Skip to main content
SpringerLink
Log in

Supercritical Fluid Technology as a Tool to Prepare Gradient Multifunctional Architectures Towards Regeneration of Osteochondral Injuries

  • Chapter
  • First Online:
Osteochondral Tissue Engineering

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1058))

Abstract

Platelet lysates (PLs) are a natural source of growth factors (GFs) known for its stimulatory role on stem cells which can be obtained after activation of platelets from blood plasma. The possibility to use PLs as growth factor source for tissue healing and regeneration has been pursued following different strategies. Platelet lysates are an enriched pool of growth factors which can be used as either a GFs source or as a three-dimensional (3D) hydrogel. However, most of current PLs-based hydrogels lack stability, exhibiting significant shrinking behavior. This chapter focuses on the application of supercritical fluid technology to develop three-dimensional architectures of PL constructs, crosslinked with genipin. The proposed technology allows in a single step operation the development of mechanically stable porous structures, through chemical crosslinking of the growth factors present in the PL pool, followed by supercritical drying of the samples. Furthermore gradient structures of PL-based structures with bioactive glass are also presented and are described as an interesting approach to the treatment of osteochondral defects.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Yang PJ, Temenoff JS (2009) Engineering orthopedic tissue interfaces. Tissue Eng. Part B Rev. 15:127–141

    Article  CAS  Google Scholar 

  2. Ahmed TAE, Hincke MT (2010) Strategies for articular cartilage lesion repair and functional restoration. Tissue Eng. Part B. Rev. 16:305–329

    Article  CAS  Google Scholar 

  3. Lefebvre V, Smits P (2005) Transcriptional control of chondrocyte fate and differentiation. Birth Defects Res Part C Embryo Today Rev 75:200–212

    Article  CAS  Google Scholar 

  4. Malafaya PB, Silva GA, Reis RL (2007) Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Adv Drug Deliv Rev 59:207–233

    Article  CAS  Google Scholar 

  5. O’Shea TM, Miao X (2008) Bilayered scaffolds for osteochondral tissue engineering. Tissue Eng. Part B. Rev. 14:447–464

    Article  Google Scholar 

  6. Chen J et al (2011) Simultaneous regeneration of articular cartilage and subchondral bone in vivo using MSCs induced by a spatially controlled gene delivery system in bilayered integrated scaffolds. Biomaterials 32:4793–4805

    Article  CAS  Google Scholar 

  7. Mano JF, Reis RL (2007) Osteochondral defects: present situation and tissue engineering approaches. J. Tissue Eng. Regen. Med. 1:261–273

    Article  CAS  Google Scholar 

  8. Chen FM, Zhang M, Wu ZF (2010) Toward delivery of multiple growth factors in tissue engineering. Biomaterials 31:6279–6308

    Article  CAS  Google Scholar 

  9. Kon E, Mutini A, Arcangeli E, Delcogliano M, Filardo G, Nicoli Aldini N, Pressato D, Quarto R, Zaffagnini S, Marcacci M (2008) Novel nanostructured scaffold for osteochondral regeneration: pilot study in horses. J. Tissue Eng. Regen. Med. 2:408–417

    Article  Google Scholar 

  10. Lu HH, Subramony SD, Boushell MK, Zhang X (2010) Tissue engineering strategies for the regeneration of orthopedic interfaces. Ann. Biomed. Eng. 38:2142–2154

    Article  Google Scholar 

  11. Anitua E et al (2006) New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 24:227–234

    Article  CAS  Google Scholar 

  12. Haberhauer M et al (2008) Cartilage tissue engineering in plasma and whole blood scaffolds. Adv. Mater. 20:2061–2067

    Article  CAS  Google Scholar 

  13. Santo VE et al (2012) Enhancement of osteogenic differentiation of human adipose derived stem cells by the controlled release of platelet lysates from hybrid scaffolds produced by supercritical fluid foaming. J. Control. Release 162:19–27

    Article  CAS  Google Scholar 

  14. Duarte a RC, Mano JF, Reis RL (2009) Perspectives on: supercritical fluid technology for 3D tissue engineering scaffold applications. J. Bioact. Compat. Polym. 24:385–400

