Skip to main content
SpringerLink
Log in

Intestinal Barrier for Nematodes Against Toxicity of Environmental Toxicants or Stresses

  • Chapter
  • First Online:
Target Organ Toxicology in Caenorhabditis elegans

  • 342 Accesses

Abstract

In nematodes, the intestinal barrier is one of the primary biological barriers for animals against the toxicity from environmental toxicants or stresses. We here first introduced the evidence to highlight the crucial role of intestinal barrier against the toxicity of environmental toxicants. Moreover, we introduced and discussed the underlying molecular basis for intestinal barrier against the toxicity of environmental toxicants or stresses. The modulation of intestinal integrity during the aging and the association between intestinal barrier and stress-related signaling pathways were also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wang D-Y (2018) Nanotoxicology in Caenorhabditis elegans. Springer, Singapore

    Book  Google Scholar 

  2. Wang D-Y (2018) Molecular toxicology in Caenorhabditis elegans. Springer, Singapore

    Book  Google Scholar 

  3. Xiao G-S, Zhao L, Huang Q, Yang J-N, Du H-H, Guo D-Q, Xia M-X, Li G-M, Chen Z-X, Wang D-Y (2018) Toxicity evaluation of Wanzhou watershed of Yangtze Three Gorges Reservoir in the flood season in Caenorhabditis elegans. Sci Rep 8:6734

    Article  Google Scholar 

  4. Yin J-C, Liu R, Jian Z-H, Yang D, Pu Y-P, Yin L-H, Wang D-Y (2018) Di (2-ethylhexyl) phthalate-induced reproductive toxicity involved in DNA damage-dependent oocyte apoptosis and oxidative stress in Caenorhabditis elegans. Ecotoxicol Environ Saf 163:298–306

    Article  CAS  Google Scholar 

  5. Xiao G-S, Zhao L, Huang Q, Du H-H, Guo D-Q, Xia M-X, Li G-M, Chen Z-X, Wang D-Y (2018) Biosafety assessment of water samples from Wanzhou watershed of Yangtze Three Gorges Reservoir in the quiet season in Caenorhabditis elegans. Sci Rep 8:14102

    Article  Google Scholar 

  6. Wang D-Y, Yu Y-L, Li Y-X, Wang Y, Wang D-Y (2014) Dopamine receptors antagonistically regulate behavioral choice between conflicting alternatives in C. elegans. PLoS One 9:e115985

    Article  Google Scholar 

  7. Li Y-X, Wang Y, Hu Y-O, Zhong J-X, Wang D-Y (2011) Modulation of the assay system for the sensory integration of 2 sensory stimuli that inhibit each other in nematode Caenorhabditis elegans. Neurosci Bull 27:69–82

    Article  Google Scholar 

  8. Yu Y-L, Zhi L-T, Wu Q-L, Jing L-N, Wang D-Y (2018) NPR-9 regulates innate immune response in Caenorhabditis elegans by antagonizing activity of AIB interneurons. Cell Mol Immunol 15:27–37

    Article  CAS  Google Scholar 

  9. Zhi L-T, Yu Y-L, Jiang Z-X, Wang D-Y (2017) mir-355 functions as an important link between p38 MAPK signaling and insulin signaling in the regulation of innate immunity. Sci Rep 7:14560

    Article  Google Scholar 

  10. Yu Y-L, Zhi L-T, Guan X-M, Wang D-Y, Wang D-Y (2016) FLP-4 neuropeptide and its receptor in a neuronal circuit regulate preference choice through functions of ASH-2 trithorax complex in Caenorhabditis elegans. Sci Rep 6:21485

    Article  CAS  Google Scholar 

  11. Sun L-M, Zhi L-T, Shakoor S, Liao K, Wang D-Y (2016) microRNAs involved in the control of innate immunity in Candida infected Caenorhabditis elegans. Sci Rep 6:36036

    Article  CAS  Google Scholar 

  12. Ruan Q-L, Qiao Y, Zhao Y-L, Xu Y, Wang M, Duan J-A, Wang D-Y (2016) Beneficial effects of Glycyrrhizae radix extract in preventing oxidative damage and extending the lifespan of Caenorhabditis elegans. J Ethnopharmacol 177:101–110

    Article  CAS  Google Scholar 

  13. McGhee JD (2007) The C. elegans intestine. WormBook. https://doi.org/10.1895/wormbook.1.133.1

  14. Zhao Y-L, Wu Q-L, Li Y-P, Wang D-Y (2013) Translocation, transfer, and in vivo safety evaluation of engineered nanomaterials in the non-mammalian alternative toxicity assay model of nematode Caenorhabditis elegans. RSC Adv 3:5741–5757

    Article  CAS  Google Scholar 

  15. Yu X-M, Guan X-M, Wu Q-L, Zhao Y-L, Wang D-Y (2015) Vitamin E ameliorates the neurodegeneration related phenotypes caused by neurotoxicity of Al2O3-nanoparticles in C. elegans. Toxicol Res 4:1269–1281

    Article  CAS  Google Scholar 

  16. Wang Q-Q, Zhao S-Q, Zhao Y-L, Rui Q, Wang D-Y (2014) Toxicity and translocation of graphene oxide in Arabidopsis plants under stress conditions. RSC Adv 4:60891–60901

    Article  CAS  Google Scholar 

  17. Zhao L, Kong J-T, Krasteva N, Wang D-Y (2018) Deficit in epidermal barrier induces toxicity and translocation of PEG modified graphene oxide in nematodes. Toxicol Res 7:1061–1070

    Article  CAS  Google Scholar 

  18. Wu Q-L, Li Y-X, Li Y-P, Zhao Y-L, Ge L, Wang H-F, Wang D-Y (2013) Crucial role of biological barrier at the primary targeted organs in controlling translocation and toxicity of multi-walled carbon nanotubes in nematode Caenorhabditis elegans. Nanoscale 5:11166–11178

    Article  CAS  Google Scholar 

  19. Ding X-C, Rui Q, Wang D-Y (2018) Functional disruption in epidermal barrier enhances toxicity and accumulation of graphene oxide. Ecotoxicol Environ Saf 163:456–464

    Article  CAS  Google Scholar 

  20. Zhi L-T, Qu M, Ren M-X, Zhao L, Li Y-H, Wang D-Y (2017) Graphene oxide induces canonical Wnt/β-catenin signaling-dependent toxicity in Caenorhabditis elegans. Carbon 113:122–131

    Article  CAS  Google Scholar 

  21. Ren M-X, Zhao L, Lv X, Wang D-Y (2017) Antimicrobial proteins in the response to graphene oxide in Caenorhabditis elegans. Nanotoxicology 11:578–590

    Article  CAS  Google Scholar 

  22. Qu M, Li Y-H, Wu Q-L, Xia Y-K, Wang D-Y (2017) Neuronal ERK signaling in response to graphene oxide in nematode Caenorhabditis elegans. Nanotoxicology 11:520–533

    Article  CAS  Google Scholar 

  23. Zhao Y-L, Wu Q-L, Wang D-Y (2016) An epigenetic signal encoded protection mechanism is activated by graphene oxide to inhibit its induced reproductive toxicity in Caenorhabditis elegans. Biomaterials 79:15–24

    Article  CAS  Google Scholar 

  24. Zhao Y-L, Wu Q-L, Wang D-Y (2015) A microRNAs-mRNAs network involved in the control of graphene oxide toxicity in Caenorhabditis elegans. RSC Adv 5:92394–92405

    Article  CAS  Google Scholar 

  25. Wu Q-L, Zhao Y-L, Fang J-P, Wang D-Y (2014) Immune response is required for the control of in vivo translocation and chronic toxicity of graphene oxide. Nanoscale 6:5894–5906

    Article  CAS  Google Scholar 

  26. Miyadera H, Amino H, Hiraishi A, Taka H, Murayama K, Miyoshi H, Sakamoto K, Ishii N, Hekimi S, Kita K (2001) Altered quinone biosynthesis in the long-lived clk-1 mutants of Caenorhabditis elegans. J Biol Chem 276:7713–7716

    Article  CAS  Google Scholar 

  27. Carberry K, Wiesenfahrt T, Geisler F, Stocker S, Gerhardus H, Uberbach D, Davis W, Jorgensen E, Leube RE, Bossinger O (2012) The novel intestinal filament DLG-1. Development 139:1851–1862

    Article  CAS  Google Scholar 

  28. Gobel V, Barrett PL, Hall DH, Fleming JT (2004) Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1. Dev Cell 6:865–873

    Article  Google Scholar 

  29. Croce A, Cassata G, Disanza A, Gagliani MC, Tacchetti C, Malabarba MG, Carlier MF, Scita G, Baumeister R, Di Fiore PP (2004) A novel actin barbed-endcapping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegans. Nat Cell Biol 6:1173–1179

    Article  CAS  Google Scholar 

  30. Nance J, Priess JR (2002) Cell polarity and gastrulation in C. elegans. Development 129:387–397

    CAS  PubMed  Google Scholar 

  31. Wu SL, Staudinger J, Olson EN, Rubin CS (1998) Structure, expression, and properties of an atypical protein kinase C (PKC3) from Caenorhabditis elegans. PKC3 is required for the normal progression of embryogenesis and viability of the organism. J Biol Chem 273:1130–1143

    Article  CAS  Google Scholar 

  32. Church DL, Lambie EJ (2003) The promotion of gonadal cell divisions by the Caenorhabditis elegans TRPM cation channel GON-2 is antagonized by GEM-4 copine. Genetics 165:563–574

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Xue Y, Fares H, Grant B, Li Z, Rose AM, Clark SG, Skolnik E (2003) Genetic analysis of the myotubularin family of phosphatases in Caenorhabditis elegans. J Biol Chem 278:34380–34386

    Article  CAS  Google Scholar 

  34. Labrousse AM, Shurland DL, van der Bliek AM (1998) Contribution of the GTPase domain to the subcellular localization of dynamin in the nematode Caenorhabditis elegans. Mol Biol Cell 9:3227–3239

    Article  CAS  Google Scholar 

  35. Ren M-X, Zhao L, Ding X-C, Krasteva N, Rui Q, Wang D-Y (2018) Developmental basis for intestinal barrier against the toxicity of graphene oxide. Part Fibre Toxicol 15:26

    Article  Google Scholar 

  36. Legouis R, Gansmuller A, Sookhareea S, Bosher JM, Baillie DL, Labouesse M (2000) LET-413 is a basolateral protein required for the assembly of adherens junctions in Caenorhabditis elegans. Nat Cell Biol 2:415–422

    Article  CAS  Google Scholar 

  37. Culetto E, Sattelle DB (2000) A role for Caenorhabditis elegans in understanding the function and interactions of human disease genes. Hum Mol Genet 9:869–977

    Article  CAS  Google Scholar 

  38. Altun ZF, Chen B, Wang ZW, Hall DH (2009) High resolution map of Caenorhabditis elegans gap junction proteins. Dev Dyn 238:1936–1950

    Article  CAS  Google Scholar 

  39. Saifee O, Wei LP, Nonet ML (1998) The Caenorhabditis elegans unc-64 locus encodes a syntaxin that interacts genetically with synaptobrevin. Mol Biol Cell 9:1235–1252

    Article  CAS  Google Scholar 

  40. Segbert C, Johnson K, Theres C, van Furden D, Bossinger O (2004) Molecular and functional analysis of apical junction formation in the gut epithelium of Caenorhabditis elegans. Dev Biol 266:17–26

