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Oral Cavity and Oropharynx

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Nongynecologic Cytopathology

Part of the book series: Essentials of Diagnostic Pathology ((ESSENTPATH))

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Abstract

Performance of brushing and processing of the obtained cell material are key sections in this chapter. Specific interest is directed towards (1) cytologic evaluation of leukoplakia, and (2) the impact of DNA-ploidy measurement in order to ascertain the malignant potential of abnormal squamous cells.

Diagnostic algorithms of benign and malignant findings in cytologic specimens and respective differential diagnoses are additionally presented in a synoptic setup.

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Further Reading

  1. Banoczy J, Rigo O. Comparative cytologic and histologic studies in oral leukoplakia. Acta Cytol 1976;20:308-312.

    PubMed  CAS  Google Scholar 

  2. Baretton G, Li X, Stoll C, et al. Prognostic significance of DNA ploidy in oral squamous cell carcinomas. A retrospective flow and image cytometric study with comparison of DNA ploidy in excisional biopsy specimens and resection specimens, primary tumors and lymph node metastases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995;79:68-76.

    Article  PubMed  CAS  Google Scholar 

  3. Barnes L, Eveson JW, Reichart P, Sidransky D. World Health Organization Classification of Tumours. Pathology and Genetics of Head and Neck Tumours. IARC Press: Lyon 2005.

    Google Scholar 

  4. Batsakis JG, Suarez P, El Naggar AK. Proliferative verrucous leukoplakia and its related lesions. Oral Oncol 1999;35:354-359.

    Article  PubMed  CAS  Google Scholar 

  5. Blot WJ, McLaughlin JK, Winn DM, et al. Smoking and drinking in relation to oral and pharyngeal cancer. Cancer Res 1988;48:3282-3287.

    PubMed  CAS  Google Scholar 

  6. Bremmer JF, Braakhuis BJ, Ruijter-Schippers HJ, et al. A noninvasive genetic screening test to detect oral preneoplastic lesions. Lab Invest 2005;85:1481-1488.

    PubMed  CAS  Google Scholar 

  7. Chattopadhyay A, Ray JG, Caplan DJ. AgNOR count as objective marker for dysplastic features in oral leukoplakia. J Oral Pathol Med 2002;31:512-517.

    Article  PubMed  Google Scholar 

  8. Chow TL, Chow TK, Lui YH, et al. Lymphoepithelioma-like carcinoma of oral cavity: report of three cases and literature review. Int J Oral Maxillofac Surg 2002;31:212-218.

    Article  PubMed  CAS  Google Scholar 

  9. Christian DC. Computer-assisted analysis of oral brush biopsies at an oral cancer screening program. J Am Dent Assoc 2002;133:357-362.

    PubMed  Google Scholar 

  10. Dabelsteen E, Roed-Petersen B, Smith CJ, Pindborg JJ. The limitation of exfoliative cytology for the detection of epithelial atypia in oral leukoplakias. Br J Cancer 1971;25:21-24.

    Article  PubMed  CAS  Google Scholar 

  11. Daskalopoulou D, Rapidis AD, Maounis N, Markidou S. Fineneedle aspiration cytology in tumors and tumor-like conditions of the oral and maxillofacial region: diagnostic reliability and limitations. Cancer 1997;81:238-252.

    Article  PubMed  CAS  Google Scholar 

  12. Diversi HL, Griffin JW, Payne T. Correlation of cytologic nuclear changes to anemias. Oral Surg Oral med Oral Pathol 1966;21:341-346.

    Article  PubMed  CAS  Google Scholar 

  13. Driemel O, Dahse R, Hakim SG, et al. Laminin-5 immunocytochemistry: a new tool for identifying dysplastic cells in oral brush biopsies. Cytopathology 2007;18:348-355.

    Article  PubMed  CAS  Google Scholar 

  14. Driemel O, Kunkel M, Hullmann M, et al. Performance of conventional oral brush biopsies. HNO 2008;56:205-210.

    Article  PubMed  CAS  Google Scholar 

  15. Drinnan AJ. Screening for oral cancer and precancer – a valuable new technique. Gen Dent 2000;48:656-660.

    PubMed  CAS  Google Scholar 

  16. Ferlito A. Primary lymphoepithelial carcinoma of the hypopharynx. J Laryngol Otol 1977;91:361-367.

    Article  PubMed  CAS  Google Scholar 

  17. Frank DK, Cheron F, Cho H, et al. Nonnasopharyngeal lymphoepitheliomas (undifferentiated carcinomas) of the upper aerodigestive tract. Ann Otol Rhinol Laryngol 1995;104:305-310.