    Article  CAS  Google Scholar 

  15. Duarte ARC et al (2013) Unleashing the potential of supercritical fluids for polymer processing in tissue engineering and regenerative medicine. J. Supercrit. Fluids 79:177–185

    Article  CAS  Google Scholar 

  16. Santo VE, Duarte ARC, Gomes ME, Mano JF, Reis RL (2010) Hybrid 3D structure of poly(d,l-lactic acid) loaded with chitosan/chondroitin sulfate nanoparticles to be used as carriers for biomacromolecules in tissue engineering. J Supercrit Fluids 54:320–327

    Article  CAS  Google Scholar 

  17. van de Witte P, Dijkstra PJJ, van den Berg JWA, Feijen J (1996) Phase separation processes in polymer solutions in relation to membrane formation. J. Memb. Sci. 117:1–31

    Article  Google Scholar 

  18. Eckert CA, Knutson BL, Debenedetti PG (1996) Supercritical fluids as solvents for chemical and materials processing. Nature 383:313–318

    Article  CAS  Google Scholar 

  19. Temtem M et al (2009) Supercritical CO2 generating chitosan devices with controlled morphology. Potential application for drug delivery and mesenchymal stem cell culture. J. Supercrit. Fluids 48:269–277

    Article  CAS  Google Scholar 

  20. Keeney M, Pandit A (2009) The osteochondral junction and its repair via bi-phasic tissue engineering scaffolds. Tissue Eng. Part B. Rev. 15:55–73

    Article  CAS  Google Scholar 

  21. Gadjanski I, Vunjak-Novakovic G (2015) Challenges in engineering osteochondral tissue grafts with hierarchical structures. Expert Opin. Biol. Ther. 2598:1–17

    Google Scholar 

  22. Nukavarapu SP, Dorcemus DL (2012) Osteochondral tissue engineering: current strategies and challenges. Biotechnol Adv. https://doi.org/10.1016/j.biotechadv.2012.11.004

  23. Canadas RF, Marques AP, Reis RL, Oliveira JM (2017) In: Oliveira JM, Reis RL (eds) Regenerative strategies for the treatment of knee joint disabilities. Springer International, Berlin, pp 213–233. https://doi.org/10.1007/978-3-319-44785-8_11

    Chapter  Google Scholar 

  24. Yan LP et al (2015) Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance. Acta Biomater. 12:227–241

    Article  CAS  Google Scholar 

  25. Yan LP, Oliveira JM, Oliveira AL, Reis RL (2013) Silk fibroin/nano-CaP bilayered scaffolds for osteochondral tissue engineering. Key Eng. Mater. 587:245–248

    Article  Google Scholar 

  26. Zaky SH, Ottonello A, Strada P, Cancedda R, Mastrogiacomo M (2008) Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering. J. Tissue Eng. Regen. Med. 2:472–481

    Article  CAS  Google Scholar 

  27. Santo VE et al (2016) Engineering enriched microenvironments with gradients of platelet lysate in hydrogel fibers. Biomacromolecules 17:1985–1997

    Article  CAS  Google Scholar 

  28. Babo PS et al (2016) Assessment of bone healing ability of calcium phosphate cements loaded with platelet lysate in rat calvarial defects. J. Biomater. Appl. 31:637–649

    Article  CAS  Google Scholar 

  29. Yan L, Oliveira JM, Oliveira AL, Reis RL (2015) Current concepts and challenges in osteochondral tissue engineering and regenerative medicine. ACS Biomater. Sci. Eng. 1(4):150220124046001. https://doi.org/10.1021/ab500038y

    Article  CAS  Google Scholar 

  30. Ribeiro V, Pina S, Oliveira JM, Reis RL (2017) In: Oliveira JM, Reis RL (eds) Regenerative strategies for the treatment of knee joint disabilities. Springer International, Berlin, pp 129–146. https://doi.org/10.1007/978-3-319-44785-8_7

    Chapter  Google Scholar 

  31. Cengiz IF, Oliveira JM, Reis RL (2014) In: Magnenat-Thalmann N, Ratib O, Choi HF (eds) 3D multiscale physiological human. Springer, London, pp 25–47. https://doi.org/10.1007/978-1-4471-6275-9_2

