Abstract
A fiber-optic system for detection of in vivo fluorescence was developed. Exciting argon laser light and fluorescence are transmitted through the same fiber using appropriate micro-optic devices (dichroic mirrors, filters, selfoc lenses), an optional spectral analyzer and an avalanche photodetector. This system allows for optimum flexibility, remote sensing and detection of small areas in the submillimeter range of biological samples. Preliminary measurements include the induction and decrease of chlorophyll fluorescence during light exposure of intact spruce needles and needles submitted to water stress.
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References
Bader J, 1988. Aufbau und Optimierung eines faseroptischen Sensors zur Messung schwacher Fluoreszenzsignale. Diplomarbeit, Fachhochschule Aalen.
Krause H, Gerhardt V and Gebhardt W, 1984. Verzögerte Fluoreszenz lebender Pflanzenzellen. Phys. unserer Zeit 15: 182–190.
Lichtenthaler HK, Buschmann C, Rinderle U and Schmuck G, 1986. Application of Chlorophyll fluorescence in ecophysiology. Radiat. Environ. Biophys. 25: 297–308.
Scheper I and Schügerl K, 1986. Characterization of bioreactors by in-situ fluorometry. J. Biotechnol. 3: 224–229.
Schneckenburger H, Reuter BW and Schoberth SM, 1985. Fluorescence techniques in biotechnology. Trends Biotechnol. 3: 257–261.
Schneckenburger H and Frenz M, 1986. Time-resolved fluorescence of conifers exposed to environmental pollutants. Radiat. Environ. Biophys. 25: 289–295.
Schneckenburger H, Frenz M, Tsuchiya Y, Denzer U and Schleinkofer L, 1987. Picosecond fluorescence microscopy for measuring chlorophyll and porphyrin components in conifers and cultured cells. Lasers Life Sci. 1: 299–307.
Schreiber U, 1986. Differenzierung zwischen verschiedenen Typen der Fluoreszenzlöschung und Bestimmung der relativen Elektronen-Transportrate in vivo mit Hilfe der Sättigungspuls-Methode. Symp. Chlorophyll Fluorescence in Ecology, Hannover (FRG).
Schulze E, Pätzold G, Röther-Steinke M and Ernst D, 1983. Chlorophyllfluoreszenz zur Früherkennung von Photosythesebeeinträchtigungen an Coniferen. Forstarchiv 54: 229–232.
Wolfbeis OS, 1986. Analytical chemistry with optical sensors. Fresenius Z. Anal. Chem. 325: 387–392.
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© 1988 Kluwer Academic Publishers
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Schneckenburger, H., Bader, J. (1988). Fiber — Optic Detection of Chlorophyll Fluorescence. In: Lichtenthaler, H.K. (eds) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2823-7_32
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DOI: https://doi.org/10.1007/978-94-009-2823-7_32
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7771-2
Online ISBN: 978-94-009-2823-7
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