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Incorporation of Non-Canonical Amino Acids

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Novel Chemical Tools to Study Ion Channel Biology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 869))

Abstract

In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.

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Authors and Affiliations

  1. Department of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Road, 52246, Iowa City, IA, USA

    Lilia Leisle & Christopher A. Ahern

  2. Department of Physiology and Pharmacology, Oregon Health and Sciences University, 97239, Portland, OR, USA

    Francis Valiyaveetil

  3. Department of Biochemistry and Biophysics, Oregon State University Corvallis, 97331, Corvallis, OR, USA

    Ryan A. Mehl

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  1. Lilia Leisle
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  2. Francis Valiyaveetil
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  3. Ryan A. Mehl
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  4. Christopher A. Ahern
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Correspondence to Christopher A. Ahern .

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  1. Molecular Physiology and Biophysics, University of Iowa, IOWA CITY, Iowa, USA

    Christopher Ahern

  2. Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark

    Stephan Pless

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Leisle, L., Valiyaveetil, F., Mehl, R., Ahern, C. (2015). Incorporation of Non-Canonical Amino Acids. In: Ahern, C., Pless, S. (eds) Novel Chemical Tools to Study Ion Channel Biology. Advances in Experimental Medicine and Biology, vol 869. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2845-3_7

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