Abstract
Thyroid hormones are key hormones involved in growth and development. Changes in their levels can cause embryonic brain developmental damage in the first trimester. Studies have shown that polybrominated diphenyl ethers (PBDEs) have developmental neurotoxicity as environmental pollutants, and exposure during pregnancy can cause irreversible brain damage in offspring, similar to the interference effects of thyroid hormones, but its mechanism has not yet been understood. Since the physiological environment for placental cells is highly hypoxic, in the current study, the human placenta-derived JEG cells were cultured at 1% oxygen, 4% carbon dioxide and 94% nitrogen, to reflect in vivo scenario, and the possible protection of taurine on BDE 209-mediated toxicity in JEG cells was studied. Our data showed that different concentrations of BDE 209 can have profound effects on cell viability and placental deiodinase 3 expression under hypoxic culture condition. Taurine was found to improve BDE 209-induced reductions in cell viability and altered gene and protein expressions of placental deiodinases. The results provide a reference for the establishment of early biomarkers and effective preventive measures.
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- Dio:
-
Deiodinase
- JEG:
-
Human trophoblastic JEG-3 cells
- MTT:
-
4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide
- PBDEs:
-
Polybrominated diphenyl ethers
- TH:
-
Thyroid hormone
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Acknowledgements
This research was supported by the National Natural Science Foundation of China (81773389), Dalian Tech Star Programme (2016RQ044) and China’s Post-doctoral Science Fund (2016M591438).
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Cheng, Y. et al. (2019). Alteration of Placental Deiodinase 3 Expression by BDE 209 and Possible Protection by Taurine in Human Placenta-Derived JEG Cells Under Hypoxia. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_67
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DOI: https://doi.org/10.1007/978-981-13-8023-5_67
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