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Methods for Exploring Soil Microbial Diversity

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Unravelling the Soil Microbiome

Part of the book series: SpringerBriefs in Environmental Science ((BRIEFSENVIRONMENTAL))

Abstract

Belowground microbial processes are at the helm of terrestrial ecosystem functions, and the enormous diversity of soil microorganisms acts as a key player. Thus, understanding the community dynamics of microorganisms in the soil is essential to know their distribution, abundance, and structure. Further, it is also important to know how these communities are shaped in structure and function in response to changes in space and time. Various microbial diversity analysis methods—fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphisms, and the automated version of ribosomal intergenic spacer analysis, RISA (ARISA)—have been developed to analyse the diversity of soil microorganisms based on their genetic structure. However, methods such as phospholipid and fatty acid analysis utilise the differences in lipid components of the microbial cell membrane to analyse their diversity. In the present chapter, we explore the conventional methods of soil microbial diversity analysis.

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References

  • Abdeljailil NO-B, Renault D, Gerbore J, Vallance J, Rey P, Daami-Remadi M (2016) Evaluation of the effectiveness of tomato-associated rhizobacter applied singly or as three-strain consortium for biosuppression of Sclerotinia stem rot in tomato. J Microb Biochem Technol 8:4

    Google Scholar 

  • Amann R, Fuchs BM (2008) Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques. Nat Rev Microbiol 6:339–348

    Article  CAS  Google Scholar 

  • Baraniya D, Puglisi E, Ceccherini MT, Pietramellara G, Giagnoni L, Arenella M, Renella G (2016) Protease encoding microbial communities and protease activity of the rhizosphere and bulk soils of two maize lines with different N uptake efficiency. Soil Biol Biochem 96:176–179

    Article  CAS  Google Scholar 

  • Becker JM, Parkin T, Nakatsu CH, Wilbur JD, Konopka A (2006) Bacterial activity, community structure and centimeter-scale spatial heterogeneity in contaminated soils. Microbiol Ecol 51:220–231

    Article  Google Scholar 

  • Ben-David EA, Holden PJ, Stone DJ, Harch BD, Foster LJ (2004) The use of phospholipid fatty acid analysis to measure impact of acid rock drainage on microbial communities in sediments. Microb Ecol 48:300–315

    Article  CAS  Google Scholar 

  • Ben-David EA, Zaady E, Sher Y, Nejidat A (2011) Assessment of the spatial distribution of soil microbial communities in patchy arid and semi-arid landscapes of the Negev Desert using combined PLFA and DGGE analyses. FEMS Microbiol Ecol 76:492–450

    Article  CAS  Google Scholar 

  • Bertani I, Abbruscato P, Piffanelli P, Subramoni S, Venturi V (2016) Rice bacterial endophytes: isolation of a collection, identification of beneficial strains and microbiome analysis. https://doi.org/10.1111/1758-2229.12403

    Article  CAS  Google Scholar 

  • Bodrossy L, Sessitsch A (2004) Oligonucleotide microarrays in microbial diagnostics. Curr Opin Microbiol 7:245–254

    Article  CAS  Google Scholar 

  • Brown MV, Fuhrman JA (2005) Marine bacterial microdiversity as revealed by internal transcribed spacer analysis. Aquat Microb Ecol 41:15–23

    Article  Google Scholar 

  • Butterly CR, Phillips LA, Wiltshire JL, Franksc AE, Armstronga RD, Chene D, Melea PM, Tanga C (2016) Long-term effects of elevated CO2 on carbon and nitrogen functional capacity of microbial communities in three contrasting soils. Soil Biol Biochem 97:157–167

    Article  CAS  Google Scholar 

  • Calderon FJ, Nielsen ID, Acosta-Martinez V, Vigil MF, Lyon D (2016) Cover crop and irrigation effects on soil microbial communities and enzymes in semiarid agroecosystems of the central Great Plains of North America. Pedosphere 26:192–205

