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Plant Electrophysiology pp 91–126Cite as

Application of Non-invasive Microelectrode Flux Measurements in Plant Stress Physiology

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Abstract

Non-invasive microelectrode flux measurement (the MIFE™ technique) is a convenient tool to study membrane-transport processes in plants in situ. Over the last 20 years, many papers have been published elucidating the critical role of membrane-transport processes in response to a variety of abiotic and biotic stresses including salinity, osmotic stress, temperature extremes, acidity, oxygen deprivation, nutritional disorders, oxidative stress, and pathogens and elicitors. In this review, we summarize some of these findings and illustrate how the application of ion-selective microelectrodes may be combined with other techniques to address some fundamental issues related to mechanisms of plant nutrient acquisition and stress signaling and adaptation.

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Abbreviations

abi1 :

Abscisic acid-insensitive1

akt1 :

Arabidopsis K+ transporter1

ALMT:

Aluminum-activated malate transporter

AM:

Arbuscular mycorrhiza

AtCNGC10:

Arabidopsis cyclic nucleotide-gated channel10

AtHELPS :

Arabidopsis DExD/H box RNA helicase

ATP:

Adenosine triposphate

CED-9:

Cell death defective-9

Cu/a:

Cu2+/ascorbate

[Ca2+]cyt :

Cytosolic free calcium

Em:

Membrane potential

GORK:

Guard cell outward-rectifying K+ channel

HR:

Hypersensitive response

Kcv:

Viral-encoded K+ channel

KIR:

K+ inward-rectifying channel

KOR:

K+ outward-rectifying channel

LIX:

Liquid ion exchanger

MATE:

Multidrug and toxic efflux transporter

MBS:

Marine bioactive substances

NORC:

Nonspecific outward-rectifying channel

NSCC:

Non-selective cation channel

O2 :

Oxygen

PAMPs:

Pathogen-associated molecular patterns

PA:

Polyamines

PBCV-1:

Paramecium bursaria chlorella virus

PCD:

Programmed cell death

PM:

Plasma membrane

PMV:

Papaya mosaic virus

PVX:

Potato virus X

ROS:

Reactive oxygen species

SAC:

Stretch-activated channels

SIET:

Scanning ion-selective electrode technique

TEA:

Tetraethylammonium

TUNEL:

Terminal deoxynucleotidyl dUTP nick end labeling

ucu2-2/gi2:

ultracurvata2-2/gigantea-2

UV-C:

Ultraviolet-C

zVAD-fmk:

Benzyloxycarbonyl-Val-Ala-Asp (OMe)—uoromethylketone

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Acknowledgements

This work was supported by the Grain Research and Development Corporation and Australian Research Council grants to Prof Sergey Shabala. The authors are grateful to Dr Ian Newman for his critical reading and valuable suggestions on this manuscript.

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  1. School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, TAS, 7001, Australia

    Sergey Shabala & Jayakumar Bose

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  1. Sergey Shabala
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  2. Jayakumar Bose
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Correspondence to Sergey Shabala .

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Editors and Affiliations

  1. , Department of Chemistry, Oakwood University, Adventist Blvd. 7000, Huntsville, 35896, Alabama, USA

    Alexander G. Volkov

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© 2012 Springer-Verlag Berlin Heidelberg

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Shabala, S., Bose, J. (2012). Application of Non-invasive Microelectrode Flux Measurements in Plant Stress Physiology. In: Volkov, A. (eds) Plant Electrophysiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29119-7_4

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