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The Acceptor Quinones of Purple Photosynthetic Bacteria — Structure and Spectroscopy

  • Chapter
The Purple Phototrophic Bacteria

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 28))

Summary

Type II reaction centers (RCs) have two acceptor quinones that act in series. The primary quinone, QA, cycles between the oxidized quinone and singly reduced semiquinone. QA is tightly bound to the protein as aprosthetic group. The secondary quinone, QB, is reduced by Q −A , first to the semiquinone and then to the doubly reduced, fully protonated quinol, QH2. QB freely associates with the protein in the quinone and quinol states. The properties of the two quinones that facilitate this process are largely determined by the nature of the two quinone binding sites. Many reaction center crystal structures show these interactions, although there are significant uncertainties in the conformations of the two quinones, and in the significance of the variable location of QB in the protein. Consequently the influence of structure on quinone function is only very crudely understood. These issues are discussed with emphasis on the quinone reactions in the reaction center from the photosynthetic bacterium, Rhodobacter (Rba.) sphaeroides, which is the best characterized. The structural features of the quinones and their local protein environments are examined in the light of extensive spectroscopic studies, especially by Fourier transform infra-red spectroscopy (FTIR), electron paramagnetic resonance (EPR) and electron nuclear double resonance (ENDOR), on the quinones in their functional redox states.

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Abbreviations

BChl:

bacteriochlorophyll

Bphe:

bacteriopheophytin

Blc. :

Blastochloris

DFT:

density functional theory

ENDOR:

Electron Nuclear Double Resonance

EPR:

Electron Paramagnetic Resonance

FTIR:

Fourier Transform Infrared Spectroscopy

IR:

infra-red

NMR:

Nuclear Magnetic Resonance

QA,B :

primary and secondary quinone acceptors

Rba. :

Rhodobacter

RC:

reaction center

UQ:

ubiquinone (2,3-dimethoxy -5-methyl-6-isoprenyl-1,4-benzoquinone)

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Authors and Affiliations

  1. Department of Biochemistry, and Center for Biophysics & Computational Biology, University of Illinois, Urbana, IL, 61801, USA

    Colin A. Wraight

  2. Department of Physics, City College of New York, New York, NY, 10031, USA

    Marilyn R. Gunner

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Correspondence to Colin A. Wraight .

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  1. University of Sheffield, UK

    C. Neil Hunter

  2. University of Pennsylvania, USA

    Fevzi Daldal

  3. Argonne National Laboratory, USA

    Marion C. Thurnauer

  4. University of British Columbia, Canada

    J. Thomas Beatty

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Wraight, C.A., Gunner, M.R. (2009). The Acceptor Quinones of Purple Photosynthetic Bacteria — Structure and Spectroscopy. In: Hunter, C.N., Daldal, F., Thurnauer, M.C., Beatty, J.T. (eds) The Purple Phototrophic Bacteria. Advances in Photosynthesis and Respiration, vol 28. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8815-5_20

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