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Dynamic Nuclear Polarization: New Methodology and Applications

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NMR of Proteins and Small Biomolecules

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 326))

Abstract

One way to overcome the intrinsically low sensitivity of Nuclear Magnetic Resonance spectroscopy is to enhance the signal by dynamic nuclear polarization (DNP), where the polarization of high-gyromagnetic ratio (γ) electrons is transferred to the surrounding nuclei using microwave (MW) irradiation. Recent developments in DNP instrumentations and applications have shown that DNP is one of the most effective methods to increase the nuclear spin polarization in inorganic, organic, and biological materials. It is possible to obtain a solution of molecules containing hyperpolarized nuclei in combination with methods to dissolve rapidly the polarized solid sample. In this chapter, a brief introduction on a theoretical basis and some of new DNP applications in NMR spectroscopy as well as medical applications in Magnetic Resonance Imaging (MRI) are described.

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References

  1. Overhauser AW (1953) Phys Rev 92:411–415

    Article  CAS  Google Scholar 

  2. Carver TR, Slichter CP (1953) Phys Rev 92:212–213

    Article  CAS  Google Scholar 

  3. Carver TR, Slichter CP (1956) Phys Rev 102:975–980

    Article  CAS  Google Scholar 

  4. Abragam A, Proctor WG (1958) Phys Rev 109:1441–1448

    Article  Google Scholar 

  5. Duckett SB, Sleigh CJ (1999) Prog Nucl Magn Reson Spectrosc 34:71–92

    Article  CAS  Google Scholar 

  6. Bargon JNaJ (1997) Prog Nucl Magn Reson Spectrosc 31:293–315

    Article  Google Scholar 

  7. Navon G, Song Y-Q, Rõõm T, Appelt S, Taylor RE, Pines A (1996) Science 271:1848–1851

    Article  CAS  Google Scholar 

  8. Fitzgerald RJ, Sauer KL, Happer W (1998) Chem Phys Lett 284:87–92

    Article  CAS  Google Scholar 

  9. Cherubini A, Payne GS, Leach MO, Bifone A (2003) Chem Phys Lett 371:640–644

    Article  CAS  Google Scholar 

  10. Pavlovskaya GE, Cleveland ZI, Stupic KF, Basaraba RJ, Meersmann T (2005) Proc Natl Acad Sci USA 102:18275–18279

    Article  CAS  Google Scholar 

  11. Loening NM, Rosay M, Weis V, Griffin RG (2002) J Am Chem Soc 124:8808–8809

    Article  CAS  Google Scholar 

  12. Hofer P, Parigi G, Luchinat C, Carl P, Guthausen G, Reese M, Carlomagno T, Griesinger C, Bennati M (2008) J Am Chem Soc 130:3254–3255

    Article  Google Scholar 

  13. Maly T, Debelouchina GT, Bajaj VS, Hu KN, Joo CG, Mak-Jurkauskas ML, Sirigiri JR, van der Wel PCA, Herzfeld J, Temkin RJ, Griffin RG (2008) J Chem Phys 128:052211

    Article  Google Scholar 

  14. Armstrong BD, Han S (2007) J Chem Phys 127:104508

    Article  Google Scholar 

  15. Grucker D, Guiberteau T, Eclancher B, Chambron J, Chiarelli R, Rassat A, Subra G, Gallez B (1995) J Magn Reson B 106:101–109

    Article  CAS  Google Scholar 

  16. Abragam A, Goldman M (1978) Rep Prog Phys 41:395

    Article  CAS  Google Scholar 

  17. Henstra A, Dirksen P, Wenckebach WT (1988) Phys Lett A 134:134–136

    Article  CAS  Google Scholar 

  18. Wollan DS (1976) Phys Rev B 13:3671–3685

    Article  CAS  Google Scholar 

  19. Farrar CT, Hall DA, Gerfen GJ, Inati SJ, Griffin RG (2001) J Chem Phys 114:4922–4933

    Article  CAS  Google Scholar 

  20. Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG (1997) Science 276:930–932

    Article  CAS  Google Scholar 

  21. Hu KN, Yu HH, Swager TM, Griffin RG (2004) J Am Chem Soc 126:10844–10845

    Article  CAS  Google Scholar 

  22. Hu KN, Bajaj VS, Rosay M, Griffin RG (2007) J Chem Phys 126:044512

    Google Scholar 

  23. Song CS, Hu KN, Joo CG, Swager TM, Griffin RG (2006) J Am Chem Soc 128:11385–11390

    Article  CAS  Google Scholar 

  24. Bajaj VS, Hornstein MK, Kreischer KE, Sirigiri JR, Woskov PP, Mak-Jurkauskas ML, Herzfeld J, Temkin RJ, Griffin RG (2007) J Magn Reson 189:251–279