    PubMed  CAS  Google Scholar 

  18. Gockel I, Kammerer P, Brieger J, et al. Image cytometric analysis of mucosal biopsies in patients with primary achalasia. World J Gastroenterol 2006;12:3020-3025.

    PubMed  CAS  Google Scholar 

  19. Graham RM, Rheault MH. Characteristic cellular changes in epithelial cells in pernicious anemia. J Lab Clin Med 1954;43:235-245.

    PubMed  CAS  Google Scholar 

  20. Grayson R. Oral cancer, early detection by cytology report of 1000 cases from a group of dentists in New Jersey. Acta Cytol 1969;13:502-506.

    PubMed  CAS  Google Scholar 

  21. Günhan Ö, Dogan N, Celasun B, et al. Fine needle aspiration cytology of oral cavity and jaw bone lesions. A report of 102 cases. Acta Cytol 1993;37:135-141.

    PubMed  Google Scholar 

  22. Handschel J, Oz D, Pomjanski N, et al. Additional use of DNA cytometry improves the assessment of resection margins. J Oral Pathol Med 2007;36:472-475.

    Article  PubMed  Google Scholar 

  23. Haroske G, Baak JP, Danielsen H, Giroud F, Gschwendtner A, Oberholzer M, Reith A, Spieler P, Böcking A. Fourth updated ES-ACP consensus report on diagnostic DNA image cytometry. Anal Cell Pathol 2001;23:89-95.

    PubMed  CAS  Google Scholar 

  24. Hayama FH, Motta AC, Silva Ade P, Maigliari DA. Liquid-based preparations versus conventional cytology: specimen adequacy and diagnostic agreement in oral lesions. Med Oral Patol Oral Cir Bucal 2005;10:115-122.

    PubMed  Google Scholar 

  25. Herrero R, Castellsagué X, Pawlita M, et al. The viral etiology of oral cancer: evidence from the IARC multicentric-study. J Natl Cancer Inst 2003:47-51.

    Google Scholar 

  26. Hirshberg A, Yarom N, Amariglio N, et al. Detection of non-diploid cells in premalignant and malignant oral lesions using combined morphological and FISH analysis - a new method for early detection of suspicious oral lesions. Cancer Lett 2007;253:282-290.

    Article  PubMed  CAS  Google Scholar 

  27. Höfken F, Welkoborsky HJ, Jacob R, Mann W. Examination with DNA cytometry of mouth and oropharyngeal lavage fluid as a screening method in diagnosis of malignancies of the mouth cavity and oropharynx. Laryngorhinootologie 1995;74:678-683.

    Article  PubMed  Google Scholar 

  28. Högmo A, Lindskog S, Lindholm J, et al. Preneoplastic oral lesions: the clinical value of image cytometry DNA analysis, p53 and p21/WAF1 expression. Anticancer Res 1998;18(5B):3645-3650.

    PubMed  Google Scholar 

  29. Hullmann M, Reichert TE, Dahse R, et al. Oral cytology: historical development, current status, and perspectives. Mund Kiefer Gesichtschirurgie 2007;11:1-9.

    Article  CAS  Google Scholar 

  30. Itakura Y. Sasano H, Mori S, Nagura H. DNA ploidy in human esophageal squamous dysplasias and squamous cell carcinomas as determined by image analysis. Mod Pathol 1994;7:867-873.

    PubMed  CAS  Google Scholar 

  31. Jones AC, Pink FE, Sandow PL, et al. The cytobrush Plus cell collector in oral cytology. Oral Surg Oral Med Oral Pathol 1994;77:95-99.

    Article  PubMed  CAS  Google Scholar 

  32. Joseph BK. Oral cancer: prevention and detection. Med Princ Pract 2002;11 Suppl 1:32-35.

    Article  PubMed  Google Scholar 

  33. Junquera LM, de Vicente JC, Vega JA, et al. Granular-cell tumours: an immunohistochemical study. Br J Oral Maxillofac Surg 1997;35:180-184.

    Article  PubMed  CAS  Google Scholar 

  34. Kahn MA, Dockter ME, Hermann-Petrin JM. Proliferative verrucous leukoplakia. Four cases with flow cytometric analysis. Oral Surg Oral Med Oral Pathol 1994;78:469-475.

