Abstract
An image analysis system (IAS) was developed for the quantitative assessment of estrogen receptor’s (ER) positive status from breast tissue microscopy images. Twenty-four cases of breast cancer biopsies, immunohistochemically (IHC) stained for ER, were microscopically assessed by a histopathologist, following a clinical routine scoring protocol. Digitized microscopy views of the specimens were used in the IAS’s design. IAS comprised a/image segmentation, for nuclei determination, b/extraction of textural features, by processing of nuclei-images utilizing the Laws and Gabor filters and by calculating textural features from the processed nuclei-images, and c/PNN and SVM classifiers design, for discriminating positively stained nuclei. The proportion of the latter in each case’s images was compared against the physician’s score. Using Spearman’s rank correlation, high correlation was found between the histopathogist’s and IAS’s scores (rho=0.89, p<0.001) and 22/24 cases were correctly characterised, indicating IAS’s reliability in the quantitative evaluation of ER as additional assistance to physician’s assessment.
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References
Sommer, S., Fuqua, S.A.: Estrogen receptor and breast cancer. Semin. Cancer Biol. 11, 339–352 (2001)
Donegan, W.L.: Tumor-related prognostic factors for breast cancer. CA Cancer J. Clin. 47, 28–51 (1997)
Jasani, B., Douglas-Jones, A., Rhodes, A., Wozniak, S., Barrett-Lee, P.J., Gee, J., Nicholson, R.: Measurement of estrogen receptor status by immunocytochemistry in paraffin wax sections. Methods Mol. Med. 120, 127–146 (2006)
Harvey, J.M., Clark, G.M., Osborne, C.K., Allred, D.C.: Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer. J. Clin. Oncol. 17, 1474–1481 (1999)
Diaz, L.K., Sneige, N.: Estrogen receptor analysis for breast cancer: Current issues and keys to increasing testing accuracy. Adv. Anat. Pathol. 12, 10–19 (2005)
Diaz, L.K., Sahin, A., Sneige, N.: Interobserver agreement for estrogen receptor immunohistochemical analysis in breast cancer: A comparison of manual and computer-assisted scoring methods. Ann. Diagn. Pathol. 8, 23–27 (2004)
Mofidi, R., Walsh, R., Ridgway, P.F., Crotty, T., McDermott, E.W., Keaveny, T.V., Duffy, M.J., Hill, A.D., O’Higgins, N.: Objective measurement of breast cancer oestrogen receptor status through digital image analysis. Eur. J. Surg. Oncol. 29, 20–24 (2003)
Lehr, H.A., Mankoff, D.A., Corwin, D., Santeusanio, G., Gown, A.M.: Application of photoshop-based image analysis to quantification of hormone receptor expression in breast cancer. J. Histochem. Cytochem. 45, 1559–1565 (1997)
Makkink-Nombrado, S.V., Baak, J.P., Schuurmans, L., Theeuwes, J.W., van der Aa, T.: Quantitative immunohistochemistry using the cas 200/486 image analysis system in invasive breast carcinoma: A reproducibility study. Anal. Cell Pathol. 8, 227–245 (1995)
Kostopoulos, S., Cavouras, D., Daskalakis, A., Ravazoula, P., Nikiforidis, G.: Image analysis system for assessing the estrogen receptor’s positive status in breast tissue carcinomas. In: Proceedings of the International Special Topic Conference on Information Technology in Biomedicine, Ioannina Greece (2006)
Schnorrenberg, F., Tsapatsoulis, N., Pattichis, C.S., Schizas, C.N., Kollias, S., Vassiliou, M., Adamou, A., Kyriacou, K.: Improved detection of breast cancer nuclei using modular neural networks. IEEE Eng. Med. Biol. Mag. 19, 48–63 (2000)
Patricio, M.A., Maravall, D.: A comparative study of contextual segmentation methods for digital angiogram analysis. Cybernetics and Systems: An International Journal 35, 63–83 (2004)
Laws, K.: Rapid texture identification. In: Proceedings of the Image Processing for Missile Guidance, pp. 376-380 (1980)
Theodoridis, S., Koutroumbas, K.: Pattern recognition, 2nd edn. Elsevier, San Diego (2003)
Haralick, R.M., Shanmugam, K., Dinstein, I.H.: Textural features for image classification. IEEE Trans. Sys. Man Cyb. 3, 610–621 (1973)
Galloway, M.M.: Texture analysis using gray-level run lengths. Computer Graphics and Image Processing 4, 172–179 (1975)
Grigorescu, S.E., Petkov, N., Kruizinga, P.: Comparison of texture features based on gabor filters. IEEE Trans. Image Processing 11 (2002)
Specht, D.F.: Probabilistic neural networks. Neural Networks 3, 109–118 (1990)
Christanini, N., Taylor, J.S.: An introduction to support vector machines and other kernel-based learning methods. Cambridge University Press, Cambridge (2000)
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Kostopoulos, S. et al. (2007). Assessing Estrogen Receptors’ Status by Texture Analysis of Breast Tissue Specimens and Pattern Recognition Methods. In: Kropatsch, W.G., Kampel, M., Hanbury, A. (eds) Computer Analysis of Images and Patterns. CAIP 2007. Lecture Notes in Computer Science, vol 4673. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74272-2_28
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DOI: https://doi.org/10.1007/978-3-540-74272-2_28
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