Skip to main content

Use of Recombinant Viruses to Manipulate Neural Stem Cell Gene Expression in the Mouse Brain

  • Chapter
  • First Online:
  • 1199 Accesses

Part of the book series: Stem Cells and Cancer Stem Cells ((STEM,volume 7))

Abstract

Adult mammalian brain preserves neural stem cells (NSCs) that contribute to postnatal neurogenesis. The last decade has seen a tremendous progress in the identification and analysis of NSCs in the mouse brain. A handful of molecular and cell biology techniques have been applied to confirm the existence and to address the functions of NSCs in the adult brain. Recombinant viruses constitute one of the most powerful and versatile tools for the analysis of NSCs in vivo. This chapter describes major recombinant viruses that are presently available and discusses their applicability to the NSC analysis.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Alonso M, Ortega-Perez I, Grubb MS, Bourgeois JP, Charneau P, Lledo PM (2008) Turning astrocytes from the rostral migratory stream into neurons: a role for the olfactory sensory organ. J Neurosci 28:11089–11102

    Article  CAS  PubMed  Google Scholar 

  • Beckervordersandforth R, Tripathi P, Ninkovic J, Bayam E, Lepier A, Stempfhuber B, Kirchhoff F, Hirrlinger J, Haslinger A, Lie DC, Beckers J, Yoder B, Irmler M, Gotz M (2010) In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells. Cell Stem Cell 7:744–758

    Article  CAS  PubMed  Google Scholar 

  • Bonaguidi MA, Wheeler MA, Shapiro JS, Stadel RP, Sun GJ, Ming GL, Song H (2011) In vivo clonal analysis reveals self-renewing and multipotent adult neural stem cell characteristics. Cell 145:1142–1155

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK (1993) Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90:8033–8037

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Colak D, Mori T, Brill MS, Pfeifer A, Falk S, Deng C, Monteiro R, Mummery C, Sommer L, Gotz M (2008) Adult neurogenesis requires Smad4-mediated bone morphogenic protein signaling in stem cells. J Neurosci 28:434–446

    Article  CAS  PubMed  Google Scholar 

  • Fernandez ME, Croce S, Boutin C, Cremer H, Raineteau O (2011) Targeted electroporation of defined lateral ventricular walls: a novel and rapid method to study fate specification during postnatal forebrain neurogenesis. Neural Dev 6:13

    Article  PubMed Central  PubMed  Google Scholar 

  • Hermonat PL, Quirk JG, Bishop BM, Han L (1997) The packaging capacity of adeno-associated virus (AAV) and the potential for wild-type-plus AAV gene therapy vectors. FEBS Lett 407:78–84

    Article  CAS  PubMed  Google Scholar 

  • Jackson EL, Garcia-Verdugo JM, Gil-Perotin S, Roy M, Quinones-Hinojosa A, VandenBerg S, Alvarez-Buylla A (2006) PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 51:187–199

    Article  CAS  PubMed  Google Scholar 

  • Jacquet BV, Patel M, Iyengar M, Liang H, Therit B, Salinas-Mondragon R, Lai C, Olsen JC, Anton ES, Ghashghaei HT (2009) Analysis of neuronal proliferation, migration and differentiation in the postnatal brain using equine infectious anemia virus-based lentiviral vectors. Gene Ther 16:1021–1033

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jang JH, Koerber JT, Kim JS, Asuri P, Vazin T, Bartel M, Keung A, Kwon I, Park KI, Schaffer DV (2011) An evolved adeno-associated viral variant enhances gene delivery and gene targeting in neural stem cells. Mol Ther 19:667–675

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Khodosevich K, Monyer H (2011) Signaling in migrating neurons: from molecules to networks. Front Neurosci 5:28

    Article  PubMed Central  PubMed  Google Scholar 

  • Khodosevich K, Seeburg PH, Monyer H (2009) Major signaling pathways in migrating neuroblasts. Front Mol Neurosci 2:7

    Article  PubMed Central  PubMed  Google Scholar 

  • Khodosevich K, Watanabe Y, Monyer H (2011) EphA4 preserves postnatal and adult neural stem cells in an undifferentiated state in vivo. J Cell Sci 124:1268–1279

