Isolation and Cultivation of Osteoclasts and Osteoclast-Like Cells

Kanis JA (1995) Bone and cancer: pathophysiology and treatment of metastases. Bone 17: 101S–105S.CrossRefPubMedGoogle Scholar

Taube T, Elomaa I, Blomqvist C, Beneton MN, and Kanis JA (1994) Histomorphometric evidence for osteoclast-mediated bone resorption in metastatic breast cancer. Bone 15: 161–6.CrossRefPubMedGoogle Scholar

Mundy GR (1993) Cytokines and growth factors in the regulation of bone remodeling. J Bone Miner Res 8:S505–10.CrossRefGoogle Scholar

Suda T, Udagawa N, Nakamura I, Miyaura C, and Takahashi N (1995) Modulation of osteoclast differentiation by local factors. Bone 17: S87–S91.CrossRefGoogle Scholar

Wiebe SH, Hafezi M, Sandhu HS, Sims SM, and Dixon SJ (1996) Osteoclast activation in inflammatory periodontal diseases. Oral Diseases 2: 167–80.PubMedGoogle Scholar

Manolagas SC, Bellido T, and Jilka RL (1995) New insights into the cellular, biochemical, and molecular basis of postmenopausal and senile osteoporosis: Roles of IL-6 and gp130. Intl J Immunopharmacol 17:109–16.CrossRefGoogle Scholar

MacDonald BR, Takahashi N, McManus LM, Holahan J, Mundy GR, and Roodman GD (1987) Formation of multinucleated cells that respond to osteotropic hormones in long term human BM cultures. Endocrinol 120: 2326–33.CrossRefGoogle Scholar

Helfrich MH, Thesingh CW, Mieremet RH, and van Iperen-van Gent AS (1987) Osteoclast generation from human fetal BM in cocultures with murine fetal long bones. A model for in vitro study of human osteoclast formation and function. Cell Tiss Res 249: 125–36.CrossRefGoogle Scholar

Roodman GD (1996) Advances in bone biology — The osteoclast. Endocrine Revs 17: 308–332.CrossRefGoogle Scholar

Yoneda T, Alsina MM, Garcia JL, and Mundy GR (1991) Differentiation of HL-60 cells into cells with the osteoclast phenotype. Endocrinol 129: 683–9.Google Scholar

Somerman MJ, Berry JE, Khalkhali-Ellis Z, Osdoby P, and Simpson RU (1995) Enhanced expression of alpha v integrin subunit and osteopontin during differentiation of HL-60 cells along the monocytic pathway. Exp Cell Res 216: 335–41.CrossRefPubMedGoogle Scholar

Gattei V, Bernabei PA, Pinto A, Bezzini R, Ringressi A, Formigli L, Tanini A, Attadia V, and Brandi ML (1992) Phorbol ester induced osteoclast-like differentiation of a novel human leukemic cell line (FLG 29.1). J Cell Biol 116: 437–47.CrossRefPubMedGoogle Scholar

Khalkhali-Ellis Z, Collin-Osdoby P, Li L, Brandi ML, and Osdoby P (1997) A human homolog of the 150 kD avian osteoclast membrane antigen related to superoxide dismutase and essential for bone resorption is induced by developmental agents and opposed by estrogen in FLG 29.1 cells. Calcif Tiss Intl 60:187–93.CrossRefGoogle Scholar

Boyde A and Jones SJ (1991) Pitfalls in pit measurement. Calcif Tiss Intl 49: 65–70.CrossRefGoogle Scholar

Minkin C (1982) Bone acid phosphatase: tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif Tiss Intl 34: 285–90.CrossRefGoogle Scholar

Littlewoodevans A, Kokubo T, Ishibashi O, Inaoka T, Wlodarski B, Gallagher JA, and Bilbe G (1997) Localization of cathepsin K in human osteoclasts by in situ hybridization and immunohistochemistry. Bone 20: 81–86.CrossRefGoogle Scholar

Horton MA, Dorey EL, Nesbitt SA, Samanen J, Ali FE, Stadel JM, Nichols A, Greig R, and Helfrich MH (1993) Modulation of vitronectin receptor-mediated osteoclast adhesion by Arg-Gly-Asp peptide analogs: a structure-function analysis. J Bone Mirier Res 8: 239–47.Google Scholar