    Article  Google Scholar 

  • Caracciolo AB, Bottoni P, Grenni P (2010) Fluorescence in situ hybridization in soil and water ecosystems: a useful method for studying the effect of xenobiotics on bacterial community structure. Toxicol Environ Chem 92:567–579

    Article  CAS  Google Scholar 

  • Chen Y, Murrell JC (2010) When metagenomics meets stable-isotope probing: progress and perspectives. Trends Microbiol 18:157–163

    Article  CAS  Google Scholar 

  • Chen Y, Dumont MG, McNamara NP, Chamberlain PM, Bodrossy L, Pavese NS, Murrell JC (2008) Diversity of the active methanotrophic community in acidic peatlands as assessed by mRNA and SIP-PLFA analyses. Environ Microbiol 110:446–459

    Article  CAS  Google Scholar 

  • Collins G, Kavanagh S, Mchugh S, Connaughton S, Kearney A, Rice O, Carrigg C, Scully C, Bhreathnach N, Mahony T, Madden P, Enright AM, Flaherty V (2006) Accessing the black box of microbial diversity and ecophysiology: recent advances through polyphasic experiments. J Environ Sci Health A 41:897–922

    Article  CAS  Google Scholar 

  • Costa R, Götz M, Mrotzek N, Lottmann J, Berg G, Smalla K (2006) Effects of site and plant species on rhizosphere community structure as revealed by molecular analysis of different microbial guilds. FEMS Microbiol Ecol 56:236–249

    Article  CAS  Google Scholar 

  • de la Luz Mora M, Demaneta R, Acunaa JJ, Viscardia S, Jorqueraa M, Rengelb Z, Duran P (2017) Aluminium-tolerant bacteria improve the plant growth and phosphorus content in ryegrass grown in a volcanic soil amended with cattle dung manure. Appl Soil Ecol 115:19–26

    Article  Google Scholar 

  • Degefu Y, Somervuo P, Aittamaa M, Virtanen E, Valkonen JPT (2016) Evaluation of a diagnostic microarray for the detection of major bacterial pathogens of potato from tuber samples. Bull OEPP 46:103–111

    Article  Google Scholar 

  • DeLong EF, Wickham GS, Pace NR (1989) Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science 243:1360–1363

    Article  CAS  Google Scholar 

  • Drenovsky RE, Steenwerth KL, Jackson LE, Scow KM (2010) Land use and climatic factors structure regional patterns in soil microbial communities. Glob Ecol Biogeogr 19:27–39

    Article  Google Scholar 

  • Drigo B, Van Veen JA, Kowalchuk GA (2009) Specific rhizosphere bacterial and fungal groups respond differently to elevated atmospheric CO2. ISME J 3:1204–1217

    Article  CAS  Google Scholar 

  • Dumont MG, Murrell JC (2005) Stable isotope probing-linking microbial identity to function. Nat Rev Microbiol 3:499–504

    Article  CAS  Google Scholar 

  • Fernandes JP, Marisa C, Almeida R, Andreotti F, Barros L, Almeida T, Mucha P (2017) Response of microbial communities colonizing salt marsh plants rhizosphere to copper oxide nanoparticles contamination and its implications for phytoremediation processes. Sci Total Environ 581:801–810

    Article  CAS  Google Scholar 

  • Fierer N, Jackson JA, Vilgalys R, Jackson RB (2005) Assessment of soil microbialcommunity structure by use of taxon-specific quantitative PCR assays. Appl Environ Microbiol 71:4117–4120

    Article  CAS  Google Scholar 

  • Fischer SG, Lerman LS (1979) Length-independent separation of DNA restriction fragments in two dimensional gel electrophoresis. Cell 16:191–200

    Article  CAS  Google Scholar 

  • Franzmann PD, Patterson BM, Power TR, Nichols PD, Davis GB (1996) Microbial biomass in a shallow, urban aquifer contaminated with aromatic hydrocarbons: analysis of phospholipid fatty acid content and composition. J Appl Bacteriol 80:617–625