    Article  CAS  PubMed  Google Scholar 

  • Koerber JT, Klimczak R, Jang JH, Dalkara D, Flannery JG, Schaffer DV (2009) Molecular evolution of adeno-associated virus for enhanced glial gene delivery. Mol Ther 17:2088–2095

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kuwabara T, Hsieh J, Nakashima K, Taira K, Gage FH (2004) A small modulatory dsRNA specifies the fate of adult neural stem cells. Cell 116:779–793

    Article  CAS  PubMed  Google Scholar 

  • Lai K, Kaspar BK, Gage FH, Schaffer DV (2003) Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo. Nat Neurosci 6:21–27

    Article  CAS  PubMed  Google Scholar 

  • Lawlor PA, Bland RJ, Mouravlev A, Young D, During MJ (2009) Efficient gene delivery and selective transduction of glial cells in the mammalian brain by AAV serotypes isolated from nonhuman primates. Mol Ther 17:1692–1702

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lim DA, Tramontin AD, Trevejo JM, Herrera DG, Garcia-Verdugo JM, Alvarez-Buylla A (2000) Noggin antagonizes BMP signaling to create a niche for adult neurogenesis. Neuron 28:713–726

    Article  CAS  PubMed  Google Scholar 

  • Liu G, Martins IH, Chiorini JA, Davidson BL (2005a) Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS. Gene Ther 12:1503–1508

    Article  CAS  PubMed  Google Scholar 

  • Liu X, Wang Q, Haydar TF, Bordey A (2005b) Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors. Nat Neurosci 8:1179–1187

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lledo PM, Alonso M, Grubb MS (2006) Adult neurogenesis and functional plasticity in neuronal circuits. Nat Rev Neurosci 7:179–193

    Article  CAS  PubMed  Google Scholar 

  • Lugert S, Basak O, Knuckles P, Haussler U, Fabel K, Gotz M, Haas CA, Kempermann G, Taylor V, Giachino C (2011) Quiescent and active hippocampal neural stem cells with distinct morphologies respond selectively to physiological and pathological stimuli and aging. Cell Stem Cell 6:445–456

    Article  Google Scholar 

  • Mao Y, Ge X, Frank CL, Madison JM, Koehler AN, Doud MK, Tassa C, Berry EM, Soda T, Singh KK, Biechele T, Petryshen TL, Moon RT, Haggarty SJ, Tsai LH (2009) Disrupted in schizophrenia 1 regulates neuronal progenitor proliferation via modulation of GSK3beta/beta-catenin signaling. Cell 136:1017–1031

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Miller DG, Adam MA, Miller AD (1990) Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol Cell Biol 10:4239–4242

    CAS  PubMed Central  PubMed  Google Scholar 

  • Ming GL, Song H (2011) Adult neurogenesis in the mammalian brain: significant answers and significant questions. Neuron 70:687–702

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Morshead CM, Craig CG, van der Kooy D (1998) In vivo clonal analyses reveal the properties of endogenous neural stem cell proliferation in the adult mammalian forebrain. Development 125:2251–2261

    CAS  PubMed  Google Scholar 

  • Stein CS, Martins I, Davidson BL (2005) The lymphocytic choriomeningitis virus envelope glycoprotein targets lentiviral gene transfer vector to neural progenitors in the murine brain. Mol Ther 11:382–389

    Article  CAS  PubMed  Google Scholar 

  • Suh H, Consiglio A, Ray J, Sawai T, D’Amour KA, Gage FH (2007) In vivo fate analysis reveals the multipotent and self-renewal capacities of Sox2+ neural stem cells in the adult hippocampus. Cell Stem Cell 1:515–528

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhang R, Zhang Z, Wang L, Wang Y, Gousev A, Zhang L, Ho KL, Morshead C, Chopp M (2004) Activated neural stem cells contribute to stroke-induced neurogenesis and neuroblast migration toward the infarct boundary in adult rats. J Cereb Blood Flow Metab 24:441–448

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Konstantin Khodosevich .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Khodosevich, K. (2012). Use of Recombinant Viruses to Manipulate Neural Stem Cell Gene Expression in the Mouse Brain. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 7. Stem Cells and Cancer Stem Cells, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4285-7_23

Download citation

Publish with us

Policies and ethics