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Metagenomics: Microbial Community Genomes Revealed

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The rapidly expanding field of metagenomics has revolutionized the ability to analyzemicrobial communities by providing access to ‘true’ microbial diversity. This field has a broad range of applications in the areas of biodiversity, systems biology and biotechnology (Handelsman et al. 2002). As most microbial communities in soil, sediment or aquatic environments are highly complex, consisting of hundreds oreven thousands of species of which only a few have been cultured, the approaches collectively described as metagenomics, community genomics or environmental genomics have been developed to help to unlock this thus far hidden diversity. Metagenomics is therefore the application of modern genomic tools used to analyze the collective genomes of whole microbial communities (the metagenome) in an environmental sample, thereby bypassing the need for isolation or cultivation (Béjà et al. 2000; Riesenfeld et al. 2004b). Even though the tip of the microbial ice-berg has barely been scratched, this approach is rapidly increasing our knowledge of microbial genetic and functional diversity through gene/pathway discovery either by sequencing or activity-based screening strategies (Handelsman 2005).

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References

  • Abe T, Sugawara H, Kinouchi M, Kanaya S, Ikemura T (2005) Novel phylogenetic studies of genomic sequence fragments derived from uncultured microbe mixtures in environmental and clinical samples. DNA Res 12:281–290.

    Article  CAS  PubMed  Google Scholar 

  • Abulencia CB, Wyborski DL, Garcia JA, Podar M, Chen W, Chang SH, Chang HW, Watson D, Brodie EL, Hazen TC, Keller M (2006) Environmental whole-genome amplification to access microbial populations in contaminated sediments. Appl Environ Microbiol 72:3291–3301.

    Article  CAS  PubMed  Google Scholar 

  • Allen EE, Banfield JF (2005) Community genomics in microbial ecology and evolution. Nature Rev Microbiol 3:489–498.

    Article  CAS  Google Scholar 

  • Angly FE, Felts B, Breitbart M, Salamon P, Edwards RA, Carlson C, Chan AM, Haynes M, Kelley S, Liu H, Mahaffy JM, Meuller JE, Nulton J, Olson R, Parsons R, Rayhawk S, Suttle CA, Rohwer F (2006) The marine viromes of four oceanic regions. PLoS Biol 4:2121–2131.

    Article  CAS  Google Scholar 

  • Antranikian G, Vorgias CE, Bertoldo C (2005) Extreme environments as a resource for microorganisms and novel biocatalysts. In: LeGal Y, Ulber R (eds) Marine biotechnology I, vol 96. Springer, Berlin, pp 219–262.

    Chapter  Google Scholar 

  • Baker BJ, Tyson GW, Webb RI, Flanagan J, Hugenholtz P, Allen EE, Banfield JF (2006) Lineages of acidophilic archaea revealed by community genomic analysis. Science 314:1933–1935.

    Article  CAS  PubMed  Google Scholar 

  • Béjà O, Aravind L, Koonin EV, Suzuki MT, Hadd A, Nguyen LP, Jovanovich S, Gates CM, Feldman RA, Spudich JL, Spudich EN, DeLong EF (2000) Bacterial rhodopsin: Evidence for a new type of phototrophy in the sea. Science 289:1902–1906.

    Article  PubMed  Google Scholar 

  • Brady SF, Clardy J (2004) Palmitoylputrescine, an antibiotic isolated from the heterologous expression of DNA extracted from bromeliad tank water. J Nat Prod 67:1283–1286.

    Article  CAS  PubMed  Google Scholar 

  • Breitbart M, Felts B, Kelley S, Mahaffy JM, Nulton J, Salamon P, Rohwer. F (2004) Diversity and population structure of a near-shore marine-sediment viral community. Proc R Soc Lond B 271:565–574.

    Article  Google Scholar 

  • Breitbart M, Hewson I, Felts B, Mahaffy JM, Nulton J, Salamon P, Rohwer F (2003) Metagenomic analyses of an uncultured viral community from human feces. J Bacteriol 185:6220–6223.

    Article  CAS  PubMed  Google Scholar 

  • Cavicchioli R, Siddiqui KS, Andrews D, Sowers KR (2002) Low-temperature extremophiles and their applications. Curr Opin Biotechnol 13:253–261.

    Article  CAS  PubMed  Google Scholar 

  • Chen K, Pachter L (2005) Bioinformatics for whole-genome shotgun sequencing of microbial communities. PLoS Comput Biol 1:24e.

    Article  CAS  Google Scholar 

  • Cowan D, Meyer Q, Stafford W, Muyanga S, Cameron R, Wittwer P (2005) Metagenomic gene discovery: past, present and future. Trends Biotechnol 23:321–329.

    Article  CAS  PubMed  Google Scholar 

  • Curtis TP, Sloan WT, Scannell JW (2002) Estimating prokaryotic diversity and its limits. Proc Natl Acad Sci USA 99:10494–10499.

    Article  CAS  PubMed  Google Scholar 

  • D’Amico S, Collins T, Marx JC, Feller G, Gerday C (2006) Psychrophilic microorganisms: challenges for life. EMBO Reports 7:385–389.

    Article  PubMed  CAS  Google Scholar 

  • Daniel R (2004) The soil metagenome—a rich resource for the discovery of novel natural products. Curr Opin Biotechnol 15:199–204.

    Article  CAS  PubMed  Google Scholar 

  • Daniel R (2005) The metagenomics of soil. Nature Rev Microbiol 3:470–478.

    Article  CAS  Google Scholar 

  • DeLong EF (2005) Community genomics in the ocean. Nat Rev Microbiol 3:459–469.

    Article  CAS  PubMed  Google Scholar 

  • DeLong EF, Preston CM, Mincer T, Rich V, Hallam SJ, Frigaard N-U, Martinez A, Sullivan MB, Edwards RA, Brito BR, Chisholm SW, Karl DM (2006) Community genomics among stratified microbial assemblages in the oceans interior. Science 311:496–503.

    Article  CAS  PubMed  Google Scholar 

  • Deutschbauer AM, Chivian D, Arkin AP (2006) Genomics for environmental microbiology. Curr Opin Biotechnol 17:229–235.

    Article  CAS  PubMed  Google Scholar 

  • Edwards R, Rodriguez-Brito R, Wegley L, Haynes M, Breitbart M, Peterson D, Saar M, Alexander S, Alexander Jr E, Rohwer F (2006) Using pyrosequencing to shed light on deep mine microbial ecology. BMC Genomics 7:57.

    Article  PubMed  CAS  Google Scholar 

  • Feller G (2003) Molecular adaptations to cold in psychrophilic enzymes. Cell Mol Life Sci 60:648–662.

    Article  CAS  PubMed  Google Scholar 

  • Ferrer M, Martinez-Abarca F, Golyshin PN (2005) Mining genomes and ‘metagenomes’ for novel catalysts. Curr Opin Biotechnol 16:588–593.

    Article  CAS  PubMed  Google Scholar 

  • Gabor EM, de Vries EJ, Janssen DB (2003) Efficient recovery of environmental DNA for expression cloning by indirect extraction methods. FEMS Microbiol Ecol 44:153–163.

    Article  CAS  PubMed  Google Scholar 

  • Gabor EM, de Vries EJ, Janssen DB (2004) Construction, characterization, and use of small-insert gene banks of DNA isolated from soil and enrichment cultures for the recovery of novel amidases. Environ Microbiol 6:948–958.

    Article  CAS  PubMed  Google Scholar 

  • Gill SR, Pop M, DeBoy RT, Eckburg PB, Turnbaugh PJ, Samuel BS, Gordon JI, Relman DA, Fraser-Liggett CM, Nelson KE (2006) Metagenomic analysis of the human distal gut microbiome. Science 312:1355–1359.

    Article  CAS  PubMed  Google Scholar 

  • Gillespie DE, Brady SF, Bettermann AD, Cianciotto NP, Liles MR, Rondon MR, Clardy J, Goodman RM, Handelsman. J (2002) Isolation of antibiotics turbomycin A and B from a metagenomic library of soil microbial DNA. Appl Environ Microbiol 68:4301–4306.

    Article  CAS  PubMed  Google Scholar 

  • Grant S, Grant WD, Cowan DA, Jones BE, Ma Y, Ventosa A, Heaphy S (2006) Identification of eukaryotic open reading frames in metagenomic cDNA libraries made from environmental samples. Appl Environ Microbiol 72:135–143.

    Article  CAS  PubMed  Google Scholar 

  • Gray KA, Richardson TH, Robertson DE, Swanson RV, Subramanian MV (2003) Soil-based gene discovery: a technology to accelerate and broaden biocatalytic applications. In: Laskin AI, Bennett JW, Gadd GM (eds) Advances in Applied Microbiology, vol 52. Elsevier, USA, pp 1–27.

    Google Scholar 

  • Green BD, Keller M (2006) Capturing the uncultivated majority. Curr Opin Biotechnol 17:236–240.

    Article  CAS  PubMed  Google Scholar 

  • Grzymski JJ, Carter BJ, DeLong EF, Feldman RA, Ghadiri A, Murray AE (2006) Comparative genomics of DNA fragments from six antarctic marine planktonic bacteria. Appl Environ Microbiol 72:1532–1541.

    Article  CAS  PubMed  Google Scholar 

  • Hallam SJ, Putnam NH, Preston CM, Detter JC, Rokhsar D, Richardson PM, DeLong EF (2004) Reverse methanogenesis: testing the hypothesis with environmental genomics. Science 305:1457–1462.

    Article  CAS  PubMed  Google Scholar 

  • Handelsman J (2005) Metagenomics or Megagenomics? Nat Rev Microbiol 3:457–458.

    Article  CAS  Google Scholar 

  • Handelsman J, Liles M, Mann D, Riesenfeld C, Goodman RM (2002) Cloning the metagenome: culture-independent access to the diversity and functions of the uncultivated microbial world. In: Wren B, Dorrell N (eds) Methods in microbiology, functional microbial genomics, vol 33. Academic Press, New York, pp 241–245.

    Chapter  Google Scholar 

  • Hårdeman F, Sjöling S (2007) Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment. FEMS Microbiol Ecol 59:524–534.

    Article  PubMed  Google Scholar 

  • Healy FG, Ray RM, Alrich HC, Wilkie AC, Ingram LO, Shanumugam KT (1995) Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose. Appl Microbiol Biotechnol 43:667–674.

    Article  CAS  PubMed  Google Scholar 

  • Henne A, Daniel R, Schmitz R, Gottschalk G (1999) Construction of environmental DNA libraries in Escherichia coli and screening for the presence of genes conferring utilization of 4-hydroxybutyrate. Appl Environ Microbiol 65:3901–3907.

    CAS  PubMed  Google Scholar 

  • Henne A, Schmitz RA, Bömeke M, Gottschalk G, Daniel R (2000) Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli. Appl Environ Microbiol 66:3113–3116.

    Article  CAS  PubMed  Google Scholar 

  • Knietsch A, Waschkowitz T, Bowien S, Henne A, Daniel. R (2003) Construction and screening of metagenomic libraries derived from enrichment cultures: Generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli. Appl Environ Microbiol 69:1408–1416.

    Article  CAS  PubMed  Google Scholar 

  • Lee CC, Kibblewhite-Accinelli RE, Wagschal K, Robertson GH, Wong DWS (2006a) Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library. Extremophiles 10:295–300.

    Article  CAS  PubMed  Google Scholar 

  • Lee MH, Lee CH, Oh TK, Song JK, Yoon JH (2006b) Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: Evidence for a new family of bacterial lipases. Appl Environ Microbiol 72:7406–7409.

    Article  CAS  PubMed  Google Scholar 

  • Liles MR, Manske BF, Bintrim SB, Handelsman J, Goodman. RM (2003) A census of rRNA genes and linked genomic sequences within a soil metagenomic library. Appl Environ Microbiol 69:2684–2691.

    Article  CAS  PubMed  Google Scholar 

  • Lombardot T, Kottmann R, Pfeffer H, Richter M, Teeling H, Quast C, Glockner FO (2006) Megx.net—database resources for marine ecological genomics. Nucl Acid Res 34:D390–D393.

    Article  CAS  Google Scholar 

  • Lopez-Garcia P, Brochier C, Moreira D, Rodriguez-Valera F (2004) Comparative analysis of a genome fragment of an uncultivated mesopelagic crenarchaeote reveals multiple horizontal gene transfers. Environ Microbiol 6:19–34.

    Article  CAS  PubMed  Google Scholar 

  • Lorentz P, Eck J (2005) Metagenomics and industrial applications. Nat Rev Microbiol 3:510–516.

    Article  CAS  Google Scholar 

  • MacNeil IA, Tiong CL, C. M, August PR, Grossman TH, Loiacono KA, Lynch BA, Phillips T, Narula S, Sundaramoorthi R, Tyler A, Aldredge T, Long H, Gilman M, Holt D, Osburne MS (2001) Expression and isolation of antimicrobial small molecules from soil DNA libraries. J Mol Microbiol Biotechnol 3:301–308.

    CAS  PubMed  Google Scholar 

  • Margesin R, Schinner F (eds) (1999) Biotechnological applications of cold-adapted organisms. Springer, Berlin.

    Google Scholar 

  • Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 31:376–380.

    Google Scholar 

  • Markowitz VM, Ivanova N, Palaniappan K, Szeto E, Korzeniewski F, Lykidis A, Anderson I, Mavrommatis K, Kunin V, Garcia Martin H, Dubchak I, Hugenholtz P, Kyrpides NC (2006) An experimental metagenome data management and analysis system 10.1093/bioinformatics/btl217. Bioinformatics 22:e359–367.

    Article  CAS  PubMed  Google Scholar 

  • Martin HG, Ivanova N, Kunin V, Warnecke F, Barry KW, McHardy AC, Yeates C, He SM, Salamov AA, Szeto E, Dalin E, Putnam NH, Shapiro HJ, Pangilinan JL, Rigoutsos I, Kyrpides NC, Blackall LL, McMahon KD, Hugenholtz P (2006) Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities. Nat Biotechnol 24:1263–1269.

    Article  CAS  Google Scholar 

  • Moreira D, Rodriguez-Valera F, Lopez-Garcia P (2004) Analysis of a genome fragment of a deep-sea uncultivated Group II euryarchaeote containing 16S rDNA, a spectinomycin-like operon and several energy metabolism genes. Environ Microbiol 6:959–969.

    Article  CAS  PubMed  Google Scholar 

  • Moreira D, Rodriguez-Valera F, Lopez-Garcia P (2006) Metagenomic analysis of mesopelagic Antarctic plankton reveals a novel deltaproteobacterial group. Microbiology-SGM 152:505–517.

    Article  CAS  Google Scholar 

  • Noguchi H, Park J, Takagi T (2006) MetaGene: prokaryotic gene finding from environmental genome shotgun sequences. Nucl Acid Res 34:5623–5630.

    Article  CAS  Google Scholar 

  • Podar M, Reysenbach AL (2006) New opportunities revealed by biotechnological explorations of extremophiles. Curr Opin Biotechnol 17:250–255.

    Article  CAS  PubMed  Google Scholar 

  • Quaiser A, Ochsenreiter T, Klenk H, Kletzin A, Treusch A, Meurer G, Eck J, Sensen C, Schleper C (2002) First insight into the genome of an uncultivated crenarchaeote from soil. Environ Microbiol 4:603–611.

    Article  CAS  PubMed  Google Scholar 

  • Ram RJ, Verberkmoes NC, Thelen MP, Tyson GW, Baker BJ, Blake RC, Shah M, Hettich RL, F. BJ (2005) Community proteomics of a natural microbial biofilm. Science 308:1915–1920.

    Article  CAS  PubMed  Google Scholar 

  • Rees H, Grant S, Jones B, Grant W, Heaply S (2003) Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries. Extremophiles 7:415–421.

    Article  CAS  PubMed  Google Scholar 

  • Rhee JK, Ahn DG, Kim YG, Oh JW (2005) New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library. Appl Environ Microbiol 71:817–825.

    Article  CAS  PubMed  Google Scholar 

  • Richardson TH, Tan X, Frey G, Callen W, Cabell M, Lam D, Macomber J, Short JM, Robertson DE, Miller C (2002) A novel, high performance enzyme for starch liquefaction. J Biol Chem 277:26501–26507.

    Article  CAS  PubMed  Google Scholar 

  • Riesenfeld CS, Goodman RM, Handelsman J (2004a) Uncultured soil bacteria are a reservoir of new antibiotic resistance genes. Environ Microbiol 6:981–989.