    Article  CAS  Google Scholar 

  25. van der Wel PCA, Hu KN, Lewandowski J, Griffin RG (2006) J Am Chem Soc 128:10840–10846

    Article  Google Scholar 

  26. Pines A, Gibby MG, Waugh JS (1972) J Chem Phys 56:1776–1777

    Article  CAS  Google Scholar 

  27. Conte P, Spaccini R, Piccolo A (2004) Prog Nucl Magn Reson Spectrosc 44:215–223

    Article  CAS  Google Scholar 

  28. Henstra A, Lin T-S, Schmidt J, Wenckebach WT (1990) Chem Phys Lett 165:6

    Article  CAS  Google Scholar 

  29. Schmidt J, van den Heuvel DJ, Henstra A, Lin T-S, Wenckebach WT (1992) Isr J Chem 32:165

    CAS  Google Scholar 

  30. Schmidt J, van den Heuvel DJ, Henstra A, Lin T-S, Wenckebach WT (1992) Pure Appl Chem 64:859

    Article  CAS  Google Scholar 

  31. van den Heuvel DJ, Henstra A, Lin T-S, Schmidt J, Wenckebach WT (1992) Chem Phys Lett 188:194–200

    Article  Google Scholar 

  32. Reynhardt CE, High GL (1998) J Chem Phys 109:4100

    Article  CAS  Google Scholar 

  33. Takeda K, Takegoshi K, Terao T (2001) Chem Phys Lett 345:166–170

    Article  CAS  Google Scholar 

  34. Weis V, Griffin RG (2006) Solid State Nucl Magn Reson 29:66–78

    Article  CAS  Google Scholar 

  35. Ardenkjaer-Larsen JH, Laursen I, Leunbach I, Ehnholm G, Wistrand LG, Petersson JS, Golman K (1998) J Magn Reson 133:1–12

    Article  CAS  Google Scholar 

  36. Reddy TJ, Iwama T, Halpern HJ, Rawal VH (2002) J Org Chem 67:4635–4639

    Article  CAS  Google Scholar 

  37. Wind RA, Duijvestijn MJ, Vanderlugt C, Manenschijn A, Vriend J (1985) Prog Nucl Magn Reson Spectrosc 17:33–67

    Article  CAS  Google Scholar 

  38. Becerra LR, Gerfen GJ, Temkin RJ, Singel DJ, Griffin RG (1993) Phys Rev Lett 71:3561–3564

    Article  CAS  Google Scholar 

  39. Afeworki M, McKay RA, Schaefer J (1992) Macromolecules 25:4084

    Article  CAS  Google Scholar 

  40. Ardenkjaer-Larsen JH, Fridlund B, Gram A, Hansson G, Hansson L, Lerche MH, Servin R, Thaning M, Golman K (2003) Proc Natl Acad Sci USA 100:10158–10163

    Article  CAS  Google Scholar 

  41. Granatstein VL, Parker RK, Armstrong CM (1999) Proc IEEE 87:702

    Article  Google Scholar 

  42. Abrams RH, Levush B, Mondelli AA, Parker RK (2001) IEEE Microw Mag 3:61–72

    Article  Google Scholar 

  43. Parker RK, Abrams RH Jr, Danly BG, Levush B (2002) Microwave Theory and Technique 50:835–845

    Article  Google Scholar 

  44. Woskov PP, Bajaj VS, Hornstein MK, Temkin RJ, Griffin RG (2005) IEEE Trans Microw Theory Tech 53:1863–1869

    Article  Google Scholar 

  45. McCarney ER, Armstrong BD, Lingwood MD, Han S (2007) Proc Natl Acad Sci USA 104:1754–1759

    Article  CAS  Google Scholar 

  46. Dorn HC, Wang J, Allen L, Sweeney D, Glass TE (1988) J Magn Reson 79:404–412

    CAS  Google Scholar 

  47. Hyde JS (1965) J Chem Phys 43:1806

    Article  CAS  Google Scholar 

  48. Lesage A, Lelli M, Gajan D, Caporini MA, Vitzthum V, Mieville P, Alauzun J, Roussey A, Thieuleux C, Mehdi A, Bodenhausen G, Coperet C, Emsley L (2010) J Am Chem Soc 132:15459–15461

