Abstract
Fresh tissue/blood samples provide the best sources of DNA for biological analyses, but their availability is often limited. Although cryopreservation (freezing) has proved to be the most effective method for preserving various tissues, the most routine and popular ways for preserving animal samples are to use alcohol, formalin or buffered solutions as preservatives. To study population biology, systematics and biodiversity at the molecular level, it may be of great interest in many cases to have some historical knowledge about the species or populations in question; and researchers sometimes need to re-examine previous studies. Furthermore, sample collection for many species, or populations, may not be possible at a given time because of difficulties and costs due to remoteness, population decline or even extinction.
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References
Walsh PS, Metzger DA, Higuchi R (1991). Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10: 507–513
Greer CE, Wheeler CM, Manos MM (1994). Sample preparation and PCR amplification from paraffin-embedded tissues. PCR Methods and Applications 4: s113–s122
Tan AM, Orrego C (1992). DNA stabilization and amplification from museum collections of extracts originally intended for allozyme analysis. Molecular Ecology 1: 195–197
Pääbo S (1990). ‘Amplifying ancient DNA’ In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, pp 159–166
Hagelberg E, Clegg JB (1991). Isolation and characterization of DNA from archaeological bone. Proceedings of the Royal Society of London, B 244: 45–50
Poinar HN, Poinar GO, Cano RJ (1992). Extracting DNA from amber embedded organisms. Ancient DNA Newsletter 1 (2): 26–27
Higuchi R, vonBeroldigen CH, Sensabaugh GF, Erlich HA (1988) DNA typing from single hairs. Nature 332: 543–546
Wilson MR, Polanskey D, Butler J, DiZinno JA, Replogle J, Budowle B (1995). Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts. BioTechniques 18: 662–669
Höss M, Kohn M, Pääbo S, Knauer F, Schröder W (1992) Excrement analysis by PCR. Nature 359: 199
Hänni C, Brousseau T, Laudet V, Stehelin D (1995). Isopropanol precipitation removes PCR inhibitors from ancient bone extracts. Nucleic Acids Research 23: 881–882
Dubeau L, Chandler LA, Gralow JR, Nichols PW, Jones PA (1986). Southern blot analysis of DNA extracted from formalin-fixed pathology specimens. Cancer Research 46: 2964–2969
Jackson DP, Hayden JD, Quirke P (1991) ‘Extraction of nucleic acid from fresh and archival material’ In: McPherson MJ, Quirke P, Taylor GR (eds) PCR: A Practical Approach. IRL Press at Oxford University Press, Oxford, pp29–50
Santos AC, Osorio-Almeida L (1993). Simultaneous extraction of RNA and DNA from paraffin-embedded tissues. Trends in Genetics 9:231
Premoli-de-Percoco G, Guevara P, Galindo I, Ramirez JL (1994). Rapid recovery of DNA from paraffin-embedded tissue sections for routine Polymerase Chain Reaction analysis. Methods in Molecular and Cellular Biology 4: 266–268.
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Zhang, DX., Hewitt, G.M. (1998). Isolation of DNA from Preserved Specimens. In: Karp, A., Isaac, P.G., Ingram, D.S. (eds) Molecular Tools for Screening Biodiversity. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0019-6_9
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DOI: https://doi.org/10.1007/978-94-009-0019-6_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6496-5
Online ISBN: 978-94-009-0019-6
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