    Article  CAS  Google Scholar 

  41. Koppen M, Simske JS, Sims PA, Firestein BL, Hall DH, Radice AD, Rongo C, Hardin JD (2001) Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nat Cell Biol 3:983–991

    Article  Google Scholar 

  42. Lackner MR, Nurrish SJ, Kaplan JM (1999) Facilitation of synaptic transmission by EGL-30 Gqα and EGL-8 PLCβ: DAG binding to UNC-13 is required to stimulate acetylcholine release. Neuron 24:335–346

    Article  CAS  Google Scholar 

  43. Feng W, Long JF, Fan JS, Suetake T, Zhang M (2004) The tetrameric L27 domain complex as an organization platform for supramolecular assemblies. Nat Struct Mol Biol 11:475–480

    Article  CAS  Google Scholar 

  44. Wu Q-L, Rui Q, He K-W, Shen L-L, Wang D-Y (2010) UNC-64 and RIC-4, the plasma membrane associated SNAREs syntaxin and SNAP-25, regulate fat storage in nematode Caenorhabditis elegans. Neurosci Bull 26:104–116

    Article  CAS  Google Scholar 

  45. Tabuse Y, Izumi Y, Piano F, Kemphues KJ, Miwa J, Ohno S (1998) Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans. Development 125:3607–3614

    CAS  PubMed  Google Scholar 

  46. Lee I, Lehner B, Crombie C, Wong W, Fraser AG, Marcotte EM (2008) A single gene network accurately predicts phenotypic effects of gene perturbation in Caenorhabditis elegans. Nat Genet 40:181–188

    Article  CAS  Google Scholar 

  47. Ziegler K, Kurz CL, Cypowyj S, Couillault C, Pophillat M, Pujol N, Ewbank JJ (2009) Antifungal innate immunity in C. elegans: PKCdelta links G protein signaling and a conserved p38 MAPK cascade. Cell Host Microbe 5:341–352

    Article  CAS  Google Scholar 

  48. Beatty A, Morton D, Kemphues K (2010) The C. elegans homolog of Drosophila lethal giant larvae functions redundantly with PAR-2 to maintain polarity in the early embryo. Development 137:3995–4004

    Article  CAS  Google Scholar 

  49. Galli M, Munoz J, Portegijs V, Boxem M, Grill SW, Heck AJ, van den Heuvel S (2011) aPKC phosphorylates NuMA-related LIN-5 to position the mitotic spindle during asymmetric division. Nat Cell Biol 13:1132–1138

    Article  CAS  Google Scholar 

  50. Armenti ST, Chan E, Nance J (2014) Polarized exocyst-mediated vesicle fusion directs intracellular lumenogenesis within the C. elegans excretory cell. Dev Biol 394:110–1121

    Article  CAS  Google Scholar 

  51. Kang J, Shin D, Yu JR, Lee J (2009) Lats kinase is involved in the intestinal apical membrane integrity in the nematode Caenorhabditis elegans. Development 136:2705–2815

    Article  CAS  Google Scholar 

  52. MacQueen AJ, Baggett JJ, Perumov N, Bauer RA, Januszewski T, Schriefer L, Waddle JA (2005) ACT-5 is an essential Caenorhabditis elegans actin required for intestinal microvilli formation. Mol Biol Cell 16:3247–3259

    Article  CAS  Google Scholar 

  53. Szumowski SC, Estes KA, Popovlch JJ, Botts MR, Sek G, Troemel ER (2016) Small GTPases promote actin coat formation on microsporidian pathogens traversing the apical membrane of Caenorhabditis elegans intestinal cells. Cell Microbiol 18:30–45

    Article  CAS  Google Scholar 

  54. Zhi L-T, Yu Y-L, Li X-Y, Wang D-Y, Wang D-Y (2017) Molecular control of innate immune response to Pseudomonas aeruginosa infection by intestinal let-7 in Caenorhabditis elegans. PLoS Pathog 13:e1006152