    Chapter  Google Scholar 

  32. Wang C, Lau TT, Loh WL, Su K, Wang D (2011) Cytocompatibility study of a natural biomaterial crosslinker—Genipin with therapeutic model cells. J Biomed Mater Res B Appl Biomater 97:58–65. https://doi.org/10.1002/jbm.b.31786

    Article  CAS  PubMed  Google Scholar 

  33. Muzzarelli RAA (2009) Genipin-crosslinked chitosan hydrogels as biomedical and pharmaceutical aids. Carbohydr. Polym. 77:1–9

    Article  CAS  Google Scholar 

  34. Butler MF, Ng Y, Pudney PDA (2003) Mechanism and kinetics of the crosslinking reaction between biopolymers containing primary amine groups and Genipin. J Polym Sci Part A Polym Chem 41:3941–3953

    Article  CAS  Google Scholar 

  35. Mu C, Zhang K, Lin W, Li D (2012) Ring-opening polymerization of genipin and its long-range crosslinking effect on collagen hydrogel. J Biomed Mater Res A 101:385–393. https://doi.org/10.1002/jbm.a.34338

    Article  CAS  PubMed  Google Scholar 

  36. Chuang M, Johannsen M (2009) Characterization of pH in aqueous CO2—systems. Polym Degrad Stab 97(6):839–848

    Article  Google Scholar 

  37. Babo P et al (2014) Platelet lysate membranes as new autologous templates for tissue engineering applications. Inflamm. Regen. 34:033–044

    Article  CAS  Google Scholar 

  38. Santo VE, Popa EG, Mano JF, Gomes ME, Reis RL (2015) Natural assembly of platelet lysate-loaded nanocarriers into enriched 3D hydrogels for cartilage regeneration. Acta Biomater. 19:56–65

    Article  CAS  Google Scholar 

  39. Duarte ARC, Caridade SG, Mano JF, Reis RL (2009) Processing of novel bioactive polymeric matrixes for tissue engineering using supercritical fluid technology. Mater. Sci. Eng. C 29:2110–2115

    Article  CAS  Google Scholar 

  40. Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR (2006) Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 27:3413–3431

    Article  CAS  Google Scholar 

  41. Rey C, Combes C (2016) Biomineralization and biomaterials. pp 95–127. https://doi.org/10.1016/B978-1-78242-338-6.00004-1

    Chapter  Google Scholar 

Download references

Acknowledgements

The research leading to these results has received funding from the project “Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells,” supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).

Author information

Authors and Affiliations

  1. 3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Barco/Guimarães, Portugal

    Ana Rita C. Duarte, Vitor E. Santo, Manuela E. Gomes & Rui L. Reis

  2. ICVS/3B’s—PT Government Associate Laboratory, Braga/Guimarães, Portugal

    Ana Rita C. Duarte, Vitor E. Santo, Manuela E. Gomes & Rui L. Reis

Authors
  1. Ana Rita C. Duarte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vitor E. Santo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Manuela E. Gomes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rui L. Reis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ana Rita C. Duarte .

Editor information

Editors and Affiliations

  1. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, Guimarães, Portugal

    J. Miguel Oliveira

  2. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, Guimarães, Portugal

    Sandra Pina

  3. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, Guimarães, Portugal

    Rui L. Reis

  4. Polymeric Nanomaterials and Biomaterials Department, Institute of Polymer Science and Technology, Spanish Council for Scientific Research (CSIC), Madrid, Spain

    Julio San Roman

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Duarte, A.R.C., Santo, V.E., Gomes, M.E., Reis, R.L. (2018). Supercritical Fluid Technology as a Tool to Prepare Gradient Multifunctional Architectures Towards Regeneration of Osteochondral Injuries. In: Oliveira, J., Pina, S., Reis, R., San Roman, J. (eds) Osteochondral Tissue Engineering. Advances in Experimental Medicine and Biology, vol 1058. Springer, Cham. https://doi.org/10.1007/978-3-319-76711-6_12

Download citation

Publish with us

Policies and ethics

Search

Navigation