    Article  PubMed  CAS  Google Scholar 

  35. Klanrit P, Sperandio M, Brown AL, et al. DNA ploidy in proliferative verrucous leukoplakia. Oral Oncol 2007;43:310-316.

    Article  PubMed  CAS  Google Scholar 

  36. Kobayashi TK, Kaneko C, Sugishima S, et al. Scrape cytology of oral pemphigus. Report of a case with immunocytochemistry and light, scanning electron and transmission electron microscopy.Acta Cytol 1999;43:289-294.

    Article  PubMed  CAS  Google Scholar 

  37. Koss LG. The oral cavity, larynx, trachea, nasopharynx, and paranasal sinuses (chapter 21), In: Koss’ Diagnostic Cytology, 5th edition. Lippincott Williams & Wilkins 2006; p.714 - 725

    Google Scholar 

  38. Kujan O, Duxbury AJ, Glenny AM, et al. Opinions and attitudes of the UK’s GDPs and specialists in oral surgery, oral medicine and surgical dentistry on oral cancer screening. Oral Dis 2006;12:194-199.

    Article  PubMed  CAS  Google Scholar 

  39. Lingen MW, Kalmar JR, Karrison T, Speight PM. Critical evaluation of diagnostic aids for the detection of oral cancer. Oral Oncol 2008;44:10-22.

    Article  PubMed  Google Scholar 

  40. Maraki D, Becker J, Boecking A. Cytologic and DNA-cytometric very early diagnosis of oral cancer. J Oral Pathol Med 2004;33:398-404.

    Article  PubMed  CAS  Google Scholar 

  41. Maraki D, Boecking A, Pomjanski N, et al. Verrucous carcinoma of the buccal mucosa: histopathological, cytological and DNA-cytometric features. J Oral Pathol Med 2006;35:633-635.

    Article  PubMed  Google Scholar 

  42. Maraki D, Yalcinkaya S, Pomjanski N, et al. Cytologic and DNA-cytometric examination of oral lesions in lichen planus. J Oral Pathol Med 2006;35:227-232.

    Article  PubMed  Google Scholar 

  43. Medak H, Burlakow P, McGrew EA, Tiecke R. The cytology of vesicular conditions affecting the oral mucosa: pemphigus vulgaris. Acta Cytol 1970;14:11-21.

    PubMed  CAS  Google Scholar 

  44. Moralis A, Kunkel M, Reichert TE, et al. Identification of a recurrent oral squamous cell carcinoma by brush cytology. Mund Kiefer Gesichtschir 2007;11:355-358.

    Article  PubMed  Google Scholar 

  45. Nagler RM. Molecular aspects of oral cancer. Anticancer Res 2002;22:2977-2980.

    PubMed  CAS  Google Scholar 

  46. Navone R, Burlo P, Pich A, et al. The impact of liquid-based oral cytology on the diagnosis of oral squamous dysplasia and carcinoma. Cytopathology 2007;18:356-360.

    Article  PubMed  CAS  Google Scholar 

  47. Navone R, Pentenero M, Rostan I, et al. Oral potentially malignant lesions: first-level micro-histological diagnosis from tissue fragments sampled in liquid-based diagnostic cytology. J Oral Pathol Med 2008;37:358-363.

    Article  PubMed  Google Scholar 

  48. Noguchi M, Kinjyo H, Kohama GI, Nakamori K. Invasive front in oral squamous cell carcinoma: Image and flow cytometric analysis with clinicopathologic correlation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:682-687.

    Article  PubMed  Google Scholar 

  49. Noutomi Y, Oga A, Uchida K, et al. Comparative genomic hybridization reveals genetic progression of oral squamous cell carcinoma from dysplasia via two different tumourigenic pathways. J Pathol 2006;210:67-74.

    Article  PubMed  CAS  Google Scholar 

  50. Ogden GR. The future role for oral exfoliative cytology - bleak or bright? Oral Oncol 1997;33:2-4.

    Article  PubMed  CAS  Google Scholar 

  51. Pandit S, Aithal D. A qualitative and quantitative estimation of Ag-NORs in dysplastic and non-dysplastic leukoplakias. Indian J Dent Res 2002;13:27-30.

    PubMed  Google Scholar 

  52. Pektas ZO, Keskin A, Günhan O, Karslioglu Y. Evaluation of nuclear morphometry and DNA ploidy status for detection of malignant and premalignant oral lesions: quantitative cytologic assessment and review of methods for cytomorphometric measurements. Oral Maxillofac Surg 2006;64:628-635.

    Article  Google Scholar 

  53. Pereira MC, Oliveira DT, Landman G, Kowalski LP. Histologic subtypes of oral squamous cell carcinoma: prognostic relevance. J Can Dent Assoc 2007;73:339-344.

    PubMed  Google Scholar 

  54. Pillai KR, Sujathan K, Madhavan J, Abraham EK. Significance of silver-stained nucleolar organizer regions in early diagnosis and prognosis of oral squamous cell carcinoma: a multivariate analysis. In Vivo 2005;19:807-812.

    PubMed  Google Scholar 

  55. Poate TW, Buchanan JA, Hodgson TA, et al. An audit of the efficacy of the oral brush biopsy technique in a specialist oral medicine unit. Oral Oncol 2004;40:829-834.

    Article  PubMed  CAS  Google Scholar 

  56. Potter TJ, Summerlin DJ, Campbell JH. Oral malignancies associated with negative transepithelial brush biopsy J Oral Maxillofac Surg 2003;61:674-677.

    Article  PubMed  Google Scholar 

  57. Ramaesh T, Ratnatunga N, Mendis BR, Rajapaksa S. Exfoliative cytology in screening for malignant and premalignant lesions in the buccal mucosa. Ceylon Med J 1998;43:206-209.

    PubMed  CAS  Google Scholar 

  58. Remmerbach TW, Weidenbach H, Hemprich A, Böcking A. Earliest detection of oral cancer using non-invasive brush biopsy including DNA-image-cytometry: report on four cases. Anal Cell Pathol 2003;25:159-166.

    PubMed  CAS  Google Scholar 

  59. Remmerbach TW, Weidenbach H, Müller C, et al. Diagnostic value of nucleolar organizer regions (AgNORs) in brush biopsies of suspicious lesions of the oral cavity. Anal Cell Pathol 2003;25:139-146.

    PubMed  CAS  Google Scholar 

  60. Remmerbach TW, Weidenbach H, Pomjanski N et al. Cytologic and DNA-cytometry early diagnosis of oral cancer. Anal Cell Pathol 2001;22:211-221.

    PubMed  CAS  Google Scholar 

  61. Ries JC, Schreiner D, Steininger H, Girod SC. p53 mutation and detection of p53 protein expression in oral leukoplakia and oral squamous cell carcinoma.Anticancer Res 1998;18(3B):2031-2036.

    PubMed  CAS  Google Scholar 

  62. Rosin MP, Lam WL, Poh C, et al. 3p14 and 9p21 loss is a simple tool for predicting second oral malignancy at previously treated oral cancer sites. Cancer Res 2002;62:6447-6450.

    PubMed  CAS  Google Scholar 

  63. Ruocco V, Coscia-Porrazzi L, Pisani M. Reliability of cytodiagnosis in oral pemphigus vulgaris. A study of 30 cases. J Dermatol 1984;11:535-540.

    PubMed  CAS  Google Scholar 

  64. Saito T, Mizuno S, Notani K, et al. Flow cytometric analysis of cell cycle fractions in oral leukoplakia. Int J Oral Maxillofac Surg 1998;27:217-221.

    Article  PubMed  CAS  Google Scholar 

  65. Saito T, Yamashita T, Notani K, et al. Flow cytometric analysis of nuclear DNA content in oral leukoplakia: relation to clinicopathologic findings. Int J Oral Maxillofac Surg 1995;24:44-47.

    Article  PubMed  CAS  Google Scholar 

  66. Scheifele C, Schlechte H, Bethke G, Reichart PA. Detection of TP53-mutations in brush biopsies from oral leukoplakias. Mund Kiefer Gesichtschir 2002;6:410-414.

    Article  PubMed  CAS  Google Scholar 

  67. Schulte EK, Joos U, Kasper M, Eckert HM. Cytological detection of epithelial dysplasia in the oral mucosa using Feulgen-DNA-image cytometry. Diagn Cytopathol 1991;7:436-441.

    Article  PubMed  CAS  Google Scholar 

  68. Sciubba JJ. Improving detection of precancerous and cancerous oral lesions. Computer-assisted analysis of the oral brush biopsy. U.S. Collaborative Oral CDx Study Group. J Am Dent Assoc 1999;130:1445-1457.

    PubMed  CAS  Google Scholar 

  69. Scott IS, Odell E, Chatrath P, et al. A minimally invasive immunocytochemical approach to early detection of oral squamous cell carcinoma and dysplasia. Br J Cancer 2006;94:1170-1175.

