Abstract
Somatic embryogenesis (SE) offers a basis for scalable, automated technology suitable for large-scale production of clonal plants. The SE method is attractive biologically due to the developmental path of the somatic embryo closely resembling zygotic embryo development thus avoiding issues related to adventitious rooting and plagiotropic growth. Furthermore, long term storage of SE cultures allow for field testing in species where zygotic seeds are the starting material for SE cultures, e.g. conifers. Application of SE methods for industrial scale plant production has been limited due to the cost of labor involved with different steps of the SE process.
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References
Aidun CK (2013) Fluidics-based orientation and sorting device for plant embryos. Assignee—Georgia Tech Research Corporation. Patent 8452460. Issued 28 May 2013
Aidun CK (2015a) Methods for dispersing somatic plant embryos. Assignee—Georgia Tech Research Corporation. Patent 8452460. Issued 10 Mar 2015
Aidun CK (2015b) Separator device, depostion device, and system for handling of somatic embryos. Assignee—Georgia Tech Research Corporation. Patent 9040301. Issued 26 May 2015
Aidun CK (2015c) Planting method and device for plant propagules. Applicant—Georgia Tech Research Corporation. Patent publication no. WO/2015/097603. Published 2 July 2015
Aidun CK (2016) Integrated germination method and device. Applicant—Georgia Tech Research Corporation. Patent publication no. WO2016098083 A1. Published 23 June 2016
Aidun CK, Egertsdotter U (2012) Fluidics-based automation of clonal propagation via somatic embryogenesis: SE-fluidics system. In Proceedings of 2nd IUFRO working party, Bruno, Czech Republic, 2.09.02 25-8, June 2012
Aidun CK, Egertsdotter U (2015) Method for dispersion of assemblies of biological material. Assignee—Georgia Tech Research Corporation, Patent 8927287. Issued 6 Jan 2015
Aronen T, Egertsdotter U (2014) Close to application of somatic embryogenesis. SNS News Views 6:2014
Businge E, Brackmann K, Moritz T, Egertsdotter U (2012) Metabolite profiling reveals clear metabolic changes during somatic embryo development of Norway spruce (Picea abies). Tree Physiol 32(2):232–244
Businge E, Bygdell J, Wingsle G, Moritz T, Egertsdotter U (2013) The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos. Physiol Plant 149(2):273–285
Businge E, Trifonova A, Schneider C, Rödel P, Egertsdotter U (2017) Evaluation of a new temporary immersion bioreactor system for micropropagation of cultivars of eucalyptus, birch and fir. Forests 8(6):196
Dobrowolska I, Businge E, Abreu IN, Moritz T, Egertsdotter U (2017) Metabolome and transcriptome profiling reveal new insights into somatic embryo germination in Norway spruce (Picea abies). Tree Physiol 00:1–15. https://doi.org/10.1093/treephys/tpx078
Filonova LH, Bozhkov PV, von Arnold S (2000) Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. J Exp Bot 51:249–264
Högberg K-A, Ekberg I, Norell L, von Arnold S (1998) Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies. Can J Forest Res 28:1536–1545
Kong L, von Aderkas P (2011) A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer. Plant Cell, Tissue Organ Cult 106:115–125
Lelu-Walter M-A, Thompson D, Harvengt L, Sanchez L, Toribio M, Pâgues LE (2013) Somatic embryogenesis in forestry with a focus on Europe: state-of-the-art, benefits, challenges and future. Tree Genet. Genomes 9:883–899
Lu G, Fei B (2014) Medical hyperspectral imaging: a review. J Biomed Opt 19(1):010901
Mamun NHA, Egertsdotter U, Aidun CK (2014) Bioreactor technology for clonal propagation of plants and metabolite production. Front Biol 10(2):177–193
Sharma S, Shahzad A, Teixeira da Silva JA (2013) Synseed technology—a complete synthesis. Biotech Adv 31:186–207
Swedish Statistical Yearbook of Forestry (2014) Skogsstyrelsen, Swedish Forest Agency, ISSN 0491-7847, ISBN 978-91-87535-05-5
Acknowledgements
Several companies and research foundations have funded various parts of the development of the automation system. We acknowledge financial support from SweTree Technologies and it’s consortium of associated Swedish forest companies, VINNOVA Swedish innovation agency and the Kempe Foundations. We also acknowledge the support and hard work of all engineers and scientists involved with these projects.
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Aidun, C.K., Egertsdotter, U. (2018). SE Fluidics System. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_16
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DOI: https://doi.org/10.1007/978-3-319-89483-6_16
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