Multiplicity and Time of Infection as Tools to Maximize Virus-Like Particle Production by Insect Cells
Commercial use of vaccines based on virus-like particles (VLPs) demand effective production strategies. However, the production of VLPs is very complex, as several recombinant proteins have to be simultaneously produced, and efficiently assembled. In this work, rational strategies to obtain recombinant double shelled rotavirus-like particles (dRLPs), using the insect cell-baculovirus expression system, are presented.
KeywordsInsect Cell Simultaneous Expression Insect Cell Culture Recombinant Baculoviruses Polyhedrin Promoter
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