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Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989–1990 U.S. epizootic

  • Conference paper
Imported Virus Infections

Part of the book series: Archives of Virology Supplement II ((ARCHIVES SUPPL,volume 11))

Summary

This study describes the pathogenesis of the Ebola-Reston (EBO-R) subtype of Ebola virus for experimentally infected cynomolgus monkeys. The disease course of EBO-R in macaques was very similar to human disease and to experimental diseases in macaques following EBO-Zaire and EBO-Sudan infections. Cynomolgus monkeys infected with EBO-R in this experiment developed anorexia, occasional nasal discharge, and splenomegaly, petechial facial hemorrhages and severe subcutaneous hemorrhages in venipuncture sites, similar to human Ebola fever. Five of the six EBO-R infected monkeys died, 8 to 14 days after inoculation. One survived and developed high titered neutralizing antibodies specific for EBO-R. The five acutely ill monkeys shed infectious virus in various bodily secretions. Further, abundant virus was visualized in alveolar interstitial cells and free in the alveoli suggesting the potential for generating infectious aerosols. Thus, taking precautions against aerosol exposures to filovirus infected primates, including humans, seems prudent. This experiment demonstrated that EBO-R was lethal for macaques and was capable of initiating and sustaining the monkey epizootic. Further investigation of this animal model should facilitate development of effective immunization, treatment, and control strategies for Ebola hemorrhagic fever.

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Author information

Author notes

  1. Dr. P. B. Jahrling (Scientific Advisor)

    Present address: Headquarters, Ft. Detrick, USAMRIID, Frederick, MD, 21702-5011, USA

  2. T. G. Ksiazek & C. J. Peters

    Present address: Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia, USA

Authors and Affiliations

  1. Headquarters, Ft. Detrick, USAMRIID, Frederick, MD, 21702-5011, USA

    T. W. Geisbert, N. K. Jaax, M. A. Hanes, T. G. Ksiazek & C. J. Peters

Authors
  1. Dr. P. B. Jahrling
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  2. T. W. Geisbert
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  3. N. K. Jaax
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  4. M. A. Hanes
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  5. T. G. Ksiazek
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  6. C. J. Peters
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Editor information

Editors and Affiliations

  1. Stiftung Juliusspital, Würzburg, Federal Republic of Germany

    Tino F. Schwarz

  2. Institute for Clinical Microbiology and Immunology, St. Gallen, Switzerland

    Günter Siegl

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© 1996 Springer-Verlag/Wien

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Jahrling, P.B., Geisbert, T.W., Jaax, N.K., Hanes, M.A., Ksiazek, T.G., Peters, C.J. (1996). Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989–1990 U.S. epizootic. In: Schwarz, T.F., Siegl, G. (eds) Imported Virus Infections. Archives of Virology Supplement II, vol 11. Springer, Vienna. https://doi.org/10.1007/978-3-7091-7482-1_11

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  • DOI: https://doi.org/10.1007/978-3-7091-7482-1_11

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82829-8

  • Online ISBN: 978-3-7091-7482-1

  • eBook Packages: Springer Book Archive

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