Skip to main content

Protein Content Quantification by Bradford Method

  • Chapter
Handbook of Plant Ecophysiology Techniques

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aerts R. Interespecific competition in natural plant communities: mechanisms, trade-offs and plant-soil feedbacks. J Exp Bot 1999; 50:29–37

    Article  CAS  Google Scholar 

  • Amthor J.S., McCree K.J. “Carbon Balance of Stressed Plants: A Conceptual Model for Integrating Research Results.” In Stress Responses in Plants: Adaptation and Acclimation Mechanisms. R.G. Alscher, J.R. Cumming, eds. New York: Wiley-Liss Inc. Publishers, 1990

    Google Scholar 

  • Arulsekar S., Parfitt D.E. Isozyme analysis procedures for stone fruits, almond, grape, walnut, pistachio, and fig. Hortscience 1986; 21:928–933

    CAS  Google Scholar 

  • Ashraf M., Yasmin N. Responses of 4 arid zone grass species from varying habitats to drought stress. Biol Plantarum 1995; 37:567–575

    Google Scholar 

  • Austin M.P. “Community Theory and Competition in Vegetation.” In Perspectives on Plant Competition. J.B. Grace, D. Tilman, eds. San Diego: Academic Press, 1990

    Google Scholar 

  • Berges J.A., Fisher A.E., Harrison P.J. A comparison of Lowry, Bradford and Smith protein assays using different protein standards and protein isolated from the marine diatom Thalassiosira pseudonana. Mar Biol 1993; 115:187–193

    Article  CAS  Google Scholar 

  • Bohnert H.J., Jensen R.G. Metabolic engineering for increased salt tolerance — the next step. Aust J Plant Physiol 1996; 23:661–667

    Article  Google Scholar 

  • Boyer R.F. Modern Experimental Biochemistry. Massachusetts: Addison-Wesley Publishing Co., 1986

    Google Scholar 

  • Bradford M.M. A rapid sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-Dye Binding. Anal Biochem 1976; 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Bray E.A. Molecular responses to water deficit. Plant Physiol 1993; 103:1035–1040

    PubMed  CAS  Google Scholar 

  • Chapin III F.S. The mineral nutrition of wild plants. Annu Rev Ecol Syst 1980; 11:233–260

    Article  CAS  Google Scholar 

  • Chapin III F.S., Bloom A.J., Field C.B., Waring R.H. Plant responses to multiple environmental factors. BioScience 1987; 37:49–57

    Google Scholar 

  • Chapin III F.S. Integrated responses of plants to stress. BioScience 1991; 41:29–36

    Google Scholar 

  • Compton S.J., Jones C.G. Mechanisms of dye response and interference in the Bradford protein assay. Anal Biochem 1985; 151:369–374

    Article  PubMed  CAS  Google Scholar 

  • Conroy J.P., Virgona J.M., Smillie R.M., Barlow E.W. Influence of drought acclimation and CO 2 enrichment on osmotic adjustment and chlorophyll a fluorescence of sunflower during drought. Plant Physiol 1988; 86:1108–1115

    Article  CAS  PubMed  Google Scholar 

  • Dunson W.A., Travis J. The role of abiotic factors in community organization. Am Nat 1991; 138:1067–1091

    Article  Google Scholar 

  • Gan S., Amasino R.M. Making sense of senescence. Plant Physiol 1997; 113:313–319

    PubMed  CAS  Google Scholar 

  • Hsiao T.C. Plant responses to water stress. Annu Rev Plant Physiol 1973; 24:519–570

    Article  CAS  Google Scholar 

  • Lambers H., Poorter H. Inherent variation in growth rate between higher plants: a search for physiological causes and ecological consequences. Adv Ecol Res 1992; 23:187–261

    CAS  Google Scholar 

  • Lambers H., Chapin III F.S., Pons T.L. Plant Physiological Ecology. New York: Springer-Verlang, 1998

    Google Scholar 

  • Larcher W. Physiological Plant Ecology. Berlin: Springer-Verlag, 1995

    Google Scholar 

  • Lemaire G., Millard P. An ecophysiological approach to modelling resource fluxes in competing plants. J Exp Bot 1999; 50:15–28

    Article  CAS  Google Scholar 

  • Lichtenthaler H.K. Vegetation stress: An introduction to the stress concept in plants. J Plant Physiol 1996; 148:4–14

    CAS  Google Scholar 

  • Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with Folin-phenol reagent. J Biol Chem 1951; 193:265–275

    PubMed  CAS  Google Scholar 

  • Millard P. The accumulation and storage of nitrogen by herbaceous plants. Plant Cell Environ 1988; 11:1–8

    CAS  Google Scholar 

  • Pankovic D., Sakac Z., Kevresan S., Plesnicar M. Acclimation to long-term water deficit in the leaves of two sunflower hybrids: photosynthesis, electron transport and carbon metabolism. J Exp Bot 1999; 50:127–138

    Article  CAS  Google Scholar 

  • Pedrol N. “Analítica de proteínas totales por colorimetría.” In Manual de Técnicas en Ecofisiología Vegetal. N. Pedrol, M.J. Reigosa, eds. Vigo, Spain: Gamesal, 1997

    Google Scholar 

  • Pedrol N., Ramos P., Reigosa M.J. Phenotypic plasticity and acclimation to water deficits in velvet-grass: a long-term greenhouse experiment. Changes in leaf morphology, photosynthesis and stress-induced metabolites. J Plant Physiol 2000; 157:383–393

    CAS  Google Scholar 

  • Pell E.J., Dann M.S. “Multiple Stress-induced Foliar Senescence and Implications for Wholeplant Longevity.” In Response of Plants to Multiple Stresses. H. A. Mooney, W.E. Winner, E.J. Pell, eds. San Diego: Academic Press, 1991

    Google Scholar 

  • Setter T.L. “Transport/Harvest Index: Photosyntate Partitioning in Stressed Plants.” In Stress Responses in Plants: Adaptation and Acclimation Mechanisms. R.G. Alscher, J.R. Cumming, eds. New York: Wiley-Liss Inc., 1990

    Google Scholar 

  • Shangguan Z., Shao M., Dyckmans J. Interaction of osmotic adjustment and photosynthesis in winter wheat under soil drought. J Plant Physiol 1999; 154:753–758

    CAS  Google Scholar 

  • Snyder J.C., Desborough S.L. Rapid estimation of potato tuber total protein content with Coomassie brillant blue G-250. Theor Appl Genet 1978; 52:135–139

    Article  CAS  Google Scholar 

  • Stitt M., Schulze D. Does Rubisco control the rate of photosynthesis and plant growth? An exercise in molecular ecophysiology. Plant Cell Environ 1994; 17:465–487

    CAS  Google Scholar 

  • Wedin D.A. “Species, Nitrogen and Grassland Dynamics: the Constraints of Stuff.” In Linking Species and Ecosystems. C. Jones, J.H. Lawton, eds. New York: Chapman and Hall, 1994

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Manuel J. Reigosa Roger

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Kluwer Academic Publishers

About this chapter

Cite this chapter

Bonjoch, N.P., Tamayo, P.R. (2001). Protein Content Quantification by Bradford Method. In: Reigosa Roger, M.J. (eds) Handbook of Plant Ecophysiology Techniques. Springer, Dordrecht. https://doi.org/10.1007/0-306-48057-3_19

Download citation

  • DOI: https://doi.org/10.1007/0-306-48057-3_19

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-7053-6

  • Online ISBN: 978-0-306-48057-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics