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Conceptual Models

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Cis/Transgene Optimization

Part of the book series: SpringerBriefs in Systems Biology ((BRIEFSBIOSYS))

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Abstract

In reviewing the literature, sometimes no or very little data are found on the specific expression elements for genetic engineering studies. This study set out with the aim of systematic discovery of the key upstream factors for genetic manipulation of microalgae as a case study. The review indicates how the MARs flanking the expression cassette along with the optimized expression elements could potentially improve the transformation efficiency and stability. The outlines can be efficiently deployed as a practical model for systematic discovery of the key expression elements and optimization of the cis/transgenes in other micro/organisms in which there is limited information about these factors.

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Authors and Affiliations

  1. Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran

    Saeid Kadkhodaei

  2. Plant Genetics, AgroBioChem Department, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium

    Farahnaz Sadat Golestan Hashemi

  3. Tropical Infectious Diseases Research and Education Centre (TIDREC), Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

    Morvarid Akhavan Rezaei

  4. Halal Products Research Institute, Universiti Putra Malaysia, Seri Kembangan, Malaysia

    Sahar Abbasiliasi

  5. Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, George Town, Penang, Malaysia

    Joo Shun Tan

  6. SynHiTech, Thornhill, ON, Canada

    Hamid Rajabi Memari

  7. Department of Veterinary Services, Ministry of Animal Health and Fisheries Development, Usman Faruk Secretariat, Sokoto, Sokoto, Nigeria

    Faruku Bande

  8. Mater Research, Translational Research Institute, Woolloongabba, QLD, Australia

    Ali Baradaran

  9. Faculty of Medicine, Translational Research Institute, Diamantina Institute, University of Queensland, Brisbane, QLD, Australia

    Ali Baradaran

  10. Institute of plantation studies, Universiti Putra Malaysia, Seri Kembangan, Malaysia

    Mahdi Moradpour

  11. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Seri Kembangan, Malaysia

    Arbakariya B. Ariff

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  1. Saeid Kadkhodaei
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  2. Farahnaz Sadat Golestan Hashemi
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Correspondence to Saeid Kadkhodaei .

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Kadkhodaei, S. et al. (2018). Conceptual Models. In: Cis/Transgene Optimization. SpringerBriefs in Systems Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-90391-0_4

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