Abstract
The diminishing petroleum reserves in the world call for sustainable use of cheaply and readily available substrates such as wastewater streams for biomass and lipid production by microalgae. Treated wastewater is rich in macronutrients, such as nitrates and phosphates, and can therefore be used as a substrate for microalgal cultivation in open raceway ponds. The chemistry and composition of treated wastewater is of significance since it is made up of a wide range of compounds that support microalgal growth. The use of raceway pond technology utilizing wastewater streams feed is a new phenomenon that provides much needed phytoremediation of the wastewater as well as facilitating microalgal mass production. Macronutrient utilization by the microalgae justifies the application of treated wastewater as a sustainable raw material for renewable bioenergy production. The operational parameters in the raceway pond such as light intensity, photoperiod, pH, nutrients, salinity, and temperature are carefully optimized for maximal biomass and lipid yield. The biomass and lipid produced using the raceway pond system undergoes downstream processing in order to get the final product. The lipids are converted via transesterification to produce algae biodiesel. Other biologically active compounds and novel phytochemicals can also be derived from microalgae.
Keywords
- Lipid Production
- Supercritical Fluid Extraction
- Transesterification Reaction
- Microalgal Biomass
- Lipid Yield
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Anandraj A, Perissinotto R, Nozais C (2008) The recovery of microalgal production and biomass in a South African temporarily—open/closed estuary, following mouth breaching. Estuar Coast Shelf Sci 79:599–606
Anandraj A, Perissinotto R, Nozais C (2007) A comparative study of microalgal production in a marine versus a river-dominated temporarily open/closed estuary, South Africa. Estuar Coast Shelf Sci 73:768–780
Bare WFR, Jones NB, Middlebrooks EJ (1975) Algae removal using dissolved air flotation. J Water Pollut Control 47(1):153–169
Beardall J, Young E, Roberts S (2001) Approaches for determining phytoplankton nutrient limitation. Aquat Sci 63:44–69
Borowitzka MA (2005) Culturing microalgae in outdoor ponds. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, UK
Moser Bryan R (2009) Biodiesel production, properties, and feedstocks. In Vitro Cell. Dev Biol Plant 45:229–266. doi:10.1007/s11627-009-9204-z
Camacho Rubio F, García Camacho F, Fernández Sevilla JM, Chisti Y, Molina Grima E (2003) A mechanistic model of photosynthesis in microalgae. Biotechnol Bioeng 81:459–473
Chen W, Zhang Q, Dai S (2009) Effects of nitrate on intracellular nitrite and growth of Microcystis aeruginosa. J Appl Phycol 21:701–706
Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25(3):294–306
Costa JAV, Colla LM, Filho PD (2003) Spirulina platensis growth in open raceway ponds using fresh water supplemented with carbon, nitrogen and metal ions. Z Naturforsch 58:76–80
Craggs RJ, McAuley PJ, Smith VJ (1997) Wastewater nutrient removal by marine microalgae grown on a corrugated raceway. Water Res 31(7):1701–1707
de-Bashan LE, Moreno M, Hernandez JP, Bashaan Y (2002) Removal of ammonium and phosphorus ions from synthetic wastewater by the microalgae Chlorella vulgaris coimmobilised in alginate beads with the microalgae growth-promoting bacterium Azospirillum brasilense. Water Res 36:2941–2948
Demirbas A (1998) Fuel properties and calculation of higher heating values of vegetable oils. Fuel 77:1117–1120
Demirbas A (2002) Biodiesel from vegetable oils via transesterification in supercritical methanol. Energy Convers Manage 43:2349–2356
Demirbas A (2003) Biodiesel fuels from vegetable oils via catalytic and non catalytic supercritical alcohol transesterifications and other methods: a survey. Energ Convers Manage 44:2093–2109. doi:10.1016/S0196-8904(02)00234-0
Demirbas A (2008) Production of biodiesel from tall oil. Energy Sour Part A 30:1896–1902
Demirbas A (2009) Production of biodiesel from algae oils. Energy Sour Part A 31:163–168. doi:10.1080/15567030701521775
Desmorieux H, Decaen N (2006) Convective drying of spirulina in thin layer. J Food Eng 66(4):497–503
Du W, Xu Y, Liu D, Zeng J (2004) Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors. J Mol Catal B Enzymat 30:125–129
Eyster C (1958) Chloride Effect on the Growth of Chlorella pyrenoidosa. Nature 181:1141–1142
Falkowski PG, Wyman K, Ley AC, Mauzerall DC (1986) Relationship of steady-state photosynthesis to fluorescence in eucaryotic algae. Biochim Biophys Acta 849:183–192
Folkman Y, Wachs AM (1970) Filtration of Chlorella through Dune-Sand. Proc Am Soc Civil Eng, J Sanit Eng Div 96:675–690
Freedman B, Butterfield RO, Pryde EH (1986) Transesterification kinetics of soybean oil. JAOCS 63:1375–1380
Furuta S, Matsuhashi H, Arata K (2004) Biodiesel fuel production with solid super acid catalysis in fixed bed reactor under atmospheric pressure. Catal Commun 5:721–723
Gernaey KV, van Loosdrecht MCM, Henze M, Lind M, Jorgensen SB (2004) Activated sludge wastewater treatment plant modelling and simulation: state of the art. Environ Model Softw 19:763–783
Green diesel (2009) http://www.green-diesel.co.za/info_standards.htm. Accessed 20 Oct 2009
Grima (1994) Comparison between extraction of lipids and fatty acids from microalgal biomass. JAOCS 71(9):955–959
Grima ME, Belarbi EH, Fernandez FGA, Medina AR, Chisti Y (2003) Recovery of microalgal biomass and metabolites: process options and economics. Biotechnol Adv 20(7–8):491–515
Grima ME, Acién Fernández FG, García Camacho F, Camacho Rubio F, Chisti Y (2000) Scale-up of tubular photobioreactors. J Appl Phycol 12:355–368
Grobbelaar JU (2007) Photosynthetic characteristics of Spirulina platensis grown in commercial-scale open outdoor raceway ponds: what do the organisms tell us? J Appl Phycol 19:591–598
Gryglewicz S (1999) Rapeseed oil methyl esters preparation using heterogeneous catalysts. Bioresour Technol 70:249–253
Gudin C, Therpenier C (1986) Bioconversion of solar energy into organic chemicals by microalgae. Adv Biotechnol Process 6:73–110
Hama S, Yamaji H, Kaieda M, Oda M, Kondo A, Fukuda H (2004) Effect of fatty acid membrane composition on whole-cell biocatalysts for biodiesel-fuel production. Biochem Eng J 21:155–160
Harrison PJ, Berges JA (2005) Marine culture media. In: Andersen RA (ed) Algal culturing techniques. Elservier Academic press, London
Hsieh CH, Wu WT (2009) Cultivation of microalgae for oil production with a cultivation strategy of urea limitation. Bioresour Technol 100:3921–3926
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639
Huber GW, Iborra S, Corma A (2006) Synthesis of transportation fuels from biomass: chemistry, catalysts, and engineering. Chem Rev 106:4044–4098
Kaplan D, Richmond AE, Dubinsky Z, Aaronson S (1986) Algal Nutrition. In: Richmond A (ed) Handbook of microalgal mass culture. CRC Press Inc, USA
Knothe G, Steidley KR (2005) Kinematic viscosity of biodiesel fuel components and related compounds. Influence of compound structure and comparison to petrodiesel fuel components. Fuel 84:1059–1065. doi:10.1016/j.fuel.2005.01.016
Knothe G, Van Gerpen J, Krahl J (2005) The biodiesel handbook. AOCS, Urbana
Kolber Z, Falkowski PG (1993) Use of active fluorescence to estimate phytoplankton photosynthesis in situ. Limnol Oceanogr 38:1646–1665
Kong Q, Li L, Martinez B, Chen P, Ruan R (2010) Culture of microalgae Chlamydomonas reinhardtii in wastewater for biomass feedstock production. Appl Biochem Biotechnol 160:9–18
Koopman B, Lincoln EP (1983) Autoflotation of algae from high-rate pond effluents. Agric Wastes 5(4):231–246
Krawczyk T (1996) Biodiesel—alternative fuel makes inroads but hurdles remain. Inform 7:801–829
Kromkamp JC, Forster RM (2003) The use of variable fluorescence measurements in aquatic ecosystems: differences between multiple and single turnover measuring protocols and suggested terminology. Eur J Phycol 38:103–112
Kromkamp J, Peene J (1999) Estimation of phytoplankton photosynthesis and nutrient limitation in the Eastern Scheldt estuary using variable fluorescence. Aquat Ecol 33:101–104
Chung Kyong Hwan, Kim Jin, Lee Ki-Young (2009) Biodiesel production by transesterification of duck tallow with methanol on alkali catalysts. Biomass Bioenergy 33(1):155–158
Leach G, Oliveira G, Morais R (1998) Spray-drying of Dunaliella salina to produce abcarotene rich powder. J Ind Microbiol Biotechnol 20(2):82–85
Li Y, Horsman M, Wu N, Lan CQ, Dubois-Calero N (2008) Biofuels from microalgae. Biotech Prog 24(4):815–820
Li Y, Horsman M, Wang B, Wu N, Lan CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol 81:629–636
Lima SAC, Raposo MFJ, Castro PML, Morais RM (2004) Biodegradation of p-chlorophenol by a microalgae consortium. Water Res 38:97–102
Ma F, Hanna MA (1999) Biodiesel production: a review. Bioresour Technol 70:1–15
MacIntyre HL, Cullen JJ (1996) Primary production by suspended and benthic microalgae in a turbid estuary: time-scales of variability in San Antonio Bay. Tex Mar Ecol Prog Ser 145:245–268
Marchetti JM, Miguel VU, Errazu AF (2007) Possible methods for biodiesel production. Renew Sustain Energy Rev 11:1300–1311
Masojidek J, Koblizek M, Torzillo G (2004) Photosynthesis in microalgae. In: Richmond A (ed) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Science Ltd, Oxford, pp 20–39
Meher LC, Vidya SD, Naik SN (2006) Technical aspects of biodiesel production by transesterification—a review. Renew Sust Energy Rev 10:248–268
Mohn FH (1988) Harvesting of micro-algal biomass. In: Borowitzka LJ, Borowitzka MA (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, pp 395–414
Muga HE, Mihelcic JR (2008) Sustainability of wastewater treatment technologies. J Environ Manage 88:437–447
Mulaku WO, Nyanchanga EN (2004) Dissolved air flotation process for algae removal in surface water treatment in Kenya. J Civil Eng Res Pract 1(2):27–38
Mulbry W, Kondrad S, Buyer J (2008) Treatment of dairy and swine manure effluents using freshwater algae: fatty acid content and composition of algal biomass at different manure loading rates. J Appl Phycol 20:1079–1085
Mutanda T, Karthikeyan S, Mustapha S, Bux F (2010) The utilisation of post-chlorinated municipal domestic wastewater for biomass and lipid production by Chlorella sp. under batch conditions. Biomass Bioenergy (in press)
Nindo CI, Tang J (2007) Refractance window dehydration technology: a novel contact drying method. Dry Technol 25:37–48
Noue J, Laliberte G, Proulx D (1992) Algae and wastewater. J Appl Phycol 4:247–254
Noureddini H, Gao X, Philkana RS (2005) Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil. Bioresour Technol 96:769–777
Nurdogan Y, Oswald WJ (1996) Tube settling rate of high-rate pond algae. Water Sci Technol 33:229–241
Oda M, Kaieda M, Hama S, Yamaji H, Kondo A, Izumoto E, Fukuda H (2004) Facilitatory effect of immobilized lipase-producing Rhizopus oryzae cells on acyl migration in biodiesel-fuel production. Biochem Eng J 23:45–51
Olivier S, Scragg AH, Morrison J (2003) The effect of chlorophenols on the growth of Chlorella VT-1. Enzym Microb Technol 32:837–842
Oswald WJ, Lee EW, Adan B, Yao KH (1978) New wastewater treatment method yields a harvest of saleable algae. WHO Chron 32:348–350
Paul PFM, Wise WS (1971) The principle of gas extraction. Mills Boon, London
Prakash J, Pushparaj B, Carlozzi P, Torzillo G, Montaini E, Materassi R (1997) Microalgal biomass drying by a simple solar device. Int J Solar Energy 18(4):303–311
Pushparaj B, Pelosi E, Tredici MR, Pinzani E, Materassi R (1997) An integrated culture system for outdoor production of microalgae and cyanobacteria. J Appl Phycol 9:113–119
Ramdhani N, Bux F (2007) Functional characterization of heterotrophic denitrifying bacteria in activated sludge. S Afr J Sci 103:113–116
Ramesh D, Samapathrajan A, Venkatachalam P (2005) Pilot biodiesel plant for vegetable oils. Periyar J Res Dev 3(1):15–19
Ramesh D, Samapathrajan A, Joshua Davidson S (2005) Fuel properties of palm oil and its biodiesel production. Periyar J Res Dev 2(3):25–29
Reid EE (1911) Studies in esterification. IV. The interdependence of limits as exemplified in the transformation of esters. Am Chem J 45:479–516
Republic of South Africa (1998) National Water Act. Act No 36 of 1998
