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Toxins and Hemostasis pp 167–177Cite as

Blood Coagulation Factor IX/Factor X-Binding Protein

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Abstract

C-type lectin-like proteins of snake have a variety of biological properties, acting for example as an anticoagulant, procoagulant, and agonist/antagonist of platelet activation. Dimerization or oligomer formation of carbohydrate recognition domain (CRD) in C-type lectin by 3D domain swapping generates novel proteins with new functions such as coagulant-, anticoagulant-, and platelet-modulating activities. The structural and functional studies of the first identified C-type lectin-like proteins, IX/X-bp, have been instrumental in defining how new functionally heterodimeric C-type lectin-like proteins are generated from monomeric CRD (carbohydrate recognition domain) in C-type lectin by 3D domain swapping. The crystal structure of IX/X-bp revealed that the two subunits associated by 3 D-domain swapping, and this dimerization resulted in the creation of a concave surface serving as a binding site of Gla domain, the functionally important domain of blood coagulation factors. The strong activities by snaclecs such as IX/X-bp and X-bp are caused by the binding at the Gla domain of factors IX and X. C-type lectin-like proteins of snake venom such as IX/X-bp and its structurally-related proteins recognize various ligands by the higher frequency of mutation in the open reading frames than in the non-coding regions after duplication of a gene.

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Authors and Affiliations

  1. Department of Biochemistry, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo, 204-0004, Japan

    Takashi Morita

  2. Department of Host Defense and Biological Research, Juntendo University School of Medicine, Tokyo, 113-8421, Japan

    Takashi Morita

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  1. Takashi Morita
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Correspondence to Takashi Morita .

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Editors and Affiliations

  1. , Department of Biological Sciences, National University of Singapore, Science Drive 4 14, Singapore, 117543, Singapore

    R. Manjunatha Kini

  2. , Department of Haematology, University of Berne, Inselspital, Bern, CH-3010, Switzerland

    Kenneth J Clemetson

  3. , Biochemistry and Molecular Biology, University of Southern California, Health Sciences Campus/9270, Los Angeles, 90089, USA

    Francis S. Markland

  4. , Medical Technology, University of Delaware, 305G Willard Hall Educ Bld, Newark, 19716, Delaware, USA

    Mary Ann McLane

  5. , Biochemistry, Meiji Pharmaceutical University, Nishio Kiyose 2-522-1, Tokyo, 204-8588, Japan

    Takashi Morita

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Morita, T. (2010). Blood Coagulation Factor IX/Factor X-Binding Protein. In: Kini, R., Clemetson, K., Markland, F., McLane, M., Morita, T. (eds) Toxins and Hemostasis. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9295-3_11

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