Plant Nutrition pp 132-133 | Cite as
Regulation of the accumulation of β-Subunit of β-Conglycinin in nitrogen starved soybean (Glycine max L.) seed by amino acids and organic acids
Abstract
It has been reported that the accumulation of soybean (Glycine max L.) storage protein, the β-subunit of β-conglycinin, was suppressed by nitrogen (N)-deficiency. In this report we tried to evaluate what compound(s) may play a key role in regulating the accumulation of β-subunit mRNA in vitro cotyledon culture system. The cotyledons from non-nodulating soybean plants cultivated under N-deficient condition (0.5 mM NO3−) were transferred to an in vitro culture system. The application of glutamine (Gln) enhanced β-subunit mRNA accumulation within twelve hours. The application of glutamic acid, alanine, or aspartic acid could not induce the ß-subunit mRNA accumulation. Either azaserine or (aminooxy)acetic acid, an inhibitor of transaminase, inhibited the β-subunit mRNA accumulation when Gln was the sole nitrogen source. In these cotyledons, Gln was accumulated at a high level. Application of citrate could enhance the accumulation of β-subunit mRNA in a N free culture medium. These results suggested that Gln might not be a direct signal to enhance β-subunit mRNA accumulation. The β-subunit mRNA accumulation appeared to be induced by organic acid(s).
Key words
amino acid β-conglycinin β-subunit nitrogen starved organic acids soybean seedReferences
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