Abstract
An ability to identify SSCs by their transcriptome analysis would greatly benefit future treatment of male infertility. If you could determine the RNA expression of every stage of spermatogenesis, that would be a more reliable identification of cell type by gene expression than just histology. It would mean the elucidation of the total genetics of spermatogenesis. The identification of spermatogonial stem cells (SSCs) in particular in the human would help us better understand the origin of testicular cancers such as teratoma, choriocarcinoma, embryonal carcinoma, and seminoma, which arise in the testis from germ cells [1–5]. It is known that primordial germ cells (PGCs) arise from the fetal epiblast at the time of gastrulation and migrate eventually to the gonadal ridge to become spermatogonial stem cells (SSCs) in the male gonad. Any pluripotent stem cells that have not fully differentiated into PGCs when they enter the fetal testis could become a testis cancer. In fact cancers of the adult testis are composed of differentiated cells which represent all basic somatic cell types, much like what ES cells develop into if not suppressed in culture or in a niche. So the origin of testis cancer is most likely embryonic stem cells rests that entered the testis before fully converting to PGCs.
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References
McLachlan RI, Rajpert-De Meyts E, Hoei-Hansen CE, de Kretser DM, Skakkebaek NE (2007) Histological evaluation of the human testis-approaches to optimizing the clinical value of the assessment: mini review. Hum Reprod 22:2–16
Dohle GR, Elzanaty S, van Casteren NJ (2012) Testicular biopsy: clinical practice and interpretation. Asian J Androl 14:88–93
Abdullah L, Bondagji N (2011) Histopathological patterns of testicular biopsy in male infertility: a retrospective study from a tertiary care center in the western part of Saudi Arabia. Urol Ann 3:19–23
Silber SJ (2000) Microsurgical TESE and the distribution of spermatogenesis in non-obstructive azoospermia. Hum Reprod 15:2278–2284
Silber SJ, van Steirteghem A, Nagy Z, Liu J, Tournaye H, Devroey P (1996) Normal pregnancies resulting from testicular sperm extraction and intracytoplasmic sperm injection for azoospermia due to maturation arrest. Fertil Steril 66:110–117
Silber SJ, Nagy Z, Devroey P, Tournaye H, Van Steirteghem AC (1997) Distribution of spermatogenesis in the testicles of azoospermic men: the presence or absence of spermatids in the testes of men with germinal failure. Hum Reprod 12:2422–2428
Fossa SD, Magelssen H (2004) Fertility and reproduction after chemotherapy of adult cancer patients: malignant lymphoma and testicular cancer. Ann Oncol 15(Suppl 4):iv259–iv265
Howell SJ, Shalet SM (2005) Spermatogenesis after cancer treatment: damage and recovery. J Natl Cancer Inst Monogr (34):12–17
Further Reading
Barrios F, Irie N, Surani MA (2013) Perceiving signals, building networks, reprogramming germ cell fate. Int J Dev Biol 57:123–132
van den Berg H, Repping S, van der Veen F (2007) Parental desire and acceptability of spermatogonial stem cell cryopreservation in boys with cancer. Hum Reprod 22(2):594–597
Brinster RL (2007) Male germline stem cells: from mice to men. Science 316(5823):404–405
Brinster RL, Avarbock MR (1994) Germline transmission of donor haplotype following spermatogonial transplantation. Proc Natl Acad Sci U S A 91:11303–11307
Brinster RL, Zimmerman JW (1994) Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci U S A 91:11289–11302
Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M (2011) Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. Cell 146:519–532
Ishikura Y, Yabuta Y, Ohta H et al (2016) In vitro derivation and propagation of spermatogonial stem cell activity from mouse pluripotent stem cells. Cell Rep 17:2789–2804
Jeruss JS, Woodruff TK (2009) Preservation of fertility in patients with cancer. N Engl J Med 360(9):902–911
Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, Shinohara T (2003) Long term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod 69(2):612–616
Nagano M, Avarbock MR, Leonida EB, Brinster CJ, Brinster RL (1998) Culture of mouse spermatogonial stem cells. Tissue Cell 30(4):389–397
Nagano M, Brinster RL (1998) Spermatogonial transplantation and reconstitution of donor cell spermatogenesis in recipient males. Acta Pathol Microbiol Immunol Scand 106:47–55
Nickkholgh B, Mizrak SC, van Daalen SK et al (2014) Genetic and epigenetic stability of human spermatogonial stem cells during long-term culture. Fertil Steril 102:1700–7 e1
Ogawa T, Arechnaga JM, Avarbock MR, Brinster RL (1997) Transplantation of testis germinal cells into mouse seminiferous tubules. Int J Dev Biol 41(1):111–122
Sadri-Ardekani H, Atala A (2014) Testicular tissue cryopreservation and spermatogonial stem cell transplantation to restore fertility: from bench to bedside. Stem Cell Res Ther 5:68
Saitou M, Miyauchi H (2016) Gametogenesis from pluripotent stem cells. Cell Stem Cell 18:721–735
Wallace WH, Anderson RA, Irvine DS (2005) Fertility preservation for young patients with cancer: who is at risk and what can be offered? Lancet Oncol. 6(4):209–218
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Silber, S. (2018). Identification of Spermatogonial Stem Cells and Genetic Control of All Stages of Spermatogenesis. In: Fundamentals of Male Infertility. Springer, Cham. https://doi.org/10.1007/978-3-319-76523-5_20
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