    Article  CAS  Google Scholar 

  49. Lelli M, Gajan D, Lesage A, Caporini MA, Vitzthum V, Mieville P, Heroguel F, Rascon F, Roussey A, Thieuleux C, Boualleg M, Veyre L, Bodenhausen G, Coperet C, Emsley L (2011) J Am Chem Soc 133:2104–2107

    Article  CAS  Google Scholar 

  50. Zeng HF, Lee Y, Hilty C (2010) Anal Chem 82:8897–8902

    Article  CAS  Google Scholar 

  51. Hilty C, Bowen S (2010) Org Biomol Chem 8:3361–3365

    Article  CAS  Google Scholar 

  52. Lerche MH, Meier S, Jensen PR, Hustvedt SO, Karlsson M, Duus JO, Ardenkjaer-Larsen JH (2011) NMR Biomed 24:96–103

    Article  CAS  Google Scholar 

  53. Gloggler S, Emondts M, Colell J, Muller R, Blumich B, Appelt S (2011) Analyst 136:1566–1568

    Article  Google Scholar 

  54. Lerche MH, Meier S, Jensen PR, Baumann H, Petersen BO, Karlsson M, Duus JO, Ardenkjaer-Larsen JH (2010) J Magn Reson 203:52–56

    Article  CAS  Google Scholar 

  55. Harris T, Giraudeau P, Frydman L (2011) Chem Eur J 17:697–703

    Article  CAS  Google Scholar 

  56. Bajaj VS, Mak-Jurkauskas ML, Belenky M, Herzfeld J, Griffin RG (2010) J Magn Reson 202:9–13

    Article  CAS  Google Scholar 

  57. Salnikov E, Rosay M, Pawsey S, Ouari O, Tordo P, Bechinger B (2010) J Am Chem Soc 132:5940

    Article  CAS  Google Scholar 

  58. Vukojevic V, Bowen AM, Wilhelm K, Ming Y, Ce Z, Schleucher J, Hore PJ, Terenius L, Morozova-Roche LA (2010) Langmuir 26:14782–14787

    Article  CAS  Google Scholar 

  59. Janssen GJ, Daviso E, van Son M, de Groot HJM, Alia A, Matysik J (2010) Photosynth Res 104:275–282

    Article  CAS  Google Scholar 

  60. Bajaj VS, Mak-Jurkauskas ML, Belenky M, Herzfeld J, Griffin RG (2009) Proc Natl Acad Sci USA 106:9244–9249

    Article  CAS  Google Scholar 

  61. Hu KN, Song C, Yu HH, Swager TM, Griffin RG (2008) J Chem Phys 128:052302

    Article  Google Scholar 

  62. Akbey U, Franks WT, Linden A, Lange S, Griffin RG, van Rossum BJ, Oschkinat H (2010) Angew Chem Int Ed Engl 49:7803–7806

    Article  CAS  Google Scholar 

  63. Maly T, Andreas LB, Smith AA, Griffin RG (2010) Phys Chem Chem Phys 12:5872–5878

    Article  CAS  Google Scholar 

  64. Gallagher FA, Kettunen MI, Brindle KM (2009) Prog Nucl Magn Reson Spectrosc 55:285–295

    Article  CAS  Google Scholar 

  65. Warren WS, Jenista E, Branca RT, Chen X (2009) Science 323:1711–1714

    Article  CAS  Google Scholar 

  66. Golman K, Zandt RI, Lerche M, Pehrson R, Ardenkjaer-Larsen JH (2006) Cancer Res 66:10855–10860

    Article  CAS  Google Scholar 

  67. Merritt ME, Harrison C, Storey C, Jeffrey FM, Sherry AD, Malloy CR (2007) Proc Natl Acad Sci USA 104:19773–19777

    Article  CAS  Google Scholar 

  68. Chen AP, Albers MJ, Cunningham CH, Kohler SJ, Yen YF, Hurd RE, Tropp J, Bok R, Pauly JM, Nelson SJ, Kurhanewicz J, Vigneron DB (2007) Magn Reson Med 58:1099–1106

    Article  CAS  Google Scholar 

  69. Kohler SJ, Yen Y, Wolber J, Chen AP, Albers MJ, Bok R, Zhang V, Tropp J, Nelson S, Vigneron DB, Kurhanewicz J, Hurd RE (2007) Magn Reson Med 58:65–69

    Article  CAS  Google Scholar 

  70. Albers MJ, Bok R, Chen AP, Cunningham CH, Zierhut ML, Zhang VY, Kohler SJ, Tropp J, Hurd RE, Yen YF, Nelson SJ, Vigneron DB, Kurhanewicz J (2008) Cancer Res 68:8607–8615