    Article  CAS  Google Scholar 

  • Gebreil AS, Abraham W-R (2016) Diversity and activity of bacterial biofilm communities growing on hexachlorocyclohexane. Water Air Soil Pollut 227:295

    Article  CAS  Google Scholar 

  • Gruber-Dorninger C, Pester M, Kitzinger K, Savio DF, Loy A, Rattei T, Daims H (2015) Functionally relevant diversity of closely related Nitrospira in activated sludge. ISME J 9:643–655

    Article  CAS  Google Scholar 

  • Guangyin Z, Xueqin L, Takuro K, Gopalakrishnan K, Kaiqin X (2016) Promoted electromethanosynthesis in a two-chamber microbial electrolysis cells (MECs) containing a hybrid biocathode covered with graphite felt (GF). Chem Eng J 284:1146–1155.

    Google Scholar 

  • Hayashi K (1991) PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA. PCR Methods Appl 1:34–38

    Article  CAS  Google Scholar 

  • Hewson I, Fuhrman JA (2004) Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia. Appl Environ Microbiol 70:3425–3433

    Article  CAS  Google Scholar 

  • Higuchi R, Dollinger G, Walsh PS, Griffith R (1992) Simultaneous amplification and detection of specific DNA sequences. Nat Biotechnol 10:413–417

    Article  CAS  Google Scholar 

  • Huyghe A, Francois P, Schrenzel J (2009) Characterization of microbial pathogens by DNA microarrays. Infect Genet Evol 9:987–995

    Article  CAS  Google Scholar 

  • Ishii K, MuĂźmann M, MacGregor BJ, Amann R (2004) An improved fluorescence in situ hybridization protocol for the identification of bacteria and archaea in marine sediments. FEMS Microbiol Ecol 50:203–212

    Article  CAS  Google Scholar 

  • Janczyk P, Pieper R, Smidt H, Wolfgang B (2010) Souffrant effect of alginate and inulin on intestinal microbial ecology of weanling pigs reared under different husbandry conditions. FEMS Microbiol Ecol 72:132–142

    Article  CAS  Google Scholar 

  • Jousset A, Lara E, Nikolausz M, Harms H, Chatzinotas A (2010) Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil. Sci Total Environ 408:1221–1225

    Article  CAS  Google Scholar 

  • Kaur A, Chaudhary A, Kaur A, Choudhary R, Kaushik R (2005) Phospholipid fatty acid – a bioindicator of environment monitoring assessment in soil ecosystem. Curr Sci 89:1103–1112

    CAS  Google Scholar 

  • Kohler J, Caravaca F, Azcon R, Diaz G, Roldan A (2016) Suitability of the microbial community composition and function in a semiarid mine soil for assessing phytomanagement practices based on mycorrhizal inoculation and amendment addition. J Environ Manag 169:236–246

    Article  CAS  Google Scholar 

  • Kovacs A, Yacoby K, Gophna U (2010a) A systematic assessment of automated ribosomal intergenic spacer analysis (ARISA) as a tool for estimating bacterial richness. Res. Microbiology 161:192–197

    CAS  Google Scholar 

  • Kovacs A et al (2010b) Genotype is a stronger determinant than sex of the mouse gut microbiota. Microbial Ecol 1:6

    Google Scholar 

  • Lee DH, Zo YG, Kim SJ (1996) Nonradioactive method to study genetic profiles of natural bacterial communities by PCR–single-strand conformation polymorphism. Appl Environ Microbiol 62:3112–3120

    CAS  Google Scholar 

  • Lee CK, Barbier BK, Bottos EM, McDonald IR, Cary SC (2012) The Inter-Valley Soil Comparative Survey: the ecology of Dry Valley edaphic microbial communities. ISME J 6:1046–1057

    Article  CAS  Google Scholar 

  • Lester ED, Satomi M, Ponce A (2007) Microflora of extreme arid Atacama Desert soils. Soil Biol Biochem 39:704–708

