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Artificial MicroRNA and Its Applications

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Regulatory RNAs

Abstract

Enhanced understanding of cellular microRNA (miRNA) biogenesis machinery has allowed researchers to engineer synthetic or artificial miRNAs (amiRNAs) that can be designed to direct efficient silencing of any transcript. The amiRNA technology has not only widened the existing gene silencing tool kit but also offers several distinct improvements over existing RNAi approaches, primarily based on siRNA generating hairpin RNA precursors. amiRNAs have already been applied to a wide range of agricultural and medical applications. This chapter discusses various aspects of miRNA processing, design principles of amiRNA expression vectors and their application.

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Acknowledgments

PY is thankful to Council of Scientific and Industrial Research, India, for the Shyama Prasad Mukherjee Fellowship, the award of which enabled PY to develop the amiRNA transgenics, and also to the Management of Ankur Seeds Pvt Ltd. Some part of the research reported here was possible due to a grant awarded by DBT, Govt. of India, to SM.

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Authors and Affiliations

  1. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India

    Sunil Kumar Mukherjee

  2. Research Department, Ankur Seeds Pvt Ltd, Nagpur, MH, India

    Pranjal Yadava

Authors
  1. Pranjal Yadava
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  2. Sunil Kumar Mukherjee
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Correspondence to Sunil Kumar Mukherjee .

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Editors and Affiliations

  1. , New York State Department of Health, Wadsworth Center, New Scotland Avenue 120, New York, 12208, New York, USA

    Bibekanand Mallick

  2. A.J.C. Bose Centenary Building, P-1/12, CIT Scheme - VII M, Bose Institute, Kolkata, 700054, India

    Zhumur Ghosh

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Yadava, P., Mukherjee, S.K. (2012). Artificial MicroRNA and Its Applications. In: Mallick, B., Ghosh, Z. (eds) Regulatory RNAs. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22517-8_20

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