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A Nanomembrane-Based Nucleic Acid Sensing Platform for Portable Diagnostics

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Microfluidics

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 304))

Abstract

In this perspective article, we introduce a potentially transformative DNA/RNA detection technology that promises to replace DNA microarray and real-time PCR for field applications. It represents a new microfluidic technology that fully exploits the small spatial dimensions of a biochip and some new phenomena unique to the micro- and nanoscales. More specifically, it satisfies all the requisites for portable on-field applications: fast, small, sensitive, selective, robust, label- and reagent-free, economical to produce, and possibly PCR-free. We discuss the mechanisms behind the technology and introduce some preliminary designs, test results, and prototypes.

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Abbreviations

AC:

Alternating current

CNT:

Carbon nanotube

DC:

Direct current

DEP:

Dielectrophoresis

DNA:

Deoxyribonucleic acid

FCW:

Fluorescence correlation spectroscopy

FET:

Field-effect transistor

I–V :

Current–Voltage

kb:

Kilobase

PCR:

Polymerase chain reaction

pM:

Picomolar

RNA:

Ribonucleic acid

SNP:

Single-nucleotide polymorphism

ssDNA:

Single-stranded DNA

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Acknowledgment

The authors are grateful to NSF, Great Lakes Protection Agency, Gates Foundation, NIH and ND-PDT for their generous support of this research.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of Notre Dame, 46556, Notre Dame, IN, USA

    Satyajyoti Senapati, Sagnik Basuray, Zdenek Slouka, Li-Jing Cheng & Hsueh-Chia Chang

Authors
  1. Satyajyoti Senapati
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  2. Sagnik Basuray
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  3. Zdenek Slouka
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  4. Li-Jing Cheng
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  5. Hsueh-Chia Chang
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Corresponding author

Correspondence to Hsueh-Chia Chang .

Editor information

Editors and Affiliations

  1. The Chinese Academy of Sciences, Laboratory of Biotechnology, Dalian Institute of Chemical Physics, ZhongShan Road 457, Dalian, 116023, China, People's Republic

    Bingcheng Lin

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Senapati, S., Basuray, S., Slouka, Z., Cheng, LJ., Chang, HC. (2011). A Nanomembrane-Based Nucleic Acid Sensing Platform for Portable Diagnostics. In: Lin, B. (eds) Microfluidics. Topics in Current Chemistry, vol 304. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_142

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