    Article  CAS  PubMed  Google Scholar 

  • Riesenfeld CS, Schloss PD, Handelsman J (2004b) Metagenomics: Genomic analysis of microbial communities. Annu Rev Genet 38:525–552.

    Article  CAS  PubMed  Google Scholar 

  • Robe P, Nalin R, Capellano C, Vogel TM, Simonet P (2003) Extraction of DNA from soil. Eur J Soil Biol 39:183–190.

    Article  CAS  Google Scholar 

  • Robertson DE, Chaplin JA, DeSantis G, Podar M, Madden M, Chi E, Richardson T, Milan A, Miller M, Weiner DP, Wong K, McQuaid J, Farwell J, Preston LA, Tan X, Snead MA, Keller M, Mathur E, Kretz PL, Burk MJ, Short JM (2004) Exploring nitrilase sequence space for enantioselective catalysis. Appl Environ Microbiol 70:2429–2436.

    Article  CAS  PubMed  Google Scholar 

  • Ronaghi M, Uhlen M, Nylen P (1998) A sequencing method based on real-time pyrophosphate. Science 281:363–365.

    Article  CAS  PubMed  Google Scholar 

  • Rondon MR, August PR, Bettermann AD, Brady SF, Grossman TH, Liles MR, Loiacono KA, Lynch BA, MacNeil IA, Minor C, Tiong CL, Gilman M, Osburne MS, Clardy J, Handelsman J, Goodman RM (2000) Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl Environ Microbiol 66:2541–2547.

    Article  CAS  PubMed  Google Scholar 

  • Schleper C, Swanson RV, Mathur EJ, DeLong EF (1997) Characterization of a DNA polymerase from the uncultivated psychrophilic archaeon Cenarchaeum symbiosum. J Bacteriol 179:7803–7811.

    CAS  PubMed  Google Scholar 

  • Schloss PD, Handelsman J (2003) Biotechnological prospects from metagenomics. Curr Opin Biotechnol 14:303–310.

    Article  CAS  PubMed  Google Scholar 

  • Short JM (1997) Recombinant approaches for accessing biodiversity. Nat Biotechnol 15:1322–1323.

    Article  CAS  PubMed  Google Scholar 

  • Siddiqui KS, Cavicchioli R (2006) Cold-adapted enzymes. Annu Rev Biochem 75:403–433.

    Article  CAS  PubMed  Google Scholar 

  • Sjöling S, Stafford W, Cowan DA (2006) Soil metagenomics: Exploring and exploiting the soil microbial gene pool. In: van Elsas JD, Jansson J, Trevors JT (eds) Modern soil microbiology, 2nd edition. CRC Press, USA, pp 409–434.

    Google Scholar 

  • Sogin ML, Morrison HG, Huber JA, Welch DM, Huse SM, Neal PR, Arrieta JM, Herndl GJ (2006) Microbial diversity in the deep sea and the underexplored “rare biosphere”. Proc Natl Acad Sci USA 103:12115–12120.

    Article  CAS  PubMed  Google Scholar 

  • Song JS, Jeon JH, Lee JH, Jeong SH, Jeong BC, Kim SJ, Lee SH (2005) Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea). J Microbiol 43:172–178.

    CAS  PubMed  Google Scholar 

  • Stein JL, Marsh TL, Wu KY, Shizuya H, DeLong EF (1996) Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine Archaeon. J Bacteriol 178:591–599.

    CAS  PubMed  Google Scholar 

  • Strous M, Pelletier E, Mangenot S, Rattel T, Lehner A, Taylor MW, Horn M, Daims H, Bartol-Mavel D, Wickner P, Barbe V, Fonknechten N, Vallenet D, Segurens B, Schenowitz-Truong C, Médigue C, Collingro A, Snel B, Dutilh BE, Op den Camp JM, van der Drift C, Cirpus I, van de Pas-Schoonen KT, Harhangi HR, Niftrik L, Schmid M, Keltjens J, van de Vossenberg J, Kartal B, Meier H, Frishman D, Huynen MA, Mewes H-W, Weissenbach J, Jetten M, Wagner M, Le Paslier D (2006) Deciphering the evolution and metabolism of an anammox bacterium from a community genome. Nature 440:790–794.