Richmond A (2004) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Science Ltd, Oxford
Ryll T, Dutina G, Reyes A, Gunson J, Krummen L, Etcheverry T (2000) Performance of small-scale CHO perfusion cultures using an acoustic cell filtration device for cell retention: Characterization of separation efficiency and impact of perfusion on product quality. Biotechnol Bioengng 69:440–449
Schreiber U (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth Res 9:261–272
Serodio J, Vieira S, Cruz S, Barroso F (2005) Short-term variability in the photosynthetic activity of microphytobenthos as detected by measuring rapid light curves using variable fluorescence. Mar Biol 146:903–914
Sheehan J, Camobreco V, Duffield J, Graboski M, Shapouri H (1998) Life cycle inventory of biodiesel and petroleum diesel for use in an urban Bus. Final Report NREL/SR-580-24089. National Renewable Energy Laboratory, Golden, Colorado
Shelef GA, Sukenik A, Green M (1984) Microalgae harvesting and processing: a literature review report. Solar Energy Research Institute, Golden Colorado, SERI/STR-231-2396
Shieh CJ, Liao HF, Lee CC (2003) Optimization of lipase-catalyzed biodiesel by response surface methodology. Bioresour Technol 88:103–106
Simmons MS, Sivaborvorn K (1979) Effects of chlorinated organics from wastewater treatment on algal growth. Bull Environ Contam Toxicol 23:766–773
Sidat M, Kasan HC, Bux F (1999) Laboratory scale investigation of biological phosphate removal from municipal wastewater. Water SA 25(4):459–462
Srivastava A, Prasad R (2000) Triglycerides-based diesel fuels. J Renew Sustain Energy Rev 4:111–133
Teixeira MR, Rosa MJ (2006) Comparing dissolved air flotation and conventional sedimentation to remove cyanobacterial cells of Microcystis aeruginosa. Part 1: the key operating conditions. Sep Purif Technol 52(1):84–94
Torzillo G, Bernardini P, Masojidek J (1998) On-line monitoring of chlorophyll fluorescence to assess the extent of photoinhibition of photosynthesis induced by high oxygen concentration and low temperature and its effects on the productivity of outdoor cultures of Spirulina platensis (Cyanobacteria). J Phycol 34:504–510
Valderramma LT, Campo CMD, Rodriguez CM, de-Bashan LE, Bashan Y (2002) Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemma minuscule. Water Res 36:4185–4192
Van Gerpen J, Shanks B, Pruszko R, Clements D and Knothe G (2004) Biodiesel production technology. National Renewable Energy Laboratory. 1617 Cole Boulevard, Golden, CO. Paper contract No. DE-AC36-99-GO10337
Venkataraman LV (1978) New possibility for microalgae production and utilisation in India. Arch Hydrobiol Beih 11:199–210
Vonshak A (1997) Spirulina: growth, physiology and biochemisty. In: Vonshak A (ed) Spirulina platensis (Arthrospira): physiology, cell-biology and biochemistry. Taylor & Francis, London, pp 43–65
Wang B, Li Y, Wu N, Lan CQ (2008) CO2 bio-mitigation using microalgae. Appl Microbiol Biotechnol 79(5):707–718
Widjaja A, Chien CC, Ju YH (2009) Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. J Taiwan Inst Chem Eng 40(1):13–20
Wijffels RH (2007) Potential of sponges and microalgae for marine biotechnology. Trends Biotechnol 26(1):26–31
Miao Xiaoling, Li Rongxiu, Yao Hongyan (2009) Effective acid-catalyzed transesterification for biodiesel production. Energy Convers Manage 50(10):2680–2684
Xu H, Miao XL, Wu QY (2006) High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol 126:499–507
Zhang Y, Dub MA, McLean DD, Kates M (2003) Biodiesel production from waste cooking oil: 2. Economic assessment and sensitivity analysis. Bioresour Technol 90:229–240
Zhou W, Boocock DBG (2006) Phase behavior of the base-catalyzed transesterification of soybean oil. JAOCS 83:1041–1045. doi:10.1007/s11746-006-5160-5
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Mutanda, T., Ramesh, D., Anandraj, A., Bux, F. (2013). Sustainable Biodiesel Production Using Wastewater Streams and Microalgae in South Africa. In: Gurib-Fakim, A., Eloff, J. (eds) Chemistry for Sustainable Development in Africa. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29642-0_4
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