Skip to main content

Reactivation of Dark-Inactivated RuBP Carboxylase from Phaseolus vulgaris in vitro

  • Chapter
  • 12 Accesses

Abstract

It has been proposed that light regulates the activity of RuBP carboxylase by two mechanisms. One is through Activase which has been shown to control the level of carbamylation of enzyme thus controlling the level of activation (1). The other mechanism involves binding of a phosphorylated inhibitor viz 2-carboxy-arabinitol 1-phosphate to the activated form of enzyme at low light intensities and in the dark. Thus after binding with this inhibitor, RuBP carboxylase remains in activated form but is unable to perform catalytic function (2,3,4). This inhibitor along with activase has been shown to match the activity of RuBP carboxylase with RuBP regenerating capacity in vivo (5).

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

CA1P:

2-carboxy-D-arabinitol 1-phosphate

R-5-P:

Ribose-5-phosphate

RuBP:

Ribulose-1 5-bisphosphate

References

  1. Portis, A.R., Salvucci, M.E. and Ogren, W.L. (1986) Plant Physiol. 82, 967–971.

    Article  PubMed  CAS  Google Scholar 

  2. Servaites, J.C. (1985) Plant Physiol. 78, 839–843.

    Article  PubMed  CAS  Google Scholar 

  3. Seemann, J.R., Berry, J.A., Freas, S.M. and Krump, M.A. (1985) Proc. Natl. Acad. Sci. USA, 82, 8024–8028.

    Article  PubMed  CAS  Google Scholar 

  4. Gutteridge, S., Parry, M.A.J., Burton, S., Keys, A.J., Mudd, A., Feeney, J., Servaites, J.C. and Pierce, J. (1986) Nature. 324, 274–276.

    Article  CAS  Google Scholar 

  5. Kobza, J., Seemann, J.R. (1989) Plant Physiol. 89, 174–179.

    Article  PubMed  CAS  Google Scholar 

  6. Sainis, J.K. and Harris, G.C. (1986) Biochem. Biophys. Res. Commun. 139, 947–954.

    Article  PubMed  CAS  Google Scholar 

  7. Sainis, J.K. and Harris, G.C. (1987) Prog. Photosynth. Res. 6, 491–494.

    Article  Google Scholar 

  8. Sainis, J.K., Merriam, K. and Harris, G.C. (1989) Plant Physiol. 89, 368–374.

    Article  PubMed  CAS  Google Scholar 

  9. Gontero, B., Cardenas, M.L. and Ricard, J. (1988) Eur. J. Biochem. 173, 437–443.

    Article  PubMed  CAS  Google Scholar 

  10. Berry, J.A., Lorimer, G.H., Pierce, J., Seemann, J.R., Meek, J. and Freas, S. (1987) Proc. Natl. Acad. Sci. USA. 84, 734–738.

    Article  PubMed  CAS  Google Scholar 

  11. Hall, N.P. and Tolbert, N.E.(1978) FEBS Lett. 96, 167–169.

    Article  CAS  Google Scholar 

  12. Robinson, S.P. and Portis, A.R. (1988) FEBS Lett. 233, 413–416.

    Article  CAS  Google Scholar 

  13. Salvucci, M.E., Holbrook, G.P., Anderson, J.C. and Bowes, G. (1988) FEBS Lett. 231, 197–201.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer Science+Business Media New York

About this chapter

Cite this chapter

Sainis, J.K., Jawali, N. (1990). Reactivation of Dark-Inactivated RuBP Carboxylase from Phaseolus vulgaris in vitro . In: Baltscheffsky, M. (eds) Current Research in Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0511-5_529

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-0511-5_529

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6716-4

  • Online ISBN: 978-94-009-0511-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics