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Testing for Genetic Manipulation in Plants pp 1–17Cite as

Selectable and Screenable Markers for Rice Transformation

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Part of the book series: Molecular Methods of Plant Analysis ((MOLMETHPLANT,volume 22))

Abstract

Rice transformation is a major goal in cereal biotechnology, not only because rice is the world’s most important food crop but also because this species has now been recognized as the model for cereal genomics. A number of alternative transformation strategies are available, the most widely used of which are particle bombardment (Christou et al. 1991) and Agrobacterium-mediated transformation (Hiei et al. 1994). Regardless of the chosen strategy, transformation is a low-efficiency process. Since most foreign genes introduced into plants do not confer a phenotype that can be used conveniently for the identification or selective propagation of transformed cells, marker genes must therefore be introduced along with the transgene of interest to provide such a phenotype.

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  1. R. M. Twyman
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  2. E. Stöger
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  3. A. Kohli
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  4. T. Capell
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  5. P. Christou
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Editors and Affiliations

  1. Department of Horticulture, Viticulture and Oenology, University of Adelaide, Waite Campus, SA 5064, Glen Osmond, Australia

    J. F. Jackson

  2. Goldberglein 7, 91056, Erlangen, Germany

    H. F. Linskens

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Twyman, R.M., Stöger, E., Kohli, A., Capell, T., Christou, P. (2002). Selectable and Screenable Markers for Rice Transformation. In: Jackson, J.F., Linskens, H.F. (eds) Testing for Genetic Manipulation in Plants. Molecular Methods of Plant Analysis, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04904-4_1

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  • DOI: https://doi.org/10.1007/978-3-662-04904-4_1

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  • Print ISBN: 978-3-642-07730-2

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