    Article  Google Scholar 

  55. Sun L-M, Liao K, Hong C-C, Wang D-Y (2017) Honokiol induces reactive oxygen species-mediated apoptosis in Candida albicans through mitochondrial dysfunction. PLoS One 12:e0172228

    Article  Google Scholar 

  56. Sun L-M, Liao K, Wang D-Y (2017) Honokiol induces superoxide production by targeting mitochondrial respiratory chain complex I in Candida albicans. PLoS One 12:e0184003

    Article  Google Scholar 

  57. Sun L-M, Liao K, Li Y-P, Zhao L, Liang S, Guo D, Hu J, Wang D-Y (2016) Synergy between PVP-coated silver nanoparticles and azole antifungal against drug-resistant Candida albicans. J Nanosci Nanotechnol 16:2325–2335

    Article  CAS  Google Scholar 

  58. Estes KA, Szumowski SC, Troemel ER (2011) Non-lytic, actin-based exit of intracellular parasites from C. elegans intestinal cells. PLoS Pathog 7:e1002227

    Article  CAS  Google Scholar 

  59. Qu M, Xu K-N, Li Y-H, Wong G, Wang D-Y (2018) Using acs-22 mutant Caenorhabditis elegans to detect the toxicity of nanopolystyrene particles. Sci Total Environ 643:119–126

    Article  CAS  Google Scholar 

  60. Shao H-M, Han Z-Y, Krasteva N, Wang D-Y (2018) Identification of signaling cascade in the insulin signaling pathway in response to nanopolystyrene particles. Nanotoxicology. https://doi.org/10.1080/17435390.2018.1530395

  61. Dong S-S, Qu M, Rui Q, Wang D-Y (2018) Combinational effect of titanium dioxide nanoparticles and nanopolystyrene particles at environmentally relevant concentrations on nematodes Caenorhabditis elegans. Ecotoxicol Environ Saf 161:444–450

    Article  CAS  Google Scholar 

  62. Zhi L-T, Fu W, Wang X, Wang D-Y (2016) ACS-22, a protein homologous to mammalian fatty acid transport protein 4, is essential for the control of toxicity and translocation of multi-walled carbon nanotubes in Caenorhabditis elegans. RSC Adv 6:4151–4159

    Article  CAS  Google Scholar 

  63. Zhao Y-L, Yu X-M, Jia R-H, Yang R-L, Rui Q, Wang D-Y (2015) Lactic acid bacteria protects Caenorhabditis elegans from toxicity of graphene oxide by maintaining normal intestinal permeability under different genetic backgrounds. Sci Rep 5:17233

    Article  CAS  Google Scholar 

  64. Gelino S, Chang JT, Kumsta C, She X, Davis A, Nguyen C, Panowski S, Hansen M (2016) Intestinal autophagy improves healthspan and longevity in C. elegans during dietary restriction. PLoS Genet 12:e1006135

    Article  Google Scholar 

  65. Zhao Y-L, Zhi L-T, Wu Q-L, Yu Y-L, Sun Q-Q, Wang D-Y (2016) p38 MAPK-SKN-1/Nrf signaling cascade is required for intestinal barrier against graphene oxide toxicity in Caenorhabditis elegans. Nanotoxicology 10:1469–1479

    Article  CAS  Google Scholar 

  66. Li W-J, Wang D-Y, Wang D-Y (2018) Regulation of the response of Caenorhabditis elegans to simulated microgravity by p38 mitogen-activated protein kinase signaling. Sci Rep 8:857

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Medicine, Southeast University, Nanjing, China

    Dayong Wang

Authors
  1. Dayong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Wang, D. (2019). Intestinal Barrier for Nematodes Against Toxicity of Environmental Toxicants or Stresses. In: Target Organ Toxicology in Caenorhabditis elegans. Springer, Singapore. https://doi.org/10.1007/978-981-13-6010-7_3

Download citation

Publish with us

Policies and ethics

Search

Navigation