The Expression of Polysaccharide Capsules in Escherichia coli

A Molecular Genetic Perspective
  • Ian S. Roberts

Keywords

Cytoplasmic Membrane Capsular Polysaccharide COLI Group Polysialic Acid Polysaccharide Capsule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Referemces

  1. Bailey, M. J., Hughes, C., and Koronakis, V., 1997, RfaH and the opselement, components of a novel system controlling bacterial transcription elongation, Mol. Microbiol. 26:845–851.CrossRefPubMedGoogle Scholar
  2. Bayer, M. E., 1991, Zones of membrane adhesion in cryofixed envelope of Escherichia coli, J Struct. Biol. 107:268–280.CrossRefPubMedGoogle Scholar
  3. Bayer, M. E. and Thurow, H., 1977, Polysaccharide capsule of Escherichia coli: Microscope study of its size, structure, and sites of synthesis, J. Bacteriol. 130:911–936.PubMedGoogle Scholar
  4. Bayer, M. E., Baye, M. H., Lunn, C. A., and Pigiet, V., 1987, Association of thioredoxin with the inner membrane and adhesion sites in Escherichia coli, J. Bacteriol. 169:2659–2666.PubMedGoogle Scholar
  5. Beacham, I. R., 1979, Periplasmic enzymes in Gram-negative bacteria. Int. J. Biochem. 10:877–883.CrossRefPubMedGoogle Scholar
  6. Bilge, S. S., Apostol, J. M., Fullner, K. J., and Moseley, S. L., 1993, Transcription organi-sation of the F1854 fimbrial adhesin determinant of Escherichia coli, Mol. Microbiol. 7:993–1006.PubMedGoogle Scholar
  7. Blattner, F. R., Plunkett III, G., Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirk-patrick, N. W., Goeden, M. A., Rose, J. D., Mau, B., and Shao, Y., 1997, The complete genome sequence of Escherichia coli K-12, Science 277:1453–1462.CrossRefPubMedGoogle Scholar
  8. Bliss, J. M., and Silver, R. P., 1996, Coating the surface: A model for expression of capsular polysialic acid in Escherichia coli K1, Mol. Microbiol. 21:221–231.CrossRefPubMedGoogle Scholar
  9. Boulnois, G. J., Roberts, I. S., Hodge, R., Hardy, K., Jann, K., and Timmis, K. N., 1987, Analysis of the K1 capsule biosynthesis genes of Escherichia coli: Definition of three functional regions for capsule production, Mol. Gen. Genet. 208:242–246.CrossRefPubMedGoogle Scholar
  10. Boulnois, G. J., Drake, R., Pearce, R., and Roberts, I. S., 1992, Genome diversity at the serA-linked capsule locus in Escherichia coli, FEMS Microbiol. Lett. 100:121–124.CrossRefGoogle Scholar
  11. Bronner, D., Sieberth, V., Pazzani, C., Roberts, I. S., Boulnois, G. J., Jann, B., and Jann, K., 1993, Expression of the capsular K5 polysaccharide of Escherichia coli: Biochemical and electron microscopic analyses of mutants with defects in region 1 of the K5 gene cluster, J. Bacteriol. 175:5984–5992.PubMedGoogle Scholar
  12. Cheetham, B. F., and Katz, M. E., 1995, A role for bacteriophages in the evolution and transfer of bacterial virulence determinants, Mol. Microbiol. 18:201–208.CrossRefPubMedGoogle Scholar
  13. Cieslewicz, M., and Vimr, E., 1996, Thermoregulation of kpsF, the first region 1 gene in the kps locus for polysialic acid biosynthesis in Escherichia coli Kl, J. Bacteriol. 178:3212–3220.PubMedGoogle Scholar
  14. Cieslewicz, M., and Vimr, E., 1997, Reduced polysialic acid capsule expression in Escherichia coli Kl mutants with chromosomal defects in kpsF, Mol. Microbiol. 26:237–249.CrossRefPubMedGoogle Scholar
  15. Clarke, B. R., Pearce, R., and Roberts, I. S., 1999, Genetic organisation of the Escherichia coli K10 capsule gene cluster: identification and characterisation of two conserved regions in group III capsule gene clusters encoding polysaccharide transport functions, J. Bacteriol. 181:2279–2285.PubMedGoogle Scholar
