Abstract
The availability of infectious full-length cDNA clones is important for the molecular genetic analysis of the structure and function of RNA virus genomes (Ahlquist et al, 1984; Boursnell et al., 1987). Infectious cDNA clones for a number of positive-stranded RNA viruses have been developed, advancing our understanding of the molecular mechanisms of viral replication and pathogenesis, and has resulted in novel approaches for heterologous gene expression and vaccine development. Clearly, a full-length infectious construct of TGEV would enhance our understanding of all aspects of TGEV biology by providing a means for reverse genetic analysis.
Keywords
- Infectious Bronchitis Virus
- Infectious Clone
- Baby Hamster Kidney Cell
- Infectious cDNA Clone
- Transmissible Gastroenteritis Virus
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Yount, B., K. M. Curtis, and R. S. Baric. A simple strategy for the systematic assembly of large RNA and DNA genomes: the transmissible gastroenteritis virus model. submitted
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Curtis, K.M., Yount, B., Baric, R.S. (2001). A Simple Strategy to Assemble Infectious RNA and DNA Clones. In: Lavi, E., Weiss, S.R., Hingley, S.T. (eds) The Nidoviruses. Advances in Experimental Medicine and Biology, vol 494. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1325-4_69
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DOI: https://doi.org/10.1007/978-1-4615-1325-4_69
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