Abstract
Oxidative stress is a node common to the causes and effects of various ocular diseases. We have shown that thioredoxin has neuroprotective effects on tubby photoreceptors. We also demonstrated that nanoceria (cerium oxide nanoparticles), which are direct antioxidants, have long-term effects on prevention of retinal degeneration in tubby mice. Here, using commercially available PCR array plates, we surveyed the regulation in expression of 89 oxidative stress-associated genes in the eyes of P12 tubby mice which are either intravitreally injected with nanoceria or in which the Trx gene is overexpressed. Our data demonstrate that nanoceria and Trx regulate the same group of genes associated with antioxidative stress and antioxidant defense.
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Abbreviations
- Trx:
-
Thioredoxin
- ASK1:
-
Apoptosis signal kinase 1
- MAPKs:
-
Mitogen-activated protein kinases
- Nanoceria:
-
Cerium oxide nanoparticles
- P7:
-
Postnatal day 7
- Wt:
-
Wild type
- IACUC:
-
Institutional Animal Care and Use Committee
- Nrf2:
-
Nuclear factor erythroid 2-related factor
- siRNA:
-
Small interfering RNA
- ROS:
-
Reactive oxygen species
- ICAM-1:
-
Intercellular adhesion molecule 1
- HMVEC:
-
Human microvascular endothelial cells
- Nxn:
-
Nucleoredoxin
- Cys:
-
Cysteine residue
- Wnt:
-
Wingless/Intergration, Wg/Int
- Dvl:
-
Dishevelled
- RPE:
-
Retinal pigment epithelium
- bZIP:
-
Basic leucine zipper
- ARACNE:
-
Algorithm for the Reconstruction of Accurate Cellular Networks
- CLR:
-
Context Likelihood of Relatedness
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Acknowledgment
This review was written with the partial support of NIH grant COBRE-P20 RR017703, R01EY018724, R01EY022111.
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Cai, X., Yodoi, J., Seal, S., McGinnis, J. (2014). Nanoceria and Thioredoxin Regulate a Common Antioxidative Gene Network in tubby Mice. In: Ash, J., Grimm, C., Hollyfield, J., Anderson, R., LaVail, M., Bowes Rickman, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 801. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3209-8_104
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DOI: https://doi.org/10.1007/978-1-4614-3209-8_104
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