  16. Costerton, J. W., Chjeng, K.-J., Geesey, G. G., Ladd, T. I., Nickel, J. C., Dasgupta, M., and Marrie, T., 1987, Annu. Rev. Microbiol. 41:435–466.CrossRefPubMedGoogle Scholar
  17. Dinh, T, Paulsen, I. T, and Saier, M. H., 1994, A family of extracytoplasmic proteins that allow transport of large molecules across the outer membrane of gram-negative bacteria, J. Bacteriol. 176:3825–3831.PubMedGoogle Scholar
  18. Dorman, C. J., and Porter, M. E., 1998, The Shigella virulence gene regulatory cascade: A paradigm of bacterial gene control mechanisms, Mol. Microbiol. 29:677–684.CrossRefPubMedGoogle Scholar
  19. Drummelsmith, J., Amor, P. A., and Whitfield, C., 1997, Polymorphism, duplication and IS-1-mediated rearrangement in the his-rfb-gnd reion of Escherichia coli strains with group IA capsular-K-antigens, J. Bacteriol. 179:3232–3238.PubMedGoogle Scholar
  20. Finke, A., Bronner, D., Nikolaev, A. V., Jann, B., and Jann, K., 1991, Biosynthesis of the Escherichia coli K5 polysaccharide, a representative of group II capsular polysaccharides: Polymerization in vitro and characterization of the product, J. Bacteriol. 173:4088–4094.PubMedGoogle Scholar
  21. Freundlich, M., Ramani, N., Mathew, E., Sirko, A., and Tsui, P., 1992, The role of integration host factor in gene expression in Escherichia coli, Mol. Microbiol. 6:2557–2563.PubMedGoogle Scholar
  22. Gotschlich, E. C., Fraser, B. A., Nishimura, O., Robbins, J. B., and Liu, T-Y., 1981, Lipid on capsular polysaccharides ogf gram-negative bacteria, J. Biol. Chem. 256:8915–8921.PubMedGoogle Scholar
  23. Griffiths, G., Cook, N. J., Gottfridson, E., Lind, T., Lidholt, K., and Roberts, I. S., 1998, Characterization of the glycosyltransferase enzyme from the Escherichia coli K5 capsule gene cluster and identification and chracterization of the glucuronyl active site, J. Biol. Chem. 273:11752–11757.CrossRefPubMedGoogle Scholar
  24. Guihard, G., Boulanger, P., Bénédetti, H., Loubés, R., Bernard, M., and Letellier, L., 1994, Colicin A and Tol proteins involved in its translocation are preferentially located in the contact sites between the inner and outer membranes of Escherichia coli cells, J. Biol. Chem. 269:5874–5880.PubMedGoogle Scholar
  25. Hobbs, M., and Reeves, P. R., 1994, The JUMPstart sequence: A 39 bp element common to several polysaccharide gene clusters, Mol. Microbiol. 12:855–856.PubMedGoogle Scholar
  26. Jann, B., and Jann, K., 1990, Structure and biosynthesis of the capsular antigens of Escherichia coli, Curr. Top. Microbiol. Immunol. 150:19–42.PubMedGoogle Scholar
  27. Jann, K., and Jann, B., 1992, Capsules of Escherichia coli, expression and biological significance, Can. J. Microbiol. 38:705–710.PubMedCrossRefGoogle Scholar
  28. Jayaratne, P., Keenleyside, W. J., MacLachlan, P. R., Dodgson, C., and Whitfield, C., 1993, Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides, J. Bacteriol. 175:5384–5394.PubMedGoogle Scholar
  29. Keenleyside, W. J., Jayaratne, P., MacLachlan, P. R., and Whitfield, C., 1992, The rcsA gene of Escherichia coli O9:K30:H12 is involved in the expression of the serotype-specific group I K (capsular) antigen, J. Bacteriol. 174:8–16.PubMedGoogle Scholar
  30. Klug, G., 1993, The role of mRNA degradation in regulated expression of bacterial photosynthesis genes, Mol. Microbiol. 9:1–7.PubMedGoogle Scholar