Rathod H, Malcolm AJ, Gillespie JI, Berry V, Pooley J, Piggott NH, and Datta HK (1994) Characterization of a subtype of primary osteoclastoma: extracellular calcium but not calcitonin inhibits aggressive HLA-DR-positive osteoclastoma possessing ‘functional’ calcitonin receptors. J Pathol 174: 293–9.CrossRefPubMedGoogle Scholar

Zheng MH, Fan Y. Wysocki S, Wood DJ, and Papadimitriou JM (1993) Detection of mRNA for carbonic anhydrase II in human osteoclast-like cells by in situ hybridization. J Bone Miner Res 8: 113–8.PubMedGoogle Scholar

Chatterjee D, Chakraborty M, Leit M, Neff L, Jamsa-Kellokumpu S, Fuchs R, Bartkiewicz M, Hernando N, and Baron R (1992) The osteoclast proton pump differs in its pharmacology and catalytic subunits from other vacuolar H(+)-ATPases. J Exp Biol 172: 193–204.PubMedGoogle Scholar

Oursler MJ. Li L, and Osdoby P (1991) Purification and characterization of an osteoclast membrane glycoprotein with homology to manganese superoxide dismutase. J Cell Biochem 46: 219–33.PubMedCrossRefGoogle Scholar

Gay CV (1996) Role of microscopy in elucidating the mechanism and regulation of the osteoclast resorptive apparatus. Microsc Res Tech 33: 165–70.CrossRefPubMedGoogle Scholar

Fuller K and Chambers T (1989) Bone matrix stimulates osteoclastic differentiation in cultures of rabbit BM cells. J Bone Miner Res 4: 179–183.PubMedGoogle Scholar

Collin-Osdoby P, Oursler M, Rothe L, Webber D, Anderson F, and Osdoby P (1995) Osteoclast 12 IF antigen expression during osteoblast conditioned medium induction of ostcoclast-like cells in vitro: Relationship to calcitonin responsiveness, tartrate resistant acid phosphatase levels, and bone resorptive activity. J Bone Miner Res 10: 45–58.PubMedGoogle Scholar

Akatsu T, Tamura T, Takahashi N, Udagawa N, Tanaka S, Sasaki T, Yamaguchi A, Nagata N, and Suda T (1992) Preparation and characterization of a mouse osteoclast-like multinucleated cell population. J Bone Miner Res 7: 1297–306.PubMedGoogle Scholar

Galvin RJ, Bryan P, Horn JW, Rippy MK, and Thomas JE (1996) Development and characterization of a porcine model to study osteoclast differentiation and activity. Bone 19: 271–9.PubMedCrossRefGoogle Scholar

Kukita A, Kukita T, Shin J, and Kohashi O (1993) Induction of mononuclear precursor cells with osteoclastic phenotypes in a rat BM culture system depleted of stromal cells. Biochem Biophys Res Commun 196: 1383–1389.CrossRefPubMedGoogle Scholar

Flanagan AM, Horton MA, Dorey EL, Collins DA, Evely RS, Moseley JM, Firkin FC, Chambers TJ, Helfrich MH, and Martin TJ (1992) An assessment of the ability of human BM cultures to generate osteoclasts. Intl J Exptl Pathol 73: 387–401.Google Scholar

Demuldcr A, Suggs SV, Zsebo KM, Scarcez T, and Roodman GD (1992) Effects of stem cell factor on osteoclast-like cell formation in long-term human marrow cultures. J Bone Miner Res 7: 1337–44.Google Scholar

Roux S, Quinn J, Pichaud F, Orcel P, Chastre E, Jullienne A, and De Vernejoul MC (1996) Human cord blood monocytes undergo terminal osteoclast differentiation in vitro in the presence of culture medium conditioned by giant cell tumor of bone. J Cell Physiol 168: 489–98.CrossRefPubMedGoogle Scholar

