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Advancements of Mass Spectrometry in Biomedical Research pp 265–287Cite as

Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1140))

Abstract

Recent developments of mass spectrometry (MS) allow us to identify, estimate, and characterize proteins and protein complexes. At the same time, structural biology helps to determine the protein structure and its structure-function relationship. Together, they aid to understand the protein structure, property, function, protein-complex assembly, protein-protein interaction, and dynamics. The present chapter is organized with illustrative results to demonstrate how experimental mass spectrometry can be combined with computational structural biology for detailed studies of protein’s structures. We have used tumor differentiation factor protein/peptide as ligand and Hsp70/Hsp90 as receptor protein as examples to study ligand-protein interaction. To investigate possible protein conformation, we will describe two proteins—lysozyme and myoglobin. As an application of MS-based assignment of disulfide bridges, the case of the spider venom polypeptide Phα1β will also be discussed.

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Acknowledgments

KLW was supported by the ASPIRE Graduate Student Fellowship through Clarkson University’s CUPO Office. MM was supported by the Fulbright Senior Postdoctoral Fellowship awarded by the Romania-USA Fulbright Commission to MM (guest) and CCD (host). MM and CCD are thankful to the newly established Erasmus+ exchange program between “Al. I. Cuza” University of Iasi, Romania and Clarkson University of Potsdam, NY, USA.

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Authors and Affiliations

  1. Biochemistry & Proteomics Group, Structural Biology & Molecular Modeling Unit, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Marius Mihăşan, Kelly L. Wormwood, Izabela Sokolowska, Urmi Roy, Alisa G. Woods & Costel C. Darie

  2. Department of Biology, Alexandru Ioan Cuza University of Iasi, Iasi, Romania

    Marius Mihăşan

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  1. Marius Mihăşan
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  2. Kelly L. Wormwood
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Correspondence to Costel C. Darie .

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  1. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Alisa G. Woods

  2. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Costel C. Darie

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Mihăşan, M., Wormwood, K.L., Sokolowska, I., Roy, U., Woods, A.G., Darie, C.C. (2019). Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 1140. Springer, Cham. https://doi.org/10.1007/978-3-030-15950-4_15

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