    Article  PubMed  Google Scholar 

  • Teeling H, Waldmann J, Lombardot T, Bauer M, Glockner FO (2004) TETRA: a web-service and a stand-alone program for the analysis and comparison of tetranucleotide usage patterns in DNA sequences. BMC Bioinformatics 5.

    Google Scholar 

  • Tringe SG, von Mering C, Kobayashi A, Salamov AA, Chen K, Chang HW, Podar M, Short JM, Mathur EJ, Detter JC, Bork P, Hugenholtz P, Rubin EM (2005) Comparative Metagenomics of Microbial Communities. Science 308:554–557.

    Article  CAS  PubMed  Google Scholar 

  • Tyson GW, Chapman J, Hugenholtz P, Allen EE, Ram RJ, Richadson PM, Solovyev VV, Rubin EM, Rokhsar DS, Banfield JF (2004) Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature 428:37–43.

    Article  CAS  PubMed  Google Scholar 

  • Uchiyama T, Abe T, Ikemura T, Watanabe K (2005) Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes. Nat Biotechnol 23:88–93.

    Article  CAS  PubMed  Google Scholar 

  • Urbach E, Vergin KL, Giovannoni SJ (1999) Immunochemical detection and isolation of DNA from metabolically active bacteria. Appl Environ Microbiol 65:1207–1213.

    CAS  PubMed  Google Scholar 

  • Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KE, Nelson W, Fouts DE, Levy S, Knap AH, Lomas MW, Nealson K, White O, Peterson J, Hoffman J, Parsons R, Baden-Tillson H, Pfannkoch C, Rogers Y-H, Smith HO (2004) Environmental genome shotgun sequencing of the Sargasso Sea. Science 304:66–74.

    Article  CAS  PubMed  Google Scholar 

  • Voget S, Leggewie C, Uesbeck A, Raasch C, Jaeger KE, Streit WR (2003) Prospecting for novel biocatalysts in a soil metagenome. Appl Environ Microbiol 69:6235–6242.

    Article  CAS  PubMed  Google Scholar 

  • Wellington EMH, Berry A, Krsek M (2003) Resolving funtional diversity in relation to microbial community structure in soil: exploiting genomics and stable isotope probing. Curr Opin Microbiol 6:295–301.

    Article  CAS  PubMed  Google Scholar 

  • Whitaker RJ, Banfield JF (2006) Population genomics in natural microbial communities. Trends Ecol Evol 21:508–516.

    Article  PubMed  Google Scholar 

  • Whitley AS, Manefield M, Turner SL, Bailey MJ (2005) Lessons from the genomes: microbial ecology and genomics. In: Osburne MS, Smith CJ (eds) Molecular microbial ecology. Taylor and Francis Group, NY, pp 241–259.

    Google Scholar 

  • Wilson MS, Bakermans C, Madsen EL (1999) In situ, real-time catabolic gene expression: Extraction and characterization of naphthalene dioxygenase mRNA transcripts from groundwater. Appl Environ Microbiol 65:80–87.

    CAS  PubMed  Google Scholar 

  • Woyke T, Teeling H, Ivanova NN, Huntemann M, Richter M, Gloeckner FO, Boffelli D, Anderson IJ, Barry KW, Shapiro HJ, Szeto E, Kyrpides NC, Mussmann M, Amann R, Bergin C, Ruehland C, Rubin EM, Dubilier N (2006) Symbiosis insights through metagenomic analysis of a microbial consortium. Nature 443:950–955.

    Article  CAS  PubMed  Google Scholar 

  • Yokouchi H, Fukuoka Y, Mukoyama D, Calugay R, Takeyama H, Matsunaga T (2006) Whole-metagenome amplification of a microbial community associated with scleractinian coral by multiple displacement amplification using phi 29 polymerase. Environ Microbiol 8:1155–1163.

    Article  CAS  PubMed  Google Scholar 

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Sjöling, S., Cowan, D.A. (2008). Metagenomics: Microbial Community Genomes Revealed. In: Margesin, R., Schinner, F., Marx, JC., Gerday, C. (eds) Psychrophiles: from Biodiversity to Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74335-4_18

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