Abstract
Viral inclusion bodies have long been considered pathognomoic. Coronaviruses cause the formation of cytoplasmic inclusions consisting of densely staining particles contained within a granular matrix enclosed by a double membrane (1). During studies on the assembly of 229E in human embryomic lung fibroblasts roughly circular electron lucent structures with dense limiting membranes, sometimes empty but frequently containing net-like beaded strands, were seen in the cytoplasm of infected cells that differed from the previously described inclusions. These structures vary in size from 300–900nm in diameter (Fig. 1) and appear at approximately 6hr post-infection (PI), i.e. at a time when viral protein synthesis is initiated (2) and before the assembly of virions (3).
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
L.S. Oshiro, J.H. Schieble, and E.H. Lennette, Electron Microscopie Studies of Coronavirus, J. Gen Virol. 12:161(1971).
M.C. Kemp, J.C. Hierholzer, A. Harrison, and J.S. Burks, Characterization of Viral Proteins Synthesized in 229E Infected Cells and Effect(s) of Inhibition of Glycosylcation and Glycoprotein Transport on This Process (See these proceedings).
M.C. Kemp, A. Harrison, J.C. Hierholzer, and J.S. Burks, Assembly of 229E Virions in Human Embryonic Lung Fibroblasts and Effects of Inhibition of Glycosylation and Glycoprotein Transport on This Process (See these proceedings).
J.S. Burks, B. L. DeVald, L.D. Jankovsky, and J.C. Gerdes, Two Coronaviruses Isolated from Central Nervous System of Two Multiple Sclerosis Patients, Science 209:933 (1980).
P.M. Grimley, I. K. Berezesky, and R.M. Friedman, Cytoplasmic Structures Associated with an Arbovirus Infection: Loci of Viral Ribonucleic Acid Synthesis, J. Virol. 2:1326 (1968).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Kemp, M.C., Harrison, A., Hierholzer, J.C., Burks, J.S. (1984). Electron Lucent Structures Induced by Coronaviruses. In: Rottier, P.J.M., van der Zeijst, B.A.M., Spaan, W.J.M., Horzinek, M.C. (eds) Molecular Biology and Pathogenesis of Coronaviruses. Advances in Experimental Medicine and Biology, vol 173. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9373-7_15
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9373-7_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-9375-1
Online ISBN: 978-1-4615-9373-7
eBook Packages: Springer Book Archive