    Article  PubMed  CAS  Google Scholar 

  70. Seoane J, Asenjo JA, Bascones A, et al. Flow cytometric DNA ploidy analysis of oral cancer comparison with histologic grading. Oral Oncol 1999;35:266-272.

    Article  PubMed  CAS  Google Scholar 

  71. Seoane J, Bascones A, Asenjo JA, et al. Flow cytometric analysis of nuclear DNA content in oral leukoplakia. Clin Otolaryngol Allied Sci 1998;23:136-140.

    Article  PubMed  CAS  Google Scholar 

  72. Shah RM, Nirmal NJ, Doshi JJ, Bilimoria KF. Cyto-diagnosis of oral pemphigus vulgaris. J Oral Med 1982;37:98-101.

    PubMed  CAS  Google Scholar 

  73. Silverman S Jr. Early diagnosis of oral cancer . Cancer 1988;62:1796-1799.

    Article  PubMed  Google Scholar 

  74. Silverman S, Bilimoria KF, Bhargava K, et al. Cytologic, histologic and clinical correlations of precancerous and cancerous oral lesions in 57,518 industrial workers of Gujarat, India. Acta Cytol 1977;21:196-198.

    Google Scholar 

  75. Staats OJ, Robinson LH, Butterworth CE Jr. The effect of systemic therapy on nuclear size of oral epithelial cells in folate-related anemias. Acta Cytol 1969;13:84.

    PubMed  CAS  Google Scholar 

  76. Sudbo J, Bryne M, Johannessen AC, et al. Comparison of histological grading and large-scale genomic status (DNA ploidy) as prognostic tool in oral dysplasia. J Pathol 2001;194:303-310.

    Article  PubMed  CAS  Google Scholar 

  77. Sudbo J, Ried T, Bryne M, et al. Abnormal DNA content predicts the occurrence of carcinomas in non-dysplastic oral white patches. Oral Oncol 2001;37:558-565.

    Article  PubMed  CAS  Google Scholar 

  78. Suen KC, et al. The Papanicolaou Society of Cytopathology Task Force on standards of practice. Guidelines of the Papanicolaou Society of Cytopathology for fine-needle aspiration procedure and reporting. Modern Pathol 1997;10:739-747.

    Google Scholar 

  79. Suter VGA, Morger R, Altermatt HJ, Spieler P, Bornstein MM. Oral erythroplakia and erythroleukoplakia: red and red-white epithelial precursor lesions of the oral mucosa. – part 1: Epidemiology, etiology, histopathology and differential diagnosis. Schweiz Monatschr Zahnmed 2008;118:1-8

    Google Scholar 

  80. Suter VGA, Morger R, Altermatt HJ, Spieler P, Bornstein MM. Oral erythroplakia and erythroleukoplakia: red and red-white epithelial precursor lesions of the oral mucosa. – part 2: cytopathology, pathogenesis, therapy, and prognostic aspects. Schweiz Monatsschr Zahnmed 2008;118:510-518.

    PubMed  Google Scholar 

  81. Tytor M, Wingren S, Olofsson J. Heterogeneity of squamous cell carcinomas of the oral cavity studied by flow cytometry. Pathol Res Pract 1991;187:30-35.

    Article  PubMed  CAS  Google Scholar 

  82. Ugras S, Demirtas I, Bekerecioglu M, et al. Immunohistochemical study on histogenesis of congenital epulis and review of the literature. Pathol Int 1997;47:627-632.

    Article  PubMed  CAS  Google Scholar 

  83. Waldron CA, Shafer WG. Leukoplakia revisited. A clinico-pathologic study of 3256 oral leukoplakias. Cancer 1975;36:1386.

    Article  PubMed  CAS  Google Scholar 

  84. Weir JC, Davenport WD, Skinner RL. A diagnostic and epidemiologic survey of 15,783 oral lesions. JAm DentAssoc 1987;115:439-442.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institut für Pathologie, Kantonsspital St. Gallen, Rorschacherstraße 95, 9007, St. Gallen, Switzerland

    Dr. Peter Spieler

  2. Institut für Klinische Pathologie, UniversitätsSpital Zürich, Schmelzbergstraße 12, 8091, Zürich, Switzerland

    Dr. Matthias Rössle

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  1. Dr. Peter Spieler
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Spieler, P., Rössle, M. (2012). Oral Cavity and Oropharynx. In: Nongynecologic Cytopathology. Essentials of Diagnostic Pathology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-24719-4_8

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  • DOI: https://doi.org/10.1007/978-3-642-24719-4_8

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