
  • David C. Teller
  • John C. Saari
  • John W. Crabb
Conference paper

DOI: 10.1007/0-387-32442-9_66

Part of the Advances in Experimental Medicine and Biology book series (volume 572)
Cite this paper as:
Wu Z. et al. (2006) CRALBP Ligand and Protein Interactions. In: Hollyfield J.G., Anderson R.E., LaVail M.M. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 572. Springer, Boston, MA

Abstract

The visual cycle is the complex enzymatic retinoid-processing involved in regenerating bleached rod and cone visual pigments.1 Central to visual cycle physiology is the cellular retinaldehyde-binding protein (CRALBP), a 36kDa cytosolic protein with high affinity for 11-cis-retinal and 11-cis-retinol. CRALBP is expressed in retinal pigment epithelium (RPE) and Müller cells, as well as in ciliary epithelium, iris, cornea, pineal gland and a subset of oligodendrocytes of the optic nerve and brain.2 Its function outside the RPE is not known, although a recent behavioral genetic study suggests that CRALBP may contribute to ethanol preference in mice.3 In the RPE, CRALBP serves as an 11-cis-retinol acceptor in the visual cycle isomerization step and as a substrate carrier for 11-cis-retinol dehydrogenase. 4, 5, 6, 7, 8 These functions require the rapid association and release of retinoid from the CRALBP ligand-binding pocket and involve critical protein interactions. To better understand the visual cycle, we are characterizing CRALBP ligand and protein interactions and retinoid trafficking within the RPE.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Zhiping Wu
    • 1
  • Sanjoy K. Bhattacharya
    • 1
  • Zhaoyan Jin
    • 1
  • Vera L. Bonilha
    • 1
  • Tianyun Liu
    • 2
  • Maria Nawrot
    • 3
  • David C. Teller
    • 2
  • John C. Saari
    • 2
    • 3
  • John W. Crabb
    • 1
  1. 1.Cole Eye InstituteCleveland Clinic FoundationClevelandUSA
  2. 2.Department of BiochemistryUniversity of WashingtonSeattleUSA
  3. 3.Department of OphthalmologyUniversity of WashingtonSeattleUSA

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