Abstract
The epiCS® skin irritation test (SIT) method is based on epiCS®, a reconstructed human epidermis, produced with normal human keratinocytes. During production, these cells differentiate in a multilayered epidermis that serves as a model for human in vivo epidermis. For the epiCS® SIT method, the tissues are exposed to test substances for 20 min followed by a 42 h post-incubation period. Subsequently, the relative viability of the tissues is determined by an MTT assay and is used to differentiate between irritants and non-irritants. A me-too validation study of the epiCS® SIT method was conducted involving EURL ECVAM and the independent scientific peer reviewing by the EURL ECVAM Scientific Advisory Committee. With the positive ESAC opinion, the epiCS® SIT method will be proposed for incorporation into the OECD Test Guideline 439.
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Engelking, O., Fuchs, H.W., Weisensee, D. (2017). The epiCS® Skin Irritation Test (SIT) Method. In: Eskes, C., van Vliet, E., Maibach, H. (eds) Alternatives for Dermal Toxicity Testing. Springer, Cham. https://doi.org/10.1007/978-3-319-50353-0_6
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