    Article  CAS  Google Scholar 

  • Li X, Sun J, Wang H, Li X, Wang J, Zhang H (2017) Changes in the soil microbial phospholipid fatty acid profile with depth in three soil types of paddy fields in China. Geoderma 290:69–74

    Article  CAS  Google Scholar 

  • Likar M, Stres B, Rusjan D, Potisek M, Regvar M (2017) Ecological and conventional viticulture gives rise to distinct fungal and bacterial microbial communities in vineyard soils. Appl Soil Ecol 113:86–95

    Article  Google Scholar 

  • Liu Y, Wang P, Crowley D, Liu X, Chen J, Li L, Zheng J, Zhang X, Zheng J, Pan G (2016a) Methanogenic abundance and changes in community structure along a rice soil chronosequence from east China. Eur J Soil Sci. https://doi.org/10.1111/ejss.12348

    Article  CAS  Google Scholar 

  • Liu Y, Yang D, Zhang N, Chen L, Cui Z, Shen Q, Zhang R (2016b) Characterization of uncultured genome fragment from soil metagenomic library exposed rare mismatch of internal tetranucleotide frequency. Front Microbiol 7:2081

    Google Scholar 

  • Lukow T, Dunceld PF, Liesack W (2000) Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants. FEMS Microbiol Ecol 32:241–247

    Article  CAS  Google Scholar 

  • Lupwayi NZ, Lamey FJ, Blackshaw RE, Kanashiro DA, Pearson DC (2017) Phospholipid fatty acid biomarkers show positive soil microbial community responses to conservation soil management of irrigated crop rotations. Soil Tillage Res 168:1–10

    Article  Google Scholar 

  • Malghani S, Reim A, Von Fischer J, Conrad R, Kuebler K, Trumbore SE (2016) Soil methanotroph abundance and community composition are not influenced by substrate availability in laboratory incubations. Soil Biol Biochem 101:184–194

    Article  CAS  Google Scholar 

  • Manefield M, Whiteley AS, Griffiths RI, Bailey MJ (2002) RNA stable isotope probing, a novel means of linking microbial community function to phylogeny. Appl Environ Microbiol 68:5367–5373

    Article  CAS  Google Scholar 

  • Markowicz A, CycoĹ„ M, Piotrowska-Seget Z (2016) Microbial community structure and diversity in long-term hydrocarbon and heavy metal contaminated soils. Int J Environ Res 10:321–332

    CAS  Google Scholar 

  • Martin-Laurent F, Philippot L, Hallet S, Chaussod R, Germon JC, Soulas G, Catroux G (2001) DNA extraction from soils: old bias for new microbial diversity analysis methods. Appl Environ Microbiol 67:2354–2359

    Article  CAS  Google Scholar 

  • McGrath KC, Mondav R, Sintrajaya R, Slattery B, Schmidt S, Schenk PM (2010) Development of an environmental functional gene microarray for soil microbial communities. Appl Environ Microbiol 76:7161–7170

    Article  CAS  Google Scholar 

  • Mijangos I, Becerril JM, Albizu I, Epelde L, Garbisu C (2009) Effects of glyphosate on rhizosphere soil microbial communities under two different plant compositions by cultivation-dependent and independent methodologies. Soil Biol Biochem 41:505–513

    Article  CAS  Google Scholar 

  • Morales SE, Cosart T, Holben WE (2010) Bacterial gene abundances as indicators of greenhouse gas emission in soils. ISME J 4:799–808

    Article  CAS  Google Scholar 

  • Mossa AW, Dickinson MJ, West HM, Young SD, Crout NM (2017) The response of soil microbial diversity and abundance to long-term application of biosolids. Environ Pollut 224:16–25

    Article  CAS  Google Scholar 

  • Muyzer G (1999) DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol 2:317–322