  31. Letoff, S., Delepelaire, P., and Wandersman, C., 1996, Protein secretion in gram-negative bacteria: Assembly of the three components of the ABC protein mediated exporters is ordered and promoted by substrate binding, EMBO J 15:5804–58011.Google Scholar
  32. Marolda, C. L., and Valvano, M. A., 1998, Promoter region of the Escherichia coli O7-specific lipopolysaccharide gene cluster: Structural and functional characterization of an upstream untranslated mRNA sequence, J. Bacteriol. 180:3070–3079.PubMedGoogle Scholar
  33. Moxon, E. R., and Kroll, J. S., 1990, The role of bacterial polysaccharide capsules as virulence factors, Curr. Top. Microbiol. Immunol. 150:65–86.PubMedGoogle Scholar
  34. Ørskov, I., and Nyman, K., 1974, Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli, J. Bacteriol. 120:43–51.PubMedGoogle Scholar
  35. Ørskov, F., and Ørskov, I., 1992, Escherichia coli serotyping and disease in man and animals, Can. J. Microbiol. 38:699–704.PubMedGoogle Scholar
  36. Paulsen, I. T., Beness, A. M., and Saier, M. H. J., 1997, Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria, Microbiology 143:2685–2699.PubMedGoogle Scholar
  37. Pavelka, M. S., Hayes, S. F., and Silver, R. P., 1994, Characterisation of KpsT, the ATP-binding component of the ABC-transporter involved in the export of capsular polysialic acid in Escherichia coli K1, J. Biol. Chem. 269:20149–20158.PubMedGoogle Scholar
  38. Pazzani, C., Rosenow, C., Boulnois, G. J., Bronner, D., Jann, K., and Roberts, I. S., 1993, Molecular analysis of region 1 of the Escherichia coli K5 antigen gene cluster: A region encoding proteins involved in cell surface expression of capsular polysaccharide, J. Bacteriol. 175:5978–5983.PubMedGoogle Scholar
  39. Pearce, R., and Roberts, I. S., 1995, Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters, J. Bacteriol. 177:3992–3997.PubMedGoogle Scholar
  40. Perna, N. T., Mayhew, G. F, Pósfal, G., Elliott, S., Donnenberg, M. S., Kaper, J. B., and Blattner, F. R., 1998, Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7, Infect. Immun. 66:3810–3817.PubMedGoogle Scholar
  41. Petit, C., Rigg, G. P., Pazzani, C., Smith, A., Sieberth, V., Stevens, M., Boulnois, G., Jann, K., and Roberts, I. S., 1995, Region 2 of the Escherichia coli K5 capsule gene cluster encoding proteins for the biosynthesis of the K5 polysaccharide, Mol. Microbiol. 17:611–620.CrossRefPubMedGoogle Scholar
  42. Pigeon, R. P., and Silver, R. P., 1994, Topological and mutational analysis of KpsM, the hydrophobic component of the ABC-transporter involved in the export of polysialic acid in Escherichia coli Kl, Mol. Microbiol. 14:871–881.PubMedGoogle Scholar
  43. Richardson, J. P., 1991, Preventing the synthesis of unused transcripts by Rho factor, Cell 64:1047–1049.CrossRefPubMedGoogle Scholar
  44. Rick, P., and Silver, R. P., 1996, Enterobacterial common antigen and capsular polysaccharides, in: Escherichia coli and Salmonella: Cellular and Molecular Biology (F. C. Neidhardt, ed.), ASM Press Washington, DC, pp. 104–122.Google Scholar
  45. Rigg, G. P., Barrett, B., and Roberts, I. S., 1998, The localization of KpsC, S and T and KfiA, C and D proteins involved in the biosynthesis of the Escherichia coli K5 capsular polysaccharide: Evidence for a membrane-bound complex, Microbiology 144:2904–2914.CrossRefGoogle Scholar
  46. Roberts, I. S., 1995, Bacterial polysaccharides in sickness and in health, Microbiology 141:2023–2031.PubMedGoogle Scholar
  47. Roberts, I. S., 1996, The biochemistry and genetics of capsular polysaccharide production in bacteria, Annu. Rev. Microbiol. 50:285–315.CrossRefPubMedGoogle Scholar
