Abstract
Heterogeneous phenotypes of cancer cells enable them to adapt to various environments. The heterogeneity results from diversity of genome, transcriptome, and epigenome at a single-cell level. The C1 system can automatically perform single-cell capture and whole genome amplification (WGA) or whole transcription amplification (WTA) by MDA or Smart-Seq, respectively. Here, we describe the protocols for WGA and WTA from a single cell by using the C1 system and the protocols for sequence library preparation from amplified gDNA and cDNA. We also described about the computational analysis for single-cell data of cancer.
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Acknowledgments
We would like to express our gratitude to Y Kuze, T Horiuchi, K Kunigo, Y Ishikawa, and K Imamura for helpful advice in writing this manuscript. This work was supported by MEXT KAKENHI Grant Number 221S0002.
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Seki, M., Suzuki, A., Sereewattanawoot, S., Suzuki, Y. (2019). Single-Cell DNA-Seq and RNA-Seq in Cancer Using the C1 System. In: Suzuki, Y. (eds) Single Molecule and Single Cell Sequencing. Advances in Experimental Medicine and Biology, vol 1129. Springer, Singapore. https://doi.org/10.1007/978-981-13-6037-4_3
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DOI: https://doi.org/10.1007/978-981-13-6037-4_3
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