Telomere Shortening in Cancer Cells by Electrolyzed-Reduced Water



Electroly-reduced water (ERW) which is produced near cathode during electrolysis of water scavenges reactive oxygen species and protects DNA from oxidative damage (Shirahata et al., 1997). Most of cancer cells exhibit high telomerase activity to elongate telomere length, insuring their immortality. Here we found that cultivation of human lung adenocarciomaA549, human uterine cancer HeLaand human normal fibroblast TIG-1 cells were growth inhibited in medium containing ERW and drastic morphological changes occurred in A549 and HeLa cancer cells but not in TIG-1 cells. Telomerase activity did not change but telomere length became shorter depending upon cell division in medium containing ERW. Telomere binding activities of telomere binding proteins in cancer cells decreased in medium containing ERW, suggesting that ERW inhibit the binding of’ telomerase to telomere region via telomere binding proteins, resulting in the shortening of telomerelength.


A549 Cell Telomere Length Active Oxygen Species Telomeric Repeat Amplification Protocol Lung Adenocarcinoma A549 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Burdon, R. H., Alliangana, D., and Gill, V. (1994) Endogeneously generated active oxygen species and cellular glutathione levels in relation to BHK-21 cell proliferation. Free Rad. Res., 21, 121–133.Google Scholar
  2. 2.
    Burdon, R. H. and Gill, V. (1993) Cellulary generated active oxygen species and Hela cell proliferation. Free Rad. Res. Comms., 19, 203–213.CrossRefGoogle Scholar
  3. 3.
    Katakura Y., Yamamolo, Y., Miyake, O., Yasuda, T., Uehara, N., Nakata, E., Kawamoto, S., and Shirahata, S. (1997) Bidrectionalregulation of telomerase activity in a sublinederived from human lung adenocarcinoma. Biochem. Biophys. Res. Comms. 237, 313–317.Google Scholar
  4. 4.
    Shirahata, S., Kabayama, S., Kusumoto, K., Gotoh, M., Teruya, K., Otsubo, K., Morisawa, S., Hayashi, H., and Katakura, K. (1998) Electrolyzed reduced water which can scavenge active oxygen species suppresses cell growth and regulates gene expression of animal cells. In: O.-W. Merten et al. (eds.), New Development and New Applications in Animal Cell Technology, pp.93–96, Kluwer Academic Publishers, the Netherlands.Google Scholar
  5. 5.
    Shirahata, S., Kabayama, S., Miura, T., Kusumoto, K., Gotoh, M., Hayashi, H., Otusbo, K., Morisawa, S., and Katakura, Y (1997) Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochem. Biophys. Res. Commns., 234, 269–274.Google Scholar
  6. 6.
    Toyokuni, S., Okamoto, K., Yodoi, J., and Hiai, H. (1995) Persistent oxidative stress in cancer. FEBS Letters, 358, 1–3.CrossRefPubMedGoogle Scholar
  7. 7.
    von Zglinicki, T., Saretzki, G., Docke, W., and Lotze, C. (1995) Mild hyperoxia shortens telomeres andinhibits proliferation of fibroblasts: amodel for senescence? Exp. Cell Res. 220, 185–193.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  1. 1.Graduate School of Genetic Resources TechnologyKyushu UniiversityFukuokaJapan
  2. 2.Nihon Trim Co. Ltd.OsakaJapan
  3. 3.Water Instutitute Nisshin Building 9FTokyoJapan

Personalised recommendations