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Molecular Identification of Traditional Medicinal Materials

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Molecular Pharmacognosy

Abstract

Traditional medicines are consumed by 80% of the population in the world for health maintenance and disease treatment. The adulteration and substitution of source materials are life-threatening problems that have grown along with its popularity. Consequently, a reliable identification method is important for the safety and quality assurance of traditional Chinese medicine (TCM) materials. Molecular techniques provide an alternative means to conventional organoleptic and chemical identification methods and are superior in terms of their accuracy, sensitivity, resolution and reproducibility. Since the early 1990s, a number of molecular techniques have been developed to identify traditional medicinal materials based on DNA fingerprinting, specific amplification, DNA sequencing, DNA microarrays and fluorescence detection techniques. Molecular techniques are capable of differentiating traditional medicinal materials from their adulterants and substitutes in closely related species, subspecies, variants, cultivars and species from different localities, and, in some cases, they can distinguish the growth year and herb quality. This chapter introduces the major molecular identification techniques and reviews their applications in the identification of animal and botanical medicinal materials.

Ming Li and Chao Jiang are equally contributed for this chapter.

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Authors and Affiliations

  1. Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China

    Ming Li, Paul Pui-Hay & Pang-Chui Shaw

  2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China

    Chao Jiang & Yuan Yuan

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  1. Ming Li
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  2. Chao Jiang
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Correspondence to Pang-Chui Shaw or Yuan Yuan .

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  1. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China

    Lu-qi Huang

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Li, M., Jiang, C., Pui-Hay, P., Shaw, PC., Yuan, Y. (2019). Molecular Identification of Traditional Medicinal Materials. In: Huang, Lq. (eds) Molecular Pharmacognosy. Springer, Singapore. https://doi.org/10.1007/978-981-32-9034-1_2

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