Reconstruction and in Silico Simulation Towards Electricigens Metabolic Network of Electronic Mediator

Abstract

Microorganisms have drawn our attention for its distinguished capacity of electron transportation. Recently microbial fuel cells (MFC) have emerged as a novel biochemical catalytic device, the electron transfer mechanisms are of our primary concern to improve the efficiency of extracellular electron transfer (EET). Here we reconstruct the genome-scale metabolic network of Shewanella oneidensis MR-1 by merlin. Based on text mining, we focus on the electronic mediators, then we complete the reconstruction of the metabolic sub-network. We have identified the key reactions of EET process combined with the utilization of COBRA Toolbox, the simulated gene and reaction knockouts provided guidance to the strain genetic modification to a certain level.