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Opiates Promote T Cell Apoptosis Through JNK and Caspase Pathway

  • Pravin Singhal
  • Aditi Kapasi
  • Krishna Reddy
  • Nicholas Franki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 493)

Abstract

Opiate addicts are prone to recurrent infections. In the present study we evaluated the molecular mechanism of opiate-induced T cell apoptosis. Both morphine and DAGO ([D-Ala2,N-Me-Phe4,Gly5-ol]enkephalin) enhanced T cell apoptosis. Morphine as well as DAGO activated c-Jun NH 2 -terminal kinase (JNK) in T cells. Moreover, opiates increased the expression of ATF-2, a specific substrate for JNK and P38 mitogen activated kinases (MAPK). Furthermore, opiates attenuated extracellular signal related kinase (ERK) in T cells. Both morphine and DAGO cleaved pro-caspases 8, 9, and 10 and generated caspases 8, 9 and 10 (active products). Morphine as well as DAGO also cleaved poly- (ADP-ribose) polymerase (PARP) into 116 and 85 kD proteins indicating the activation of caspase-3. These results suggest that opiate-induced T cell apoptosis may be mediated through the JNK cascade and activation of caspases 8 and 3.

Keywords

Cell Apoptosis Jurkat Cell Lymphocyte Apoptosis Extracellular Signal Related Kinase Opiate Addict 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Pravin Singhal
    • 1
  • Aditi Kapasi
    • 1
  • Krishna Reddy
    • 1
  • Nicholas Franki
    • 1
  1. 1.Molecular Biology and Experimental Pathology Division of Kidney Diseases and HypertensionLong Island Jewish Medical CenterNew Hyde Park

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