Abstract
Serum-free media are more and more used for different cell lines. We report here the preliminary results obtained with serum-free prototype media (SFI and SF2, property of SB Bio) in order to cultivate MRC-5 cells without serum: i) various serum replacement components were mixed to medium and tested on MRC-5 growth which was evaluated by the MTT method; ii) the impact on MRC-5 growth was confirmed by DNA measurements; iii) the prototype media were also tested on the cell growth of MRC-5 in T-flasks (175 cm2).
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© 1999 Kluwer Academic Publishers
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Maggetto, C. et al. (1999). Development of a Serum-Free Medium for MRC-5 Culture. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_98
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DOI: https://doi.org/10.1007/0-306-46875-1_98
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
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