Abstract
This paper reports the effect of pH on the adsorption characteristics of fungal polygalacturonase in CM-Sephadex ion exchangers, in dilute solutions and in batch operations. The partition coefficient increases sharply at pH below 4.3, reaching a peak at about pH 3.2. Equilibrium isotherms follow the Langmuir law and are strongly affected by pH; the maximum adsorption capacity of adsorbent increases with decreasing pH. These findings reflect the effect of pH on the relative degrees of ionisation of adsorbate and adsorbent, and highlight the need to determine the optimum pH for every specific enzyme system.
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Harsa, S., Pyle, D.L., Zaror, C.A. (1990). Effect of pH on Polygalacturonase Adsorption in CM-Sephadex Gels. In: Pyle, D.L. (eds) Separations for Biotechnology 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0783-6_37
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DOI: https://doi.org/10.1007/978-94-009-0783-6_37
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6839-0
Online ISBN: 978-94-009-0783-6
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