Connor JR, Dodds RA, James IE, and Gowen M (1995) Human osteoclast and giant cell differentiation: the apparent switch from nonspecific esterase to tartrate resistant acid phosphatase activity coincides with the in situ expression of osteopontin mRNA. J Histochem Cytochem 43: 1193–201.PubMedGoogle Scholar

Kassem M, Mosekilde L, Rungby J, Mosekilde L, Melsen F, and Eriksen EF (1991) Formation of osteoclasts and osteoblast-like cells in long-term human BM cultures. Apmis 99: 262–8.PubMedGoogle Scholar

Fujikawa Y, Sabokbar A, Neale S, and Athanasou NA (1996) Human osteoclast formation and bone resorption by monocytes and synovial macrophages in rheumatoid arthritis. Annals Rheum Diseases 55: 816–822.CrossRefGoogle Scholar

Pierelli L, Scambia G, Donofrio G, Ciarli M, Fattorossi A, Bonanno G, Menichella G, Battaglia A, Panici PB, Tommasi M, Mancuso S, and Leone G (1997) Generation of multinuclear tartrate-resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34(+) progenitors. Brit J Haematol 96: 64–69.CrossRefGoogle Scholar

Matayoshi A, Brown C, DiPersio JF, Haug J, Abu-Amer Y, Liapis H, Kuestner R, and Pacifici R (1996) Human blood-mobilized hematopoietic precursors differentiate into osteoclasts in the absence of stromal cells. Proc Natl Acad Sci USA 93: 10785–90.CrossRefPubMedGoogle Scholar

Zambonin Zallone A, Grano M, Colucci S, Zigrino P, De Bellis M, Zambonin G, and Serra M (1995) Human osteoclast-like cells from giant cell tumors of bone: a new tool for investigating bone resorption and osteoclast biology. Calcif Tiss lntl 56: S24.Google Scholar

Grano M, Colucci S, De Bellis M, Zigrino P, Argentino L, Zambonin G, Serra M, Scotlandi K, Teti A, and Zambonin Zallone A (1994) New model for bone resorption study in vitro: human osteoclast-like cells from giant cell tumors of bone. J Bone Miner Res 9: 1013–20.PubMedGoogle Scholar

James IE, Dodds RA, Lee-Rykaczewski E, Eichman CF, Connor JR, Hart TK, Maleeff BE, Lackman RD, and Gowen M (1996) Purification and characterization of fully functional human osteoclast precursors. J Bone Miner Res 11: 1608–18.PubMedGoogle Scholar

Ito M, Hsu CT, Naito S, Matsuo T, Onizuka S, Sekine I, Fujii H, and Matsuoka Y (1992) Osteoclast-like giant cell tumour of the gallbladder. Virchows Archiv — A Pathol Anat Histopathol 420: 359–66.CrossRefGoogle Scholar

Mbalaviele G, Orcel P, Morieux C, Nijweide PJ, and de Vernejoul MC (1995) Osteoclast formation from human cord blood MNCs co-cultured with mice embryonic metatarsals in the presence of M-CSF. Bone 16: 171–7.CrossRefPubMedGoogle Scholar

Purton LE, Lee MY, and Torok-Storb B (1996) Normal human peripheral blood MNCs mobilized with granulocyte colony-stimulating factor have increased osteoclastogenic potential compared to nonmobilized blood. Blood 87: 1802–8.PubMedGoogle Scholar

Higuchi S, Tabata N, Tajima M, Ito M, Tsurudome M, Sudo A, Uchida A, and Ito Y (1998) Induction of human osteoclast-like cells by treatment of blood monocytes with anti-fusion regulatory protein-1/CD98 monoclonal antibodies. J Bone Miner Res 13: 44–49.PubMedCrossRefGoogle Scholar

Sarma U and Flanagan AM (1996) Macrophage colony-stimulating factor induces substantial osteoclast generation and bone resorption in human BM cultures. Blood 88: 2531–40.PubMedGoogle Scholar

Oursler MJ, Pederson L, Fitzpatrick L, Riggs BL, and Spelsberg T (1994) Human giant cell tumors of the bone (osteoclastomas) are estrogen target cells. Proc Natl Acad Sci USA 91: 5227–31.PubMedCrossRefGoogle Scholar