    Article  CAS  Google Scholar 

  71. Nelson SJ, Vigneron D, Kurhanewicz J, Chen A, Bok R, Hurd R (2008) Appl Magn Reson 34:533–544

    Article  CAS  Google Scholar 

  72. Day SE, Kettunen MI, Gallagher FA, Hu DE, Lerche M, Wolber J, Golman K, Ardenkjaer-Larsen JH, Brindle KM (2007) Nat Med 13:1382–1387

    Article  CAS  Google Scholar 

  73. Merritt ME, Harrison C, Storey C, Sherry AD, Malloy CR (2008) Magn Reson Med 60:1029–1036

    Article  CAS  Google Scholar 

  74. Schroeder MA, Cochlin LE, Heather LC, Clarke K, Radda GK, Tyler DJ (2008) Proc Natl Acad Sci USA 105:12051–12056

    Article  CAS  Google Scholar 

  75. Golman K, Petersson JS, Magnusson P, Johansson E, Akeson P, Chai CM, Hansson G, Mansson S (2008) Magn Reson Med 59:1005–1013

    Article  CAS  Google Scholar 

  76. Schroeder MA, Atherton HJ, Cochlin LE, Clarke K, Radda GK, Tyler DJ (2009) Magn Reson Med 61:1007–1014

    Article  CAS  Google Scholar 

  77. Adrogue HJ, Madias NE (1998) N Engl J Med 338:26–34

    Article  CAS  Google Scholar 

  78. Grinstein S, Swallow CJ, Rotstein OD (1991) Clin Biochem 24:241–247

    Article  CAS  Google Scholar 

  79. Hohn-Berlage M, Okada Y, Kloiber O, Hossmann KA (1989) NMR Biomed 2:240–245

    Article  CAS  Google Scholar 

  80. Gillies RJ, Raghunand N, Garcia-Martin ML, Gatenby RA (2004) IEEE Eng Med Biol Mag 23:57–64

    Article  Google Scholar 

  81. Raghunand N, He X, van Sluis R, Mahoney B, Baggett B, Taylor CW, Paine-Murrieta G, Roe D, Bhujwalla ZM, Gillies RJ (1999) Br J Cancer 80:1005–1011

    Article  CAS  Google Scholar 

  82. Raghunand N, Mahoney B, van Sluis R, Baggett B, Gillies RJ (2001) Neoplasia (New York, NY) 3:227–235

    Google Scholar 

  83. Gallagher FA, Kettunen MI, Day SE, Hu DE, Ardenkjaer-Larsen JH, in\primet Zandt R, Jensen PR, Karlsson M, Golman K, Lerche MH, Brindle KM (2008) Nature 453:940–U973

    Article  CAS  Google Scholar 

  84. Ludwig C, Marin-Montesinos I, Saunders MG, Emwas AH, Pikramenou Z, Hammond SP, Gunther UL (2010) Phys Chem Chem Phys 12:5868–5871

    Article  CAS  Google Scholar 

  85. Panek R, Granwehr J, Leggett J, Kockenberger W (2010) Phys Chem Chem Phys 12:5771–5778

    Article  CAS  Google Scholar 

  86. Zhou X, Graziani D, Pines A (2009) Proc Natl Acad Sci USA 106:16903–16906

    Article  CAS  Google Scholar 

  87. Cherubini A, Bifone A (2003) Prog Nucl Magn Reson Spectrosc 42:1–30

    Article  CAS  Google Scholar 

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Acknowledgements

This research work was supported by grants from Hong Kong Research Grants Council (RGC664109, RGC775508, and RGC776509), AoE/M-06/08, TUYF10SC03, SEG/CUHK09, SEG_HKUST06, and HKUST Research Fund.

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Authors and Affiliations

  1. Department of Microbiology, The University of Hong Kong, Pokfulam Road, Hong Kong, China

    Kong Hung Sze & Ho Sum Tse

  2. Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

    Qinglin Wu & Guang Zhu

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  1. Kong Hung Sze
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  2. Qinglin Wu
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  3. Ho Sum Tse
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  4. Guang Zhu
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Correspondence to Kong Hung Sze or Guang Zhu .

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Editors and Affiliations

  1. , Dept. of Biochemistry, Hong Kong University of Science and Tech, Clear Water Bay, Hong Kong, Kowloon, China, People's Republic

    Guang Zhu

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Sze, K.H., Wu, Q., Tse, H.S., Zhu, G. (2011). Dynamic Nuclear Polarization: New Methodology and Applications. In: Zhu, G. (eds) NMR of Proteins and Small Biomolecules. Topics in Current Chemistry, vol 326. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_297

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