Abstract
A comparative study of the neutralisation of the indirect immunofluorescence (NIIF) test, the immunodiffusion (ID) tes, enzyme-linked immunosorbent assay (ELISA) and electron microscopy (EM) for the detection of rotaviruses in calf faeces was done at the Nationaal Instituut voor Diergeneeskundig Onderzoek (NIDO) in Belgium and the Centraal Diergeneeskundig Instituut (CDI) in The Netherlands. A total of 93 faecal samples was examined. Sixty-two per cent was positive for rotavirus by EM, 59% with the NIIF test, 58% with the ID test and 57% with ELISA. All samples negative by EM were also negative in the NIIF and the ID test but three of these samples were found positive in the ELISA.
Forty-three faecal samples were tested for the presence of bovine Coronavirus. Eleven samples were scored positive by EM of which ten were positive in the NIIF test and five in ELISA. Two other samples were found positive by ELISA only.
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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Van Opdenbosch, E., Wellemans, G., Ellens, D.J., De Leeuw, P.W. (1981). The Neutralisation of the Indirect Immunofluorescence Test and the Immunodiffusion Test: Two Detection Methods for Neonatal Calf Diarrhoea Viruses. A Comparative Study with Elisa and Electron Microscopy. In: de Leeuw, P.W., Guinée, P.A.M. (eds) Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea. Current Topics in Veterinary Medicine and Animal Science, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8328-1_7
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DOI: https://doi.org/10.1007/978-94-009-8328-1_7
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