Li YP, Chen W, and Stashenko P (1996) Molecular cloning and characterization of a putative novel human osteoclast-specific 116-kDa vacuolar proton pump subunit. Biochem Biophys Res Commun 218: 813–21.PubMedCrossRefGoogle Scholar

Goldring S, Roelke M, Petrison K, Bhan A (1987) Human giant cell tumors of bone, Identification and characterization of cell types. J Clin Investig 79: 483–491.PubMedCrossRefGoogle Scholar

Galvin RJ, Cullison JW, Avioli LV, and Osdoby PA (1994) Influence of osteoclasts and osteoclast-like cells on osteoblast alkaline phosphatase activity and collagen synthesis. J Bone Miner Res 9: 1167–78.PubMedGoogle Scholar

Murrills RJ, Shane E, Lindsay R, and Dempster DW (1989) Bone resorption by isolated human osteoclasts in vitro: effects of calcitonin. J Bone Miner Res 4: 259–68.PubMedGoogle Scholar

Lambrecht JT (1990) Cell cultures of human osteoclasts for testing biomaterials. Deutsche Zahnarztliche Zeitschrift 45: 82–6.PubMedGoogle Scholar

Lambrecht JT and Marks SC, Jr. (1996) Human osteoclast-like cells in primary culture. Clin Anat 9: 41–5.CrossRefPubMedGoogle Scholar

Osdoby P, Tsay A, Rothe L, Collin-Osdoby P, and Goldring S (1997) IL-8 expression is elevated in human osteoclasts associated with arthritic and Paget’s disease as well as in human osteoclast-like cells exposed to inflammatory cytokines. J Bone Miner Res 12: S 199.Google Scholar

Collin-Osdoby P, Anderson F, Sunyer T, Rothe L, Weihe J, Maloney W, Miller G, and Osdoby P (1998) Isolation of bone-resorptive human osteoclasts: Culture, characterization, and hormonal regulation in vitro. (Submitted).Google Scholar

Collin-Osdoby P, Oursler MJ, Webber D, and Osdoby P (1991) Osteoclast-specific monoclonal antibodies coupled to magnetic beads provide a rapid and efficient method of purifying avian osteoclasts. J Bone Miner Res 6: 1353–65.PubMedGoogle Scholar

Collin-Osdoby P, Li L, Rothe L, Anderson F, Kirsch D, Oursler MJ, and Osdoby P (1998) Inhibition of avian osteoclast bone resorption by monoclonal antibody 121F: A mechanism involving the osteoclast free radical system. J Bone Miner Res 13: 67–78.PubMedCrossRefGoogle Scholar

Toyasawa S, Ogawa Y11, Chang C, Hong S, Yagi T, Kuwahara H, Wakasa K, and Sakurai M (1991) Histochemistry of tartrate-resistant acid phosphatase and carbonic anhydrase isoenzyme II in osteoclast-like giant cells in bone tumors. Virchows Archiv A Pathol Anat 418: 255–261.CrossRefGoogle Scholar

Drake F, Dodds R, James I, Connor J, Debouck C, Richardson S, Lee-Rykaczewski E, Coleman L, Rieman D, Barthlow R, Hastings G, and Gowen M (1996) Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts. J Biol Chem 271: 12511–12516.PubMedCrossRefGoogle Scholar

Kasten T, Collin-Osdoby P, Patel N, Osdoby P, Krukowski M, Misko T, Settle S, Currie M, and Nickols A (1994) Potentiation of osteoclast bone resorptive activity by inhibition of nitric oxide synthase. Proc Natl Acad Sci USA 91: 3569–3573.PubMedCrossRefGoogle Scholar

Murrills RJ, Stein LS, Horbert WR, and Dempster DW (1992) Effects of phorbol myristate acetate on rat and chick osteoclasts. JBone Miner Res 7: 415–23.Google Scholar

Pacifici R (1996) Estrogen, cytokines, and pathogenesis of postmenopausal osteoporosis. J Bone Miner Res 11: 1043–1051.PubMedGoogle Scholar

Gay C (1991) Avian osteoclasts. Calcif Tiss Intl 49: 153–154.CrossRefGoogle Scholar