Abstract
The genetic variation in equine arteritis virus (EAV) nonstructural (NS) protein-encoding open reading frames (ORF) 3 and 4 genes was investigated. Nucleotide and deduced amino acid sequences from seven different EAV isolates (one European, one American and five Canadian isolates) and the Arvac vaccine strain were compared with those of the Bucyrus reference strain. ORF 3 nucleotide and amino acid sequence identities amongst these isolates (including the Arvac vaccine strain) and the Bucyrus reference strain ranged from 85.6 to 98.8%, and 85.3 to 98.2%, respectively, whereas ORF 4 nucleotide and amino acid sequence identities ranged from 90.4 to 98.3%, and 90.8 to 97.4%, respectively. Phylogenetic tree analysis based on the ORF 3 nucleotide sequences showed that the European Vienna isolate could be classified into a genetically divergent group from all other isolates and the Arvac vaccine strain. In contrast, a phylogenetic relationship among all EAV isolates and the Arvac vaccine strain based on the ORF 4 nucleotide sequences was observed.
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Keywords
- Amino Acid Sequence Identity
- Nucleotide Sequence Identity
- Amino Acid Sequence Analysis
- Venezuelan Equine Encephalitis Virus
- Equine Arteritis Virus
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References
Balasuriya U.B.R., MacLachlan N.J., De Vries A.A.F., Rossito P.V. and Rottier P.J.M., 1995, Identification of a neutralization site in the major envelope glycoprotein (GL) of equine arteritis virus, Virology 207: 518–527.
Balasuriya U.B.R., Timoney P.J., McCollum W.H. and MacLachlan N.J., 1995, Phylogenetic analysis of open reading frame 5 of field isolates of equine arteritis virus and identification of conserved and nonconserved regions in the GL envelope glycoprotein, Virology 214: 690–697.
Chirnside E.D., Wearing CM., Binns M.M., Mumford J. A., 1994, Comparison of M and N gene sequences distinguishes variation amongst equine arteritis virus isolates, J. Gen. Virol. 75: 1491–1497.
Chirnside E.D., De Vries A.A.F., Mumford J.A. and Rottier P.J.M., 1995, Equine arteritis virus-neutralizing antibody in the horse is induced by a determinant on the large glycoprotein GL, J. Gen. Virol. 76: 1989–1998.
Den Boon J.A., Snijder E.J., Chirnside E.D., De Vries A.A.F., Horzinek M.C. and Spann W.J.M., 1991, Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily, J. Virol. 65: 2910–2920.
Deregt D., De Vries A.A.F., Raamsman M.J.B. and Elmgren L.D., 1994, Monoclonal antibodies to equine arteritis virus protein identify the GL protein as a target for virus neutralization. J. Gen. Virol. 75: 2439–2444.
De Vries A. A.F., Chirnside E.D., Horzinek M.C. and Rottier P.J.M., 1992, Structural proteins of equine arteritis virus,J. Virol. 66: 6294–6303.
Felsenstein J., 1993, PHYLIP (Phylogenic Inference Package) 3.5c Manual, University of Washington, Department of Genetics SK-50, Seattle, WA98195.
Glaser A.L., De Vries A.A.F., Dubovi E. J., 1995, Comparison of equine arteritis virus isolates using neutralizing monoclonal antibodies and identification of sequence changes in GL associated with neutralization resistance, J. Gen. Virol. 76: 2223–2233.
Godeny E.K., Chen L., Kumar S.N., Methven S.L., Koonin E.V. and Binton M.A., 1993, Complete genomic sequence and phylogenetic analysis of the lactate dehydrogenase-elevating virus (LDV), Virology 194: 585–596.
Hedges, J.F., Balasuriya U.B.R., Timoney P.J., McCollum W.H. and MacLachlan N.J., 1996, Genetic variation in open reading frame 2 of field isolate and laboratory strains of equine arteritis virus, Vir. Res. 42: 41–52.
Lepage N., St-Laurent G., Carman S. and Archambault D., 1996, Comparison of nucleic and amino acid sequences and phylogenic analysis of the Gs protein of various equine arteritis virus isolates, Virus Genes 13: 87–91.
Murphy T.W., McCollum W.H., Timoney P.J., Klingeborn B.W., Hyllseth B., Golnik W. and Erasmus B., 1992, Genomic variability among globally distributed isolates of equine arteritis virus, Vet. Microbiol. 32: 101–115.
Oberste M.S., Parker M.D. and Smith J.F., 1996, Complete sequence of Venezuelan equine encephalitis virus subtype IE reveals conserved and hypervariable domains within the C terminus of nsP3, Virology 219: 314–320.
Rao CD., Das M., Ilango P., Lalwani R., Bhargavi S.R. and Gowda K., 1995, Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP 3, Virology 207:327–333.
Sanger F., Nicklen S. and Coulson A.R., 1977, DNA sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sci. USA 74: 5463–5467.
St-Laurent G., Morin G. and Archambault D., 1994, Detection of equine arteritis virus following amplification of structural and nonstructural viral genes by reverse transcription-PCR, J. Clin. Microbiol. 32: 658–665.
St-Laurent G., Lepage N., Carman S. and Archambault D., 1997, Genetic and amino acid analysis of the GL protein of Canadian, American and European equine arteritis virus isolates, Can. J. Vet. Res. 61: 72–76.
Timoney P.J. and McCollum W.H., 1993, Equine viral arteritis, Vet. Clinics North Amer.: Equine practice 9: 295–309.
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Archambault, D., Laganière, G., St-Laurent, G. (1998). Genetic Variation and Phylogenetic Analysis of Open Reading Frames 3 and 4 of Various Equine Arteritis Virus Isolates. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_106
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_106
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