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Biomimetic Lipid Membranes: Fundamentals, Applications, and Commercialization pp 29–46Cite as

To Image the Orientation and Spatial Distribution of Reconstituted Na+,K+-ATPase in Model Lipid Membranes

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Abstract

Imaging of sub-optical dynamic features, such as functional membrane nanodomains that are short-lived and proteins that are embedded in such nanodomains, is a challenge with the currently available imaging techniques because such features of interests are very dynamic. Our approach to image dynamic suboptical features is based on a GUV-collapse method followed by high-resolution imaging. We have functionally reconstituted Na+,K+-ATPase, a P-type transmembrane ATPase protein, into free-standing giant unilamellar vesicles (GUVs) which are collapsed to form planar lipid bilayer (PLB) patches within the ∼10 ms time scale. Using our method, we have successfully imaged the PLB patches using atomic force microscopy under physiological conditions to quantify orientation and density of Na+,K+-ATPase in membrane with nanoscopic domains.

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Acknowledgement

TB acknowledges Prof. John H. Ipsen, Prof. O.G. Mouritsen, A.C. Simonsen (SDU), P.L. Hansen, L.A. Bagatolli, J. Brewer (SDU), L. Duelund, B. Franchi and H. Kidmose for useful discussions on the work presented in this chapter.

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Authors and Affiliations

  1. Max Planck Institute of Colloids and Interfaces, Golm, Potsdam, Germany

    Tripta Bhatia

  2. Department of Biomedicine, Aarhus University, Aarhus, Denmark

    Flemming Cornelius

Authors
  1. Tripta Bhatia
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  2. Flemming Cornelius
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Correspondence to Tripta Bhatia .

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Editors and Affiliations

  1. Department of Molecular Biology and Genetics, İstanbul Technical University, Istanbul, Turkey

    Fatma N. Kök

  2. Department of Bioengineering, Izmir Institute of Technology, Urla, Izmir, Turkey

    Ahu Arslan Yildiz

  3. School of Medicine, Stanford University, Palo Alto, CA, USA

    Fatih Inci

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Bhatia, T., Cornelius, F. (2019). To Image the Orientation and Spatial Distribution of Reconstituted Na+,K+-ATPase in Model Lipid Membranes. In: Kök, F., Arslan Yildiz, A., Inci, F. (eds) Biomimetic Lipid Membranes: Fundamentals, Applications, and Commercialization. Springer, Cham. https://doi.org/10.1007/978-3-030-11596-8_2

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