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Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells

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High Resolution Microbial Single Cell Analytics

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 124))

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Abbreviations

GSD microscopy:

Ground state depletion microscopy

GSDIM:

Ground state depletion microscopy followed by individual molecule return

MMP:

Mitochondrial membrane potential

PALM:

Photoactivated localization microscopy

RESOLFT:

Reversible saturable/switchable optical linear (fluorescence) transitions

STED microscopy:

Stimulated emission depletion microscopy

STORM:

Stochastic optical reconstruction microscopy

TOM:

Translocase of the outer membrane

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Acknowledgments

We thank C.A. Wurm for insightful discussions and providing some of the STED images. We also thank R. Schmidt and A. Egner regarding STED microscopy of mitochondria, S.W. Hell for continuous support and J. Jethwa for carefully reading the manuscript. Part of the work reported in this review was supported by the Bundesministerium für Bildung und Forschung (BMBF) (SysCompart, to S.J.).

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  1. Mitochondrial Structure and Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Goettingen, Germany

    Stefan Jakobs, Stefan Stoldt & Daniel Neumann

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Correspondence to Stefan Jakobs .

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  1. GmbH (UFZ), Dept. Umweltmikrobiologie, Helmholtz-Zentrum für Umweltforschung, Permoserstr. 15, Leipzig, 04318, Germany

    Susann Müller

  2. Fak. Maschinenwesen, Inst. Lebensmittel- und, TU Dresden, Bergstr. 120, Dresden, 01069, Sachsen, Germany

    Thomas Bley

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Jakobs, S., Stoldt, S., Neumann, D. (2010). Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells. In: Müller, S., Bley, T. (eds) High Resolution Microbial Single Cell Analytics. Advances in Biochemical Engineering / Biotechnology, vol 124. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2010_81

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