  48. Roberts, I. S., Mountford, R., High, N., Bitter-Suerman, D., Jann, K., Timmis, K. N., and Boulnois, G. J., 1986, Molecular cloning and analysis of the genes for production of the K5, K7, K12, and K92 capsular polysaccharides of Escherichia coli, J. Bacteriol. 168:1228–1233.PubMedGoogle Scholar
  49. Roberts, I. S., Mountford, R., Hodge, R., Jann, K., and Boulnois, G. J., 1988, Common organization of gene clusters for the production of different capsular polysaccharides (Kantigens) in Escherichia coli, J. Bacteriol. 170:1305–1310.PubMedGoogle Scholar
  50. Rosenow, C., Esumeh, P., Roberts, I. S., and Jann, K., 1995, Characterisation and localizsation of the KpsE protein of Escherichia coli K5, which is involved in polysaccharide export, J. Bacteriol. 177:1137–1143.PubMedGoogle Scholar
  51. Russo, T. A., Wenderoth, S., Carlino, U. B., Merrick, J. M., and, A. J.,, L., 1998, Isolation, genomic organization, and analysis of the group III capsular polysaccharide genes kpsD, kpsM, kpsT, and kpsE from an extraintestinal isolate of Escherichia coli (CP9, O4/K54/H5), J. Bacteriol. 180:338–349.PubMedGoogle Scholar
  52. Sieberth, V., Jann, B., and Jann, K., 1993, Structure of the K10 capsular antigen from Escherichia coli O11:K10:H10, a polysaccharide containing 4,6-dideoxy-4-malonylamino-d-glucose, Carbohydr. Res. 246:219–228.CrossRefPubMedGoogle Scholar
  53. Silver, R. P., Vann, W. F., and Aaronson, W., 1984, Genetic and molecular analyses of Escherichia coli Kl antigen genes, J. Bacteriol. 157:568–575.PubMedGoogle Scholar
  54. Simpson, D. A., Hammarton, T. C., and Roberts, I. S., 1996, Transcriptional organization and regulation of expression of region 1 of the Escherichia coli K5 capsule gene cluster, J. Bacteriol. 178:6466–6474.PubMedGoogle Scholar
  55. Smith, A. N., Boulnois, G. J., and Roberts, I. S., 1990, Molecular analysis of the Escherichia coli K5 kps locus: Identification and characterisation of an inner-membrane capsular polysaccharide transport system, Mol. Microbiol. 4:1863–1869.PubMedCrossRefGoogle Scholar
  56. Steenbergen, S. M., and Vimr, E. R., 1990, Mechanism of polysialic acid chain elongation in Escherichia coli Kl, Mol. Microbiol. 4:603–611.PubMedGoogle Scholar
  57. Stevens, M. P., Clarke, B. R., and Roberts, I. S., 1997, Regulation of the Escherichia coli K5 capsule gene cluster by transcription antitermination, Mol. Microbiol. 24:1001–1012.CrossRefPubMedGoogle Scholar
  58. Timmerman, K. P., and Tu, C. P. D., 1985, Complete sequence of IS3, Nucleic Acids Res. 13:2127–2139.PubMedGoogle Scholar
  59. Troy, F. A., 1995, Sialobiology and the polysialic acid glycotype: occurrence, structure, function, synthesis and glycopathology, in: Biology of the Sialic Acids (A. Rosenberg, ed.), Plenum Press, New York, pp. 95–144.Google Scholar
  60. Wacharotayankun, R., Arakawa, Y., Ohta, M., Tanaka, K., Akashi, T., Mori, M., and Kato, N., 1993, Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ, Infect. Immun. 61:3164–3174.PubMedGoogle Scholar
  61. Whitfield, C., and Roberts, I. S., 1999, Structure, assembly and regulation of expression of capsules in Escherichia coli, Mol. Microbiol. 31:1307–1320.CrossRefPubMedGoogle Scholar
  62. Whitfield, C., and Valvano, M. A., 1993, Biosynthesis and expression of cell surface polysaccharides in gram-negative bacteria, Adv. Microb. Physiol. 35:135–246.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Ian S. Roberts
    • 1
  1. 1.School of Biological SciencesUniversity of ManchesterManchesterEngland

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