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Taurine Chloramine Inhibits Osteoclastic Differentiation and Osteoclast Marker Expression in RAW 264.7 Cells

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Book cover Taurine 11

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1155))

Abstract

Taurine is an abundant sulfur-containing amino acid in myeloid cells. It undergoes halogenation in activated phagocytes and is converted to taurine chloramine (TauCl) and taurine bromamine. Bone homeostasis is mediated by the balance between bone-forming osteoblasts and bone-resorbing osteoclasts. Osteoclasts are bone-resorbing multinucleated cells differentiated from monocyte/macrophage precursor cells in response to receptor activator of NF-κB ligand (RANKL). In this study, we investigated the effect of TauCl on RANKL-induced osteoclastogenesis from RAW 264.7 macrophages. TauCl inhibited the formation of multi-nucleated osteoclast and the activity of tartrate-resistant acid phosphatase (TRAP). TauCl decreased the mRNA expression of osteoclast markers, such as TRAP, cathepsin K, and calcitonin receptor. TauCl also inhibited expression of the transcription factors, c-Fos and nuclear factor of activated T cells, which are important for osteoclast differentiation. These results suggest that TauCl might be used as a therapeutic agent to treat bone diseases associated with excessive bone resorption.

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Abbreviations

BMMs:

Bone marrow-derived monocyte/macrophage precursor cells

CTR:

Calcitonin receptor

CTSK:

Cathepsin K

M-CSF:

Macrophage colony-stimulating factor

NFATc1:

Nuclear factor of activated T cell 1

NF-κB:

Nuclear factor-κB

RANKL:

Receptor activator of nuclear factor-κB ligand

TauCl:

Taurine chloramine

TNF:

Tumor necrosis factor

TRAF6:

TNF receptor-associated factor 6

TRAP:

Tartrate-resistant acid phosphatase

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Acknowledgements

This work was supported by the Inha University Research Fund.

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Authors and Affiliations

  1. Laboratory of Leukocyte Signaling Research, Department of Pharmacology, Inha University School of Medicine, Incheon, South Korea

    In Soon Kang & Chaekyun Kim

Authors
  1. In Soon Kang
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  2. Chaekyun Kim
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Correspondence to Chaekyun Kim .

Editor information

Editors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China

    Jianmin Hu

  2. Department of Occupational and Environmental Health, Dalian Medicine University, Dalian, Liaoning, China

    Fengyuan Piao

  3. Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, USA

    Stephen W. Schaffer

  4. Department of Biology, Center for Developmental Neuroscience, College of Staten Island, Staten Island, NY, USA

    Abdeslem El Idrissi

  5. Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA

    Jang-Yen Wu

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Kang, I.S., Kim, C. (2019). Taurine Chloramine Inhibits Osteoclastic Differentiation and Osteoclast Marker Expression in RAW 264.7 Cells. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_6

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