    Article  CAS  Google Scholar 

  • Neufeld JD, Dumont MG, Vohra J, Murrell JC (2007) Methodological considerations for the use of stable isotope probing in microbial ecology. Microb Ecol 53:435–442

    Article  CAS  Google Scholar 

  • Nunan N, Daniell TJ, Singh BK, Papert A, McNicol JW, Prosser JI (2005) Links between plant and rhizoplane bacterial communities in grassland soils, characterized using molecular techniques. Appl Environ Microbiol 71:6784–6792

    Article  CAS  Google Scholar 

  • Ochsenreiter T, Selezi D, Quaiser A, Bonch-Osmolovskaya L, Schleper C (2003) Diversity and abundance of Crenarchaeota in terrestrial habitats studied by 16S RNA surveys and real time PCR. Environ Microbiol 5:787–797

    Article  CAS  Google Scholar 

  • Ogier JC, Son O, Gruss A, Tailliez P, Delacroix-Buchet A (2002) Identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis. Appl Environ Microbiol 68:3691–3701

    Article  CAS  Google Scholar 

  • Pernthaler A, Pernthaler J, Amann R (2002) Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria. Appl Environ Microbiol 68:3094–3101

    Article  CAS  Google Scholar 

  • Props R, Kerckhof FM, Rubbens P, De Vrieze J, Sanabria EH, Waegeman W, Monsieurs P, Hammes F, Boon N (2017) Absolute quantification of microbial taxon abundances. ISME J 11:584–587

    Article  Google Scholar 

  • Quemeneur M, Garrido F, Billard P, Breeze D, Leyyal C, Jauzeind M, Joulianb C (2016) Community structure and functional arrA gene diversity associated with arsenic reduction and release in an industrially contaminated soil. Geomicrobiol J 33:839–849

    Article  CAS  Google Scholar 

  • Sanzani SM, Nicosia MGLD, Faedda R, Cacciola SO, Schena L (2014) Use of quantitative PCR detection methods to study biocontrol agents and phytopathogenic fungi and oomycetes in environmental samples. J Phytopathol 162:1–13

    Article  CAS  Google Scholar 

  • Schadt CW, Zhou J (2005) Advances in microarrays for soil microbial community analyses. In: Nannipieri P, Smalla K (eds) Soil biology: nucleic acids and proteins in soil. Springer-Verlag, New York

    Google Scholar 

  • Scheinert P, Kruse R, Ullmann U, Söller R, Krupp G (1996) Molecular differentiation of bacteria by PCR amplification of the 16S-23S rRNA spacer. J Microbiol Methods 26:103–117

    Article  CAS  Google Scholar 

  • Schimak MP, Kleiner M, Wetzel S, Liebeke M, Dubilier N, Fuchsa BM (2016) MiL-FISH: multilabeled oligonucleotides for fluorescence in situ hybridisation improve visualisation of bacterial cells. Appl Environ Microbiol 82:62–70

    Article  CAS  Google Scholar 

  • Schwieger F, Tebbe CC (1998) A new approach to utilize PCR-single-strand-conformation polymorphism for 16S rRNA gene-based microbial community analysis. Appl Environ Microbiol 64:4870–4876

    CAS  Google Scholar 

  • Sekar R, Pernthaler A, Pernthaler J, Warnecke F, Posch T, Amann R (2003) An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization. Appl Environ Microbiol 69:2928–2935

    Article  CAS  Google Scholar 

  • Selvakumar N, Ding BC, Wilson SM (1997) Separation of DNA strands facilitates detection of point mutations by PCR-SSCP. BioTechniques 22:604–606

    Article  CAS  Google Scholar 

  • Smets W, Leff JW, Bradford MA, McCulley RL, Lebeer S, Fierer N (2016) A method for simultaneous measurement of soil bacterial abundances and community composition via 16S rRNA gene sequencing. Soil Biol Biochem 96:145–151

    Article  CAS  Google Scholar 

  • Smith CJ, Osborn AM (2009) Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol 67:6–20

