Fast Screening of Single-Nucleotide Polymorphisms Using Chip-Based Temperature Gradient Capillary Electrophoresis

  • Peng Liu
  • Wan-Li Xing
  • Dong Liang
  • Guo-Liang Huang
  • Jing Cheng

Abstract

Recently, the analysis of single-nucleotide polymorphisms has attracted much attention. Although many techniques have been reported, new methods with high resolving power, low-cost and fast speed are still in demand. We present a fast SNP detection scheme using chip-based temperature gradient capillary electrophoresis to separate the homoduplex and heteroduplex PCR products which contain one or two SNP sites. The total time of a single run was only 8 minutes.

Key words

Single-nucleotide polymorphisms temperature gradient capillary electrophoresis chip 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Peng Liu
    • 1
    • 2
  • Wan-Li Xing
    • 1
    • 2
  • Dong Liang
    • 1
    • 2
  • Guo-Liang Huang
    • 1
    • 2
  • Jing Cheng
    • 1
    • 2
  1. 1.National Engineering Research Center for Beijing Biochip TechnologyBeijingP. R. China
  2. 2.Department of Biological Sciences & BiotechnologyTsinghua UniversityBeijingP. R. China

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