    Article  CAS  Google Scholar 

  • Taylor JD, McKew BA, Kuhl A, McGenity TJ, Underwood GJC (2013) Microphytobenthic extracellular polymeric substances (EPS) in intertidal sediments fuelboth generalist and specialist EPS-degrading bacteria. Limnol Oceanogr 58:1463–1480

    Article  CAS  Google Scholar 

  • Teira E, Reinthaler T, Pernthaler A, Pernthaler J, Herndl GJ (2004) Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean. Appl Environ Microbiol 70:441–4414

    Article  CAS  Google Scholar 

  • Wang M, Liu P, Xiong W, Zhou Q, Wangxiao J, Zeng Z, Sun Y (2018) Fate of potential indicator antimicrobial resistance genes (ARGs) and bacterial community diversity in simulated manure-soil microcosms. Ecotoxicol Environ Saf 147:817–823

    Article  CAS  Google Scholar 

  • Widjojoatmodjo MN, Fluit AC, Verhoef J (1995) Molecular identification of bacteria by fluorescence-based PCR–single-strand conformation polymorphism analysis of the 16S rRNA gene. J Clin Microbiol 33:2601–2606

    CAS  Google Scholar 

  • Wilkinson SC, Anderson JM, Scardelis SP, Tisiafouli M, Taylor A, Wolters V (2002) PLFA profiles of microbial communities in decomposing conifer litters subject to moisture stress. Soil Biol Biochem 34:189–200

    Article  CAS  Google Scholar 

  • Wu C, Wang W, Wang K, Li X, Qiu W, Li W (2016) Phospholipids fatty acids analysis of microbial communities in sewage sludge composting with inorganic bulking agent. Desalin Water Treat 57:27181–27190

    Article  CAS  Google Scholar 

  • Xue K, Wu L, Deng Y, He Z, Nostrand JV, Robertson PG, Schmidt TM, Zhou J (2013) Functional gene differences in soil microbial communities from conventional, low-input, and organic farmlands. Appl Environ Microbiol 79:1284–1292

    Article  CAS  Google Scholar 

  • Zelles L (1999) Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review. Biol Fertil Soils 29:111–129

    Article  CAS  Google Scholar 

  • Zhou JZ, Thompson DK (2002) Challenges in applying microarrays to environmental studies. Curr Opin Biotechnol 13:204–207

    Article  CAS  Google Scholar 

  • Zoppini A, Ademollo N, Amalfitano S, Capri S, Casella P, Fazi S, Marxen J, Patrolecco L (2016) Microbial responses to polycyclic aromatic hydrocarbon contamination in temporary river sediments: experimental insights. Sci Total Environ 541:1364–1371

    Article  CAS  Google Scholar 

  • Zornoza R, Acosta JA, Faz A, Baath E (2016) Microbial growth and community structure in acid mine soils after addition of different amendments for soil reclamation. Geoderma 272:64–72

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Environment & Sustainable Development, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Rama Kant Dubey, Vishal Tripathi, Rajan Chaurasia & Purushothaman Chirakkuzhyil Abhilash

  2. Chhattisgarh Swami Vivekananda Technical University, Bhilai, Chhattisgarh, India

    Ratna Prabha

  3. ICAR-National Bureau of Agriculturally Important Microorganisms, Mau Nath Bhanjan, Uttar Pradesh, India

    Dhananjaya Pratap Singh

  4. National Academy of Agricultural Research Management, Hyderabad, Telangana, India

    Ch. Srinivasa Rao

  5. Department of Applied Biology, University of Sharjah, Sharjah, United Arab Emirates

    Ali El-Keblawy

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  1. Rama Kant Dubey
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Dubey, R.K. et al. (2020). Methods for Exploring Soil Microbial Diversity. In: Unravelling the Soil Microbiome. SpringerBriefs in Environmental Science. Springer, Cham. https://doi.org/10.1007/